Antimonite [Sb(III)]-oxidizing bacteria can transform the toxic Sb(III) into less antimonate [Sb(V)]. Recently, cytoplasmic Sb(III)-oxidase AnoA and periplasmic arsenite [As(III)] oxidase AioAB were shown to responsible for bacterial oxidation, however, disruption of each gene only partially decreased oxidation efficiency. This study showed that in Agrobacterium tumefaciens GW4, induced cellular H2O2 content degradation katA. Gene knock-out/complementation katA, anoA, aioA anoA/aioA growth...

Agrobacterium tumefaciens GW4 is a heterotrophic arsenite [As(III)]/antimonite [Sb(III)]-oxidizing strain. The As(III) oxidase AioAB responsible for oxidation in the periplasm and it also involved Sb(III) 5A. In addition, AnoA cellular H2O2 are strain GW4. However, deletion of aioA increased efficiency present study, we found that cell mobility to Sb(III), ATP NADH contents heat release were by more significantly mutant. Proteomics transcriptional analyses showed proteins/genes resistance,...

The middle and lower reaches of the Yangtze River basin (MLRYB) are prone to flooding because their orientation is parallel East Asian summer monsoon rain belt. Since presents pronounced intraseasonal variability, subseasonal prediction precipitation anomalies in MLRYB region an imperative demand nationwide. Based on rotated empirical orthogonal function analysis, 48 stations over with coherent (10–80-day) rainfall variability identified. Power spectrum analysis index, defined as...

A Gram-staining-negative, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated WG4T, was isolated from soil of the Tianmen Mountain located in Hunan province, PR China. 16S rRNA gene sequence analysis showed that strain belongs to genus Chryseobacterium family Flavobacteriaceae, with 97.4 % and 97.1 identities anthropi NF 1366T haifense H38T, respectively. In comparison other strains representing Chryseobacterium, were less than 97.0 %. The DNA-DNA relatedness values 63.3...

A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521T was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that belonged to genus Nocardioides in family Nocardioidaceae, with the highest sequence identity panacisoli GSoil 346T (96.3 %). The DNA G+C content of 70.3 mol%. cell-wall peptidoglycan type ll-2,6-diaminopimelic acid. major respiratory quinone MK-8(H4), and predominant fatty acids (>5 %) were iso-C16 : 0, C18 1ω9c C17 1ω8c....

SummaryThe glutathione S-transferases (GSTs) have been recognized to play an important role in the detoxification of xenobiotics. The tissue distribution and activities GSTs Apis cerana Fabricius mellifera ligustica Spinola were assayed using 1-chloro-2,4- dinitrobenzene (CDNB) as substrate. present at all developmental stages honeybee widely distributed different organs. from midgut both species showed highest activity among detected enzymes preferentially cytosol fraction midgut. each...

A Gram-stain-negative, rod-shaped, motile by gliding and strictly aerobic bacterial strain, named 1-32T, was isolated from soil of the Ordos grassland in Inner Mongolia, PR China. Strain 1-32T showed highest 16S rRNA gene sequence similarities to Pedobacter luteus N7d-4T (95.4 %), oryzae DSM 19973T (95.3 'Pedobacter xinjiangensis' 12157T (95.2 %) tournemirensis TF5-37.2-LB10T (95.1 %). Phylogenetic analyses clustered strain with 'P. P. TF5-37.2-LB10T. The DNA G+C content 43.4 mol%....

Purpose: To investigate the effect of Astragalus membranaceus (Fisch.) Bunge. extract (AMBE) on streptozotocin-induced diabetic rats.Methods: The aqueous AMB was obtained by steeping dried in water at 60 oC three times, each for 1 h, before first drying an oven 100 and then freeze-drying last thus obtained. Diabete model rats induced a single intraperitoneal injection freshly prepared solution streptozotocin (50 mg/kg). were randomly divided into 6 groups ten each: negative control group,...

Both the calcium and collagen in bone powder are hard to be absorbed by body. Although enzymatic hydrolysis protease increased bio-availability of powder, it was a meaningful try further increase Ca2+ release, oligopeptide formation antioxidant activity sheep hydrolysate (SBH) lactic acid bacteria (LAB) fermentation. Lactobacillus helveticus selected as starter for its highest protease-producing ability among 5 tested LAB strains. The content liberated measured responsive value response...

Paradiplozoon cirrhini n. sp. (Monogenea, Diplozoidae) is described from the gills of mud carp, Cirrhinus molitorella (Valenciennes, 1844) (Cyprinidae, Labeoninae), collected in Wuzhou, Guangxi Province, and Conghua, Guangdong Province as part an ongoing survey diplozoid fauna Pearl River basin China. The new species distinguished congeners by structure median plate its outgrowth sclerites. ITS2 sequences differ all known available 22.04%–38.34%. first parasitic on Labeoninae Molecular...

Intervertebral disc degeneration (IVDD) is a multifaceted condition characterized by heterogeneity, wherein the balance between catabolism and anabolism in extracellular matrix of nucleus pulposus (NP) cells plays central role. Presently, available treatments primarily focus on relieving symptoms associated with IVDD without offering an effective cure targeting its underlying pathophysiological processes. D-mannose (referred to as mannose) has demonstrated anti-catabolic properties various...

Paracaesicola n. gen., is erected herein to accommodate a new microcotylid species, nanshaensis sp., collected from the Yongshu Reef, South China Sea. This species first monogenean be recorded gills of Paracaesio sordida . The characterized by following features: (i) haptor short, with clamps arranged in two equal bilateral rows; (ii) testes numerous, roughly alternating longitudinal rows, extending into haptor; (iii) genital atrium armed 16 robust spines, which are vertically on top sausage...

Parasite biodiversity of fish in coral reefs the South China Sea is still incompletely explored. We describe here a new species Neohexostoma (Monogenea: Hexostomatidae) from gill filaments dogtooth tuna Gymnosarda unicolor (Scombridae), collected off Yongshu Reef, Sea. gymnosardae n. sp. distinguished its congeners by following features: (i) haptor clearly marked body proper strongly constricted peduncle, divided posterior margin into two symmetrical lobes, (ii) vagina armed with scattered...

Yeast supplementation has been shown to increase feed intake and production in some studies with early lactation dairy cows, but the mechanisms underlying this effect remain unknown. The objective of study was assess effects supplementing a yeast product derived from Saccharomyces cerevisiae on production, feeding behavior, immune function cows during transition lactation. When fed for 3 weeks before calving through 6 after calving, altered behavior as well responsiveness vaccination gut...

Feeding chromium in early lactation can increase milk production, but responses during peak have not been studied. The objective of this study was to evaluate propionate period as well interactions with rumen-protected lysine and methionine. Chromium increased feed intake tended energy-corrected yield, primiparous cows showed greater protein yield than multiparous cows. In study, feeding near productivity, no benefits supplementing methionine were observed.; Dairy Day, 2013, Kansas State...