A5044859624- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Chemical Synthesis and Analysis
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- Biotin and Related Studies
- Mycobacterium research and diagnosis
- Plant Disease Resistance and Genetics
- Ubiquitin and proteasome pathways
- Antimicrobial Peptides and Activities
- Endoplasmic Reticulum Stress and Disease
- Coagulation and Flocculation Studies
- Glycosylation and Glycoproteins Research
- Plant Pathogenic Bacteria Studies
- Diabetes Treatment and Management
- Receptor Mechanisms and Signaling
- Genetic Neurodegenerative Diseases
- Vehicle Routing Optimization Methods
- Cardiovascular Function and Risk Factors
- Legume Nitrogen Fixing Symbiosis
- Fungal and yeast genetics research
- Sirtuins and Resveratrol in Medicine
Novo Nordisk (United Kingdom)
2023
Novo Nordisk (Denmark)
2022-2023
Agro Business Park
2020
University of Copenhagen
2009-2015
Novo Nordisk Foundation
2012
Aarhus University
2007-2009
University of Southern Denmark
2003-2005
Obesity-related heart failure with preserved ejection fraction (HFpEF) has become a well-recognized HFpEF subphenotype. Targeting the unfavorable cardiometabolic profile may represent rational treatment strategy. This study investigated semaglutide, glucagon-like peptide-1 receptor agonist that induces significant weight loss in patients obesity and/or type 2 diabetes mellitus and been associated improved cardiovascular outcomes. In mouse model of was caused by advanced aging, female sex,...
Virus-induced gene silencing (VIGS) can be used as a powerful tool for functional genomics studies in plants. With this approach, it is possible to target most genes and downregulate the messenger (m)RNA sequence-specific manner. Barley stripe mosaic virus (BSMV) an established VIGS vector barley wheat; however, using generally transient, with efficient often being confined first two or three systemically infected leaves. To investigate further, part of Phytoene desaturase (PDS) was inserted...
Abstract Peptides play important roles in regulating biological processes and form the basis of a multiplicity therapeutic drugs. To date, only about 300 peptides human have confirmed bioactivity, although tens thousands been reported literature. The majority these are inactive degradation products endogenous proteins peptides, presenting needle-in-a-haystack problem identifying most promising candidate from large-scale peptidomics experiments to test for bioactivity. address this challenge,...
Abstract The essential vitamin biotin is a covalent and tenaciously attached prosthetic group in several carboxylases that play important roles the regulation of energy metabolism. Here we describe increased acetyl-CoA levels mitochondrial hyperacetylation as downstream metabolic effects deficiency. Upregulated acetylation sites correlate with cellular deficiency Hst4p deacetylase, biotin-starvation-induced accumulation mitochondria supports role for lowering acetylation. We show starvation...
There are three sites of m(5)U modification in Escherichia coli stable RNAs: one at the invariant tRNA position U54 and two 23S rRNA phylogenetically conserved positions U747 U1939. Each these is modified by its own methyltransferase, TrmA, well-characterised. Two open reading frames, YbjF YgcA, approximately 30% identical to here we determine functions candidate methyltransferases using MALDI mass spectrometry. A purified recombinant version YgcA retains activity specificity, methylates...
Members of the Mycobacterium tuberculosis complex possess a resistance determinant, erm(37) (also termed ermMT), which is truncated homologue erm genes found in diverse range drug-producing and pathogenic bacteria. All examined thus far encode N(6)-monomethyltransferases or N(6),N(6)-dimethyltransferases that show absolute specificity for nucleotide A2058 23 S rRNA. Monomethylation at confers to subset macrolide, lincosamide, streptogramin B (MLS(B)) group antibiotics no latest macrolide...
Protein digestion is an integral part of the "shotgun" proteomics approach and commonly requires overnight incubation prior to mass spectrometry analysis. Quadruplicate proteomic analysis whole yeast lysate demonstrated that Guanidine-Hydrochloride (Gnd-HCl) protein can be optimally completed within 30 min with endoprotease Lys-C. No chemical artifacts were introduced when samples incubated in Gnd-HCl at 95 °C, making appropriate buffer for shotgun proteomics. Current methodologies...
A mutation, L166P, in the cytosolic protein, PARK7/DJ-1, causes protein misfolding and is linked to Parkinson disease. Here, we identify fission yeast Sdj1 as orthologue of DJ-1 calculate by silico saturation mutagenesis effects point mutants on its structural stability. We also map degradation pathways for Sdj1-L169P, disease-causing L166P protein. Sdj1-L169P forms inclusions, which are enriched Hsp104 disaggregase. Hsp70-type chaperones required efficient Sdj1-L169P. This depends...
The waxy cell walls of mycobacteria provide intrinsic tolerance to a broad range antibiotics, and this effect is augmented by specific resistance determinants. inducible determinant erm(38) in the nontuberculous species Mycobacterium smegmatis confers high lincosamides some macrolides, without increasing streptogramin B antibiotics. This an uncharacteristic pattern falling between type I II macrolide, lincosamide, (MLS(B)) phenotypes that are conferred, respectively, Erm...
The problem of finding an optimal solution for the slurry application process is casted as a capacitated vehicle routing (CVRP) in which by considering vehicle’s capacity, it required to visit all tracks only once fully cover field, well complying with specified targeted rate. A key objective this study was determine optimized coverage plan order minimize driving distance while at same time allowing varying includes sequence rate each track. Two algorithms were developed optimization and...
Pectin methylesterases (PMEs) catalyse the removal of methyl esters from homogalacturonan (HG) backbone domain pectin, a ubiquitous polysaccharide in plant cell walls. The degree esterification (DE) impacts upon functional properties HG within walls and plants produce numerous PMEs that act muro. Many microbial pathogens also PMEs, activity which renders more susceptible to cleavage by pectin lyase polygalacturonase enzymes hence aids wall degradation. We have developed novel...
Abstract Motivation Peptides are ubiquitous throughout life and involved in a wide range of biological processes, ranging from neural signaling higher organisms to antimicrobial peptides bacteria. Many generated post-translationally by cleavage precursor proteins can thus not be detected directly genomics data, as the specificities responsible proteases often completely understood. Results We present DeepPeptide, deep learning model that predicts cleaved amino acid sequence. DeepPeptide...
Abstract Many secreted endogenous peptides rely on signalling pathways to exert their function in the body. While can be discovered through high throughput technologies, cognate receptors typically cannot, hindering understanding of mode action. We investigate use AlphaFold-Multimer for identifying human receptor libraries without any prior knowledge about likely candidates. find that AlphaFold’s predicted confidence metrics have strong performance prioritizing true peptide-receptor...