A5009062511- Neuroendocrine regulation and behavior
- Autism Spectrum Disorder Research
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- Genetics and Neurodevelopmental Disorders
- Health Systems, Economic Evaluations, Quality of Life
- Animal testing and alternatives
- Meta-analysis and systematic reviews
- Biosimilars and Bioanalytical Methods
- Alzheimer's disease research and treatments
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Tryptophan and brain disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Clinical practice guidelines implementation
- Trauma and Emergency Care Studies
- Neural Networks and Applications
- Biotin and Related Studies
- DNA and Biological Computing
- Cardiac Arrest and Resuscitation
- Cell Image Analysis Techniques
- Health and Medical Research Impacts
- Evolutionary Algorithms and Applications
- Virology and Viral Diseases
- Electronic Health Records Systems
Broad Institute
2020-2021
Psychogenics (United States)
2015-2020
Cohen Veterans Bioscience
2019
New York State Psychiatric Institute
2011-2015
Columbia University
2011-2015
New York Psychoanalytic Society and Institute
2011
Autism spectrum disorder comprises several neurodevelopmental conditions presenting symptoms in social communication and restricted, repetitive behaviors. A major roadblock for drug development autism is the lack of robust behavioral signatures predictive clinical efficacy. To address this issue, we further characterized, a uniform rigorous way, mouse models that are interest because their construct validity wide availability to scientific community. We implemented broad battery included but...
While high risk of failure is an inherent part developing innovative therapies, it can be reduced by adherence to evidence-based rigorous research practices. Supported through the European Union’s Innovative Medicines Initiative, EQIPD consortium has developed a novel preclinical quality system that applied in both public and private sectors free for anyone use. The Quality System was designed suited boost innovation ensuring generation robust reliable data while being lean, effective not...
In adult animals, the medial prefrontal cortex (mPFC) plays a significant role in regulating emotions and projects to amygdala periaqueductal gray (PAG) modulate emotional responses. However, little is known about development of this neural circuit its relevance unlearned fear pre-adulthood. To address these issues, we examined mPFC 14-d-old (infants), 26-d-old (juveniles), 38- 42-d-old (adolescents) rats represent different developmental social milestones. The expression patterns neuronal...
To expand, analyze and extend published behavioral phenotypes relevant to autism spectrum disorder (ASD), we present a study of three ASD genetic mouse models: Feng's Shank3tm2Gfng model, hereafter Shank3/F, Jiang's Shank3tm1Yhj Shank3/J the Cacna1c deletion model. The Shank3 models mimick gene mutations associated with Phelan-McDermid Syndrome model recapitulates underlying Timothy syndrome. This utilizes both standard novel tests same methodology used in our previously companion report on...
Traumatic brain injury (TBI) is an extremely complex condition due to heterogeneity in mechanism, underlying conditions, and secondary injury. Pre-clinical clinical researchers face challenges with reproducibility that negatively impact translation therapeutic development for improved TBI patient outcomes. To address this challenge, Working Groups expanded upon previous efforts developed common data elements (CDEs) describe the most frequently used experimental parameters. The working groups...
Impairments in social behavior characterize many neurodevelopmental psychiatric disorders. In fact, the temporal emergence and trajectory of these deficits can define disorder, specify their treatment signal prognosis. The sophistication mouse models with neurobiological endophenotypes aspects diseases has increased recent years, necessity to evaluate models. We adapted an assay for multimodal characterization at different development time points (juvenile, adolescent adult) control mice...
Phenotyping mouse model systems of human disease has proven to be a difficult task, with frequent poor inter- and intra-laboratory replicability, particularly in behavioral domains such as social cognitive function. However, establishing robust animal strong construct validity is fundamental importance they are central tools for understanding pathophysiology developing therapeutics. To complete our studies relevant autism spectrum disorder (ASD), we present replication the main findings from...
We examined the neural substrates of fear memory formation and maintenance when repeated recall was used to prevent forgetting in young animals. In contrast adult rats, juveniles failed show contextual responses at 4 d post-fear conditioning. Reconsolidation sessions 3 6 after conditioning restored 7 initial training. that received reconsolidation sessions, protein kinase M zeta (PKMζ) increased amygdala, but not hippocampus. These data suggest reminders PKMζ maintain juvenile animals...
This chapter explores existing data reproducibility and robustness initiatives from a cross-section of large funding organizations, granting agencies, policy makers, journals, publishers with the goal understanding areas overlap potential gaps in recommendations requirements. Indeed, vigorous stakeholder efforts to identify address irreproducibility have resulted development multitude guidelines but little harmonization. likely results confusion for scientific community may pose barrier...
These guidelines follow the recommendation of a number external bodies to regulate use animals in research. They can be used both for transparency publication, and this sense they extend what is being requested by journals, or regulatory funding institutions, request information prior, during, after funding, ensure adherence regulations. This checklist focuses on rodents Other species (such as marine mammals, primates, invertebrates) will covered future separated checklists. based extends...
Abstract To expand, analyze and extend published behavioral phenotypes relevant to autism spectrum disorder (ASD), we present a study of three ASD genetic mouse models: Feng’s Shank3 tm2Gfng model, hereafter Shank3/F , Jiang’s tm1Yhj Shank3/J the Cacna1c deletion model. The models mimick gene mutations associated with Phelan-Mcdermid syndrome model recapitulates underlying Timothy syndrome. current utilizes both standard novel, computer-vision based tests, same methdology used in our...
While high risk of failure is an inherent part developing innovative therapies, it can be reduced by adherence to evidence-based rigorous research practices. Numerous analyses conducted date have clearly identified measures that need taken improve rigor. Supported through the European Union’s Innovative Medicines Initiative, EQIPD consortium has developed a novel preclinical quality system applied in both public and private sectors free for anyone use. The Quality System was designed suited...
3. Abstract Background Phenotyping mouse model systems of human disease has proven to be a difficult task, with frequent poor inter- and intra-laboratory replicability translatability, particularly in behavioral domains such as social verbal function. However, establishing robust animal strong construct validity is fundamental importance they are central tools for understanding pathophysiology developing therapeutics. To complete our studies relevant autism spectrum disorder (ASD), we...
Neurofibrillary tangles with accelerated amyloidosis and plaque formation are widely thought to play a major role in development of Alzheimer's disease pathology. The rTg4510 mouse, model tauopathy, overexpresses P301L mutant human Tau the forebrain. APP/PS1 transgenic mouse mutated forms genes for amyloid precursor protein (APPsw) presenilin 1 (m146L) is used study deposition. Generally, these lines mice exhibit an age-dependent region-specific progression neuropathology. Additionally,...
Neurofibrillary tangles (NFT) are the second hallmark of Alzheimer’s disease (AD) and well correlated to disturbances in cognition. The rTg4510 mouse, a model tauopathy, overexpresses P301L mutant human Tau forebrain under control tetracycline tans-acting element (TET-Off). mice develop neurobrillary pathology already at an age 2,5 3 months, show progressive gross brain atrophy significant neuronal loss hippocampal structures. study investigated effects suppression transgene expression by...
Tg2576 mice are one of the standard models for AD research. The APP/PS1 produced by cross-breeding them with PS1 tg mice. mutation changes processing APP, increasing proportion pro-aggregatory ABeta42, accelerating formation plaque pathology and functional deficits. It is first time that both were compared using proprietary, sensitive behavioral tests may have capability to increase predictive drug testing in animals models. was interest explore what extent differences performance between...