A5101765381- Immune Cell Function and Interaction
- interferon and immune responses
- T-cell and Retrovirus Studies
- NF-κB Signaling Pathways
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Cancer Cells and Metastasis
- Cancer-related molecular mechanisms research
- Respiratory viral infections research
- RNA Research and Splicing
- Lymphoma Diagnosis and Treatment
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Seismic Imaging and Inversion Techniques
- Synthesis of heterocyclic compounds
- Wnt/β-catenin signaling in development and cancer
- Histone Deacetylase Inhibitors Research
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Chromatin Remodeling and Cancer
- Sphingolipid Metabolism and Signaling
The University of Texas Medical Branch at Galveston
2011-2024
Third Affiliated Hospital of Nanchang University
2021-2024
Wenzhou Medical University
2023-2024
Zhejiang Taizhou Hospital
2024
Third Hospital of Nanchang
2021-2024
Nanchang University
2021-2024
China National Petroleum Corporation (China)
2022
University of Virginia Cancer Center
2018
University of Virginia
2017-2018
Guangzhou Medical University
2016
Colon cancer is the second leading cause of death in United States. Krüppel-like factor 4 (KLF4) a transcription involved both proliferation and differentiation colon. It down-regulated mouse human colonic adenomas has been implicated as tumor suppressor gut, whereas breast cancer, KLF4 an oncogene. also reprogramming differentiated cells into pluripotent stem cells. can act transcriptional activator or repressor, but underlying mechanisms are poorly understood. We found that p300,...
Most colorectal cancers have mutations of the adenomatous polyposis coli (APC) gene or beta-catenin that stabilize and activate target genes, leading ultimately to cancer. The molecular mechanisms APC function in degradation are not completely known. binds is involved Axin complex, suggesting regulates phosphorylation. Some evidence also suggests nuclear export. Here, we examine effects on phosphorylation, ubiquitination, colon cancer cell lines SW480, DLD-1, HT29, each which contains a...
Chronic epithelial injury triggers a TGF-β-mediated cellular transition from normal epithelium into mesenchymal-like state that produces subepithelial fibrosis and airway remodeling. Here we examined how TGF-β induces the mesenchymal cell determined its mechanism. We observed stimulation activates an inflammatory gene program controlled by NF-κB/RelA signaling pathway. In state, NF-κB-dependent immediate-early genes accumulate euchromatin marks processive RNA polymerase. This of is activated...
A central question in Wnt signaling is the regulation of beta-catenin phosphorylation and degradation. Multiple kinases, including CKI alpha GSK3, are involved phosphorylation. Protein phosphatases such as PP2A PP1 have been implicated beta-catenin. However, which phosphatase dephosphorylates vivo how specificity dephosphorylation regulated not clear. In this study, we show that regulates degradation vivo. We demonstrate required for Wnt/beta-catenin Drosophila. Moreover, identified PR55...
ABSTRACT The airway mucosa expresses protective interferon (IFN) and inflammatory cytokines in response to respiratory syncytial virus (RSV) infection. In this study, we examine the role of bromodomain containing 4 (BRD4) mediating innate immune human small epithelial cells. We observe that RSV induces BRD4 complex with NF-κB/RelA. is functionally required for expression NF-κB-dependent gene regulatory network (GRN), including IFN factor 1 (IRF1) IRF7, which mediate a cross talk pathway...
The airway epithelial cell plays a central role in coordinating the pulmonary response to injury and inflammation. Here, transforming growth factor-β (TGFβ) activates gene expression programs induce stem cell-like properties, inhibit of differentiated adhesion proteins express mesenchymal contractile proteins. This process is known as transition (EMT); although much about EMT cellular metastasis an oncogene-transformed cell, less Type II EMT, that occurring normal cells. In this study, we...
Abstract Histone deacetylases (HDACs) are involved in tumor progression, and some have been successfully targeted for cancer therapy. The expression of histone deacetylase 4 (HDAC4), a class IIa HDAC, was upregulated our previous microarray screen. However, the role HDAC4 dysregulation mechanisms underlying growth metastasis nasopharyngeal carcinoma (NPC) remain elusive. Here, we first confirmed that levels primary metastatic NPC tissues were significantly increased compared with those...
Large granular lymphocyte (LGL) leukemia is a clonal proliferative disease of T and natural killer (NK) cells. Interleukin (IL)-15 important for the development progression LGL survival factor normal NK memory IL-15 alters expression Bcl-2 family members, Bcl-2, Bcl-XL, Bim, Noxa, Mcl-1; however, effects on Bid have not been shown. Using an adoptive transfer model, we show that cells from Bid-deficient mice survive longer than wild-type control when transferred into IL-15-null mice. In human...
Lower respiratory tract infection with syncytial virus (RSV) produces profound inflammation. Despite an understanding of the role adaptive immunity in RSV infection, identity major sentinel cells initially triggering inflammation is controversial. Here we evaluate nonciliated secretoglobin (Scgb1a1)-expressing bronchiolar epithelial infection. Mice expressing a tamoxifen (TMX)-inducible Cre recombinase-estrogen receptor fusion protein (CreERTM) knocked into Scgb1a1 locus were crossed mice...
The mechanisms by which the mammalian airway detects invading viral pathogens to trigger protective innate neutrophilic inflammation are incompletely understood. We observe that activation of nuclear factor κB (NF-κB)/RelA transcription indirectly activates atypical BRD4 histone acetyltransferase (HAT) activity, RNA polymerase II (Pol II) phosphorylation, and secretion chemokines. To study this pathway in vivo, we developed a conditional knockout RelA distal epithelial cells; these animals...
Inducible transcriptional elongation is a rapid, stereotypic mechanism for activating immediate early immune defense genes by the epithelium in response to viral pathogens. Here, recruitment of multifunctional complex containing cyclin dependent kinase 9 (CDK9) triggers process resting RNA polymerase engaged with innate (IIR) genes. To identify additional functional activity CDK9 complex, we conducted immunoprecipitation (IP) enrichment-stable isotope labeling LC-MS/MS unstimulated cells and...
Abstract Context: Endothelial microRNA 126 (miR-126) attenuates the development of atherosclerosis (AS). However, there is no evidence showing role miR-126 in estrogen’s antiatherogenic effects. Objective: We hypothesized that 17β-estradiol (E2) modulates expression and thus may improve endothelial function retard AS development. Design/Setting/Participants: This was a prospective cohort study 12 healthy regularly menstruating female volunteers. ApoE−/− mice were used as model human...
Using nuclear factor-κB (NF-κB) ChIP-Seq data, we present a framework for iterative learning of regulatory networks. For every possible transcription factor-binding site (TFBS)-putatively regulated gene pair, the relative distance and orientation are calculated to learn which TFBSs most likely regulate given gene. Weighted TFBS contributions putative regulation integrated derive an NF-κB network. A d e novo motif enrichment analysis uncovers secondary (AP1, SP1) at characteristic distances...
Human polo-like kinase 1 (PLK1) has been recognized as an attractive therapeutic target against nasopharyngeal carcinoma (NPC). The contains a conserved polo-box domain (PBD) that exhibits wide specificity across various substrates. Previously, we explored natural amino acid preference in PLK1 PBD-binding phosphopeptides. However, limited to the short sequence only acids cannot guarantee sufficient exploitation of chemical and structural diversity Here, described genetic optimization (GO)...