A5008801222- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Pharmacogenetics and Drug Metabolism
- Single-cell and spatial transcriptomics
- Drug-Induced Hepatotoxicity and Protection
- Drug Transport and Resistance Mechanisms
- MicroRNA in disease regulation
- Immune cells in cancer
- Effects and risks of endocrine disrupting chemicals
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Cancer Genomics and Diagnostics
- Pharmacological Effects and Toxicity Studies
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- RNA Research and Splicing
- Extracellular vesicles in disease
- Animal testing and alternatives
- Carcinogens and Genotoxicity Assessment
- Peroxisome Proliferator-Activated Receptors
- Pharmaceutical and Antibiotic Environmental Impacts
- SARS-CoV-2 detection and testing
- Hepatitis C virus research
- RNA regulation and disease
- Toxic Organic Pollutants Impact
Rapt Therapeutics (United States)
2024
University of California, San Francisco
2021-2023
Université de Rennes
2013-2022
Centre Hospitalier Universitaire de Rennes
2018-2022
Institut de Recherche en Santé, Environnement et Travail
2013-2022
UCSF Helen Diller Family Comprehensive Cancer Center
2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020
Nutrition, métabolismes et cancer
2020
Inserm
2013-2020
Sanofi (France)
2018
Pediatric hepatoblastoma is the most common primary liver cancer in infants and children. Studies of that focus exclusively on tumor cells demonstrate sparse somatic mutations a cell origin, hepatoblast, across patients. In contrast to homogeneity these studies would suggest, tumors have high degree heterogeneity can portend poor prognosis. this study, we use single-cell transcriptomic techniques analyze resected human pediatric specimens, identify five signatures may account for observed...
Abstract Hepatic steatosis (i.e. lipid accumulation) and steatohepatitis have been related to diverse etiologic factors, including alcohol, obesity, environmental pollutants. However, no study has so far analyzed how these different factors might interplay regarding the progression of liver diseases. The impact co-exposure carcinogen benzo[a]pyrene (B[a]P) lifestyle-related hepatotoxicant ethanol, was thus tested on in vitro models (human HepaRG cell line; hybrid human/rat WIF-B9 line), an...
Exposure to xenobiotics could favor the transition of nonalcoholic fatty liver (NAFL) steatohepatitis in obese patients. Recently, we showed different models NAFL that benzo[a]pyrene (B[a]P) and ethanol coexposure induced a steatohepatitis-like state. One model was HepaRG cells incubated with stearate oleate for 2 weeks. In present study, wished determine this whether mitochondrial dysfunction reactive oxygen species (ROS) overproduction be involved occurrence CRISPR/Cas9-modified were also...
Abstract Steatosis is a liver lesion reported with numerous pharmaceuticals. Prior studies showed that severe impairment of mitochondrial fatty acid oxidation (mtFAO) constantly leads to lipid accretion in liver. However, much less known about the mechanism(s) drug-induced steatosis absence dysfunction, although previous suggested involvement mild-to-moderate inhibition mtFAO, increased de novo lipogenesis (DNL), and very low-density lipoprotein (VLDL) secretion. The objective our study,...
Although mitochondriotoxicity plays a major role in drug-induced hepatotoxicity, alteration of mitochondrial DNA (mtDNA) homeostasis has been described only with few drugs. Because it requires long drug exposure, this mechanism toxicity cannot be detected investigations performed isolated liver mitochondria or cultured cells exposed to drugs for several hours days. Thus, first aim study was determine whether 2-week treatment nine hepatotoxic could affect mtDNA HepaRG cells. Previous these...
Abstract Extracellular vesicles (EVs) are membrane-enclosed nanostructures released by cells into the extracellular environment. As major actors of physiological intercellular communication, they have been shown to be pathogenic mediators several liver diseases. also appear potential drug-induced injury but nothing is known concerning environmental pollutants. We aimed study impact polycyclic aromatic hydrocarbons (PAHs), contaminants, on hepatocyte-derived EV production, with a special...
Human exposure to bisphenol A (BPA) could favor obesity and related metabolic disorders such as hepatic steatosis. Investigations in rodents have shown that these deleterious effects are observed not only when BPA is administered during the adult life but also with different protocols of perinatal exposure. Whether pose a risk human currently unknown, thus appropriate vitro models be important tackle this major issue. Accordingly, we determined whether long-term treatment induce steatosis...
Organochlorine (OC) pesticides constitute a major class of persistent and toxic organic pollutants, known to modulate drug-detoxifying enzymes. In the present study, OCs were demonstrated also alter activity expression human hepatic drug transporters. Activity sinusoidal influx transporter OCT1 (organic cation 1) was thus inhibited by endosulfan, chlordane, heptachlor, lindane, dieldrine, but not dichlorodiphenyltrichloroethane isomers, whereas those canalicular efflux pumps MRP2 (multidrug...
Abstract Background The antineoplastic drug busulfan can induce different hepatic lesions including cholestasis and sinusoidal obstruction syndrome. However, steatosis has never been reported in patients. Objectives This study aimed to determine whether could primary human hepatocytes (PHH) differentiated HepaRG cells. Methods Neutral lipids were determined PHH Mechanistic investigations performed cells by measuring metabolic fluxes linked lipid homeostasis, reduced glutathione (GSH) levels,...
Infection by coronavirus SARS-CoV2 is a severe and often deadly disease that has implications for the respiratory system multiple organs across human body. While effects in lung have been extensively studied, less known about impact COVID-19 other organs. Here, we contribute single-nuclei RNA-sequencing atlas comprising six 20 autopsies where analyzed transcriptional changes due to cell types. The integration of data from enabled identification systemic changes. Computational cross-organ...
Background: Apolipoprotein C-III (APOC3) is a key regulator of lipid metabolism that inhibits the catabolism and clearance triglyceride (TG)-rich lipoproteins in blood. Loss-of-function APOC3 variants are associated with significantly lower TG levels reduced risk heart disease, underscoring potential inactivation as therapeutic strategy for patients familial chylomicronemia syndrome (FCS) severe hypertriglyceridemia (SHTG). Here, we developed STX-1400 lead molecules novel investigational...
Abstract Infection by Coronavirus SARS-CoV2 is a severe and often deadly disease that has implications for the respiratory system multiple organs across human body. While effects in lung have been extensively studied, less known about COVID-19’s cellular impact other organs. Here we contribute single-nuclei RNA sequencing atlas comprising six 20 autopsies where analyzed transcriptional changes due to COVID-19 cell types. Computational cross-organ analysis endothelial cells macrophages...
ABSTRACT Pediatric hepatoblastoma (HB) is the most common primary liver cancer in infants and children. Studies of HB that focus exclusively on tumor cells demonstrate sparse somatic mutations a cell origin, hepatoblast, across patients. In contrast to homogeneity these studies would suggest, tumors have high degree heterogeneity can portend poor prognosis. this study, we used single-cell genomic techniques analyze resected human pediatric specimens. This study establishes be defined by...