A5031450029- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Characterization and Applications of Magnetic Nanoparticles
- Protein Degradation and Inhibitors
- PARP inhibition in cancer therapy
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Chromatin Remodeling and Cancer
- Geomagnetism and Paleomagnetism Studies
- Magnetic properties of thin films
- Sirtuins and Resveratrol in Medicine
- Bacteriophages and microbial interactions
Boston VA Research Institute
2023-2025
Harvard University
2023-2025
University of Colorado Boulder
2020-2022
Howard Hughes Medical Institute
2020
University of Würzburg
2019
The reversible acetylation of histone lysine residues is controlled by the action acetyltransferases and deacetylases (HDACs), which regulate chromatin structure gene expression. sirtuins are a family NAD-dependent HDAC enzymes, one member, sirtuin 6 (Sirt6), influences DNA repair, transcription, aging. Here, we demonstrate that Sirt6 efficient at deacetylating several H3 sites, including its canonical site Lys9, in context nucleosomes but not free acetylated protein substrates. By...
Abstract Magnetic Particle Imaging (MPI) is a promising new tomographic modality for fast as well three-dimensional visualization of magnetic material. For anatomical or structural information an additional imaging such computed tomography (CT) required. In this paper, the first hybrid MPI-CT scanner multimodal providing simultaneous data acquisition presented.
Epigenetic inheritance requires the transfer of parental histones to newly synthesized DNA during eukaryotic chromosome replication, yet structural mechanisms underlying replisome engagement with nucleosomes remain unclear. Here we establish an in vitro chromatin replication system and report four cryo-EM structures human complex a nucleosome. The capture distinct states nucleosomal unwrapping nucleosome integrity disassembly by encroaching replisome.
An ATP-dependent remodeler engages nucleosomes in a unique manner.
Abstract Acetylation of histones is a key post-translational modification that guides gene expression regulation. In yeast, the class I histone deacetylase containing Rpd3S complex plays critical role in suppression spurious transcription by removing acetylation from actively transcribed genes. The S. cerevisiae has five subunits (Rpd3, Sin3, Rco1, Eaf3, and Ume1) but its subunit stoichiometry how engages nucleosomes to achieve substrate specificity remains elusive. Here we report cryo-EM...
Acetylation of histones is a key post-translational modification that guides gene expression regulation. In yeast, the class I histone deacetylase containing Rpd3S complex plays critical role in suppression spurious transcription by removing acetylation from actively transcribed genes. The Saccharomyces cerevisiae has five subunits (Rpd3, Sin3, Rco1, Eaf3, and Ume1) but its subunit stoichiometry how engages nucleosomes to achieve substrate specificity remains elusive. Here we report cryo-EM...
During transcription, RNA polymerase II traverses through chromatin, and post-translational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine 36 trimethylation (H3K36me3), which is established by the methyltransferase SETD2, suppresses cryptic regulates splicing, serves as a binding site for transcription elongation factors. The mechanism machinery coordinates deposition H3K36me3 not well understood. Here we provide cryo-electron...
Abstract The ATP-dependent chromatin remodeler SMARCAD1 acts on nucleosomes during DNA repair and transcription, but despite its implication in disease, information structure function is scarce. Chromatin remodelers use a variety of ways to engage nucleosomes, outcomes the reactions vary widely. Here we show that transfers entire histone octamer from one segment another an manner also capable de novo nucleosome assembly octamer, due ability bind all histones simultaneously. We describe...
All eukaryotes organize their DNA into nucleosomes, consisting of an octamer the four core histone proteins H2A, H2B, H3, and H4, around which 147 base pairs are wrapped in two tight superhelical turns. Nucleosomes pack higher order structures require chaperones ATP-dependent remodelers to make accessible. I will discuss our recent progress understanding structure chromatin at centromere, role centromere-binding locally folding fiber. also mechanisms by nucleosomes remodeled assembled ATP...