A5108031855- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- MicroRNA in disease regulation
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Block Copolymer Self-Assembly
- Bone and Dental Protein Studies
- Chromatin Remodeling and Cancer
- Intestinal Malrotation and Obstruction Disorders
- Tannin, Tannase and Anticancer Activities
- Cancer Research and Treatments
- Infant Nutrition and Health
- Cancer Immunotherapy and Biomarkers
- Fatty Acid Research and Health
- T-cell and B-cell Immunology
- Glaucoma and retinal disorders
- Congenital Anomalies and Fetal Surgery
- Cancer Mechanisms and Therapy
- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Metabolomics and Mass Spectrometry Studies
Affiliated Eye Hospital of Wenzhou Medical College
2019
Wenzhou Medical University
2019
Markey Cancer Center
2018
University of Kentucky
2018
Dana-Farber Cancer Institute
2018
Lemuel Shattuck Hospital
2018
Harvard University
2018
Johns Hopkins University
2012-2017
Johns Hopkins Medicine
2012-2014
SNF'ing out antitumor immunity Immune checkpoint inhibitors induce durable tumor regressions in some, but not all, cancer patients. Understanding the mechanisms that determine sensitivity to these drugs could potentially expand number of patients who benefit (see Perspective by Ghorani and Quezada). Pan et al. discovered cells which a specific SWI/SNF chromatin remodeling complex had been experimentally inactivated were more sensitive T cell–mediated killing. The responsive interferon-γ,...
MICA and MICB are expressed by many human cancers as a result of cellular stress, can tag cells for elimination cytotoxic lymphocytes through natural killer group 2D (NKG2D) receptor activation. However, tumors evade this immune recognition pathway proteolytic shedding proteins. We rationally designed antibodies targeting the α3 domain, site shedding, found that these prevented loss cell surface cancer cells. These inhibited tumor growth in multiple fully immunocompetent mouse models reduced...
DNA-containing micellar nanoparticles with distinctly different and highly uniform morphologies are prepared via condensation of plasmid DNA a block copolymer polyethylene glycol polycation in solvents polarity. Molecular dynamics simulations explain the underlying mechanism. As service to our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be re-organized for online delivery, but not copy-edited or typeset. Technical...
The effective elongation of the vertebrate body axis depends on precise coordination cell movements and fate determination within posterior tailbud region. However, specific biological determinant that integrates these cellular processes during tail bud has remained unclear. Here, we demonstrate spatiotemporal heterogeneity chromatin condensation states governs both motility mesodermal progenitor zone (MPZ) presomitic mesoderm (PSM) zebrafish elongation. By integrating experiments with...
Purpose: To objectively quantify cone density (CD) and microvascular (MVD) in normal subjects patients with moderate or severe retinitis pigmentosa (RP) by adaptive optics (AO) optical coherence tomography angiography (OCTA) to evaluate the changes parafoveal regions. Method: Thirty-seven eyes from 20 RP 54 29 age-matched healthy participants underwent AO fundus OCTA imaging. images covering a 3-mm-diameter area centered on fovea were subdivided into 5 equidistant concentric rings (C1–C5)....
PEGylated polycation/DNA micellar nanoparticles have been developed that can undergo shape transformation upon cleavage of the PEG grafts in response to an environmental cue. As a proof-of-principle, DNA with higher grafting density adopting long, worm- and rod-like morphologies, transition more condensed spherical short-rod morphologies fraction from copolymer. This leads increased surface charges, correlating improved transfection efficiency.
Efficient delivery of short interfering RNA (siRNA) remains one the primary challenges interference therapy. Polyethylene glycol (PEG)ylated polycationic carriers have been widely used for condensation DNA and molecules into complex-core micelles. The PEG corona such nanoparticles can significantly improve their colloidal stability in serum, but PEGylation also reduces capacity, hindering generation micellar particles with sufficient complex stability. This presents a particularly...
Micellar nanoparticles are created by Hai-Quan Mao, Erik Luijten, and co-workers on page 227 through complexation of plasmid DNA (green) block copolymers (blue/brown). Variation solvent polarity allows systematic control over the micelle shape (modeling images shown in foreground image transmission electron microscopy background) without changing their chemical composition, paving way for targeted gene delivery.
Objective To explore the diagnosis, treatments and operative timing of neonatal necrotizing enterocolitis (NEC). Methods Retrospective analyses were conducted for clinical data 164 children NEC. Results Among 72 surgical cases, 17 (23.61%) cases had an automatic discharge because extensive bowel necrosis; among 41 (56.94%) intestinal colostomy, 6/41(14.63%) discharge. Because too short proximal 14 underwent direct anastomosis 5/14 (35.71%) Among 92 conservatively treated 11/92...