A5020086828- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Plant Molecular Biology Research
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Developmental Biology and Gene Regulation
- Vascular anomalies and interventions
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Congenital Diaphragmatic Hernia Studies
- Congenital Heart Disease Studies
- Insect and Arachnid Ecology and Behavior
- Pluripotent Stem Cells Research
- Pancreatic and Hepatic Oncology Research
- Chromosomal and Genetic Variations
- Renal and Vascular Pathologies
- Protein Degradation and Inhibitors
- Gastrointestinal disorders and treatments
- Intestinal Malrotation and Obstruction Disorders
- Protein Structure and Dynamics
Harvard University
2022-2024
Boston VA Research Institute
2022-2024
University of Missouri–Kansas City
2024
Stowers Institute for Medical Research
2016-2021
Maastricht University
2014-2015
Abstract Background It remains unclear to what extent midgut rotation determines human intestinal topography and pathology. We reinvestigated the during its looping herniation phases of development, using novel 3D visualization techniques. Results distinguished 3 generations loops. The primary secondary loops was constant, but that tertiary not. orientation loop changed from sagittal transverse due descent ventral structures in a body with still helical axis. 1 st (duodenum, proximal...
In eukaryotes, RNA polymerase (Pol) II transcribes chromatin and must move past nucleosomes, often resulting in nucleosome displacement. How Pol unwraps the DNA from nucleosomes to allow transcription how rewraps retain has been unclear. Here, we report 3.0-angstrom cryo-electron microscopy structure of a mammalian II-DSIF-SPT6-PAF1c-TFIIS-nucleosome complex stalled 54 base pairs within nucleosome. The provides mechanistic basis for retention during elongation where upstream emerging cleft...
Understanding the developmental changes in neuronal lineages is crucial to elucidate how they assemble into functional neural networks. Studies investigating nervous system development model systems have focused on only a few regions of central due limited availability genetic drivers that target specific throughout and adult life. This has hindered our understanding distinct interconnect form circuits during development. Here, we present split-GAL4 library composed driver lines, which...
Understanding the developmental changes in neuronal lineages is crucial to elucidate how they assemble into functional neural networks. Studies investigating nervous system development model systems have focused on only a few regions of central due limited availability genetic drivers that target specific throughout and adult life. This has hindered our understanding distinct interconnect form circuits during development. Here, we present split-GAL4 library composed driver lines, which...
The Gcn5 acetyltransferase functions in multiple complexes yeast and metazoans. Yeast is part of the large SAGA (Spt-Ada-Gcn5 acetyltransferase) complex a smaller ADA complex. In flies mammals, (and its homolog pCAF) various versions another complex, ATAC (Ada2A containing complex). However, analogous to small has never been described Previous studies Drosophila hinted at existence which contains Ada2b, partner Here we have purified characterized composition this show that it composed Gcn5,...
Abstract Differences in opinion regarding the development of infrahepatic inferior caval and azygos venous systems mammals centre on contributions ‘caudal cardinal’, ‘subcardinal’, ‘supracardinal’, ‘medial lateral sympathetic line’ ‘sacrocardinal’ veins. The disagreements appear to arise from use topographical position rather than developmental origin as criterion define separate systems. We reinvestigated issue a closely spaced series human embryos between 4 10 weeks development. Structures...
Mouse embryonic stem cells (ESCs) cultured in defined medium resemble the pre-implantation epiblast ground state, with full developmental capacity including germline. β-Catenin is required to maintain state pluripotency mouse ESCs, but its exact role controversial. Here, we reveal a Tcf3-independent of β-catenin restraining germline and somatic lineage differentiation genes. We show that binds target genes E2F6 forms complex HMGA2 or HP1γ. Our data indicate these complexes help restrain...
Abstract Understanding the developmental trajectories of neuronal lineages is crucial for elucidating how they are assembled into functional neural networks. Studies investigating nervous system development in model animals have focused only on a few regions Central Nervous System due to limited availability genetic drivers target these throughout and adult life. This hindered our understanding distinct come together form circuits during development. Here, we present split-GAL4 library...
During transcription, RNA polymerase II traverses through chromatin, and post-translational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine 36 trimethylation (H3K36me3), which is established by the methyltransferase SETD2, suppresses cryptic regulates splicing, serves as a binding site for transcription elongation factors. The mechanism machinery coordinates deposition H3K36me3 not well understood. Here we provide cryo-electron...
Abstract The Spt/Ada-Gcn5 Acetyltransferase (SAGA) coactivator complex has multiple modules with different enzymatic and non-enzymatic functions. How each module contributes to gene activation in specific biological contexts is not well understood. Here we analyzed the role of core during Drosophila oogenesis. We show that depletion several SAGA-specific subunits belonging blocked egg chamber development mid-oogenesis stages, resulting stronger phenotypes than those obtained after SAGA’s...
The Spt/Ada-Gcn5 Acetyltransferase (SAGA) coactivator complex has multiple modules with different enzymatic and non-enzymatic functions. How each module contributes to gene expression is not well understood. During Drosophila oogenesis, the functions are equally required, which may indicate that genes require An analogy for this phenomenon handyman principle: while a many tools, tool he uses depends on what requires maintenance. Here we analyzed role of core during interacts TBP. We show...