A5022184232- Vehicle emissions and performance
- Neurogenesis and neuroplasticity mechanisms
- Lysosomal Storage Disorders Research
- Neuroscience and Neuropharmacology Research
- Soil erosion and sediment transport
- Landslides and related hazards
- Advanced Combustion Engine Technologies
- Ion channel regulation and function
- Axon Guidance and Neuronal Signaling
- Developmental Biology and Gene Regulation
- Engineering Applied Research
- Erythrocyte Function and Pathophysiology
- Trypanosoma species research and implications
- Pluripotent Stem Cells Research
- Oral microbiology and periodontitis research
- Vehicle Dynamics and Control Systems
- Porphyrin and Phthalocyanine Chemistry
- Air Quality Monitoring and Forecasting
- Engine and Fuel Emissions
- S100 Proteins and Annexins
- Electric and Hybrid Vehicle Technologies
- Humic Substances and Bio-Organic Studies
- Effects of Radiation Exposure
- Microbial Inactivation Methods
- Retinal Development and Disorders
Meiji Pharmaceutical University
2013-2024
Forestry and Forest Products Research Institute
2002-2024
Nihon University
2020
University of Tsukuba Hospital
2020
GTx (United States)
2018
Kyoto University
2007-2016
Baylor College of Medicine
2008-2015
National Traffic Safety and Environment Laboratory
2006-2011
Horiba (Japan)
1992-2011
Nippon Koei (Japan)
2010
The axon initial segment (AIS) functions as both a physiological and physical bridge between somatodendritic axonal domains. Given its unique molecular composition, location, physiology, the AIS is thought to maintain neuronal polarity. To identify basis of this property, we used adenovirus-mediated RNA interference silence protein expression in polarized neurons. Some proteins are remarkably stable with half-lives at least 2 wk. However, silencing cytoskeletal scaffold ankyrinG (ankG)...
Action potential initiation and propagation requires clustered Na(+) (voltage-gated [Nav]) channels at axon initial segments (AIS) nodes of Ranvier. In addition to ion channels, these domains are characterized by cell adhesion molecules (CAMs; neurofascin-186 [NF-186] neuron glia-related CAM [NrCAM]), cytoskeletal proteins (ankyrinG betaIV spectrin), the extracellular chondroitin-sulfate proteoglycan brevican. Schwann cells initiate peripheral nervous system node formation clustering NF-186,...
High densities of ion channels at axon initial segments (AISs) and nodes Ranvier are required for initiation, propagation, modulation action potentials in axons. The organization these membrane domains depends on a specialized cytoskeleton consisting two submembranous cytoskeletal scaffolding proteins, ankyrinG (ankG) βIV spectrin. However, it is not known which proteins the principal organizer, or if mechanisms governing formation AIS also apply to nodes. We identify distinct protein domain...
Clustered Kv1 K + channels regulate neuronal excitability at juxtaparanodes of myelinated axons, axon initial segments, and cerebellar basket cell terminals (BCTs). These are part a larger protein complex that includes adhesion molecules scaffolding proteins. To identify proteins assembly, clustering, and/or maintenance axonal channel complexes, we immunoprecipitated Kv1.2 α subunits, then used mass spectrometry to interacting We found disintegrin metalloproteinase 22 (ADAM22) is component...
Paranodal junctions of myelinated nerve fibers are important for saltatory conduction and function as paracellular membrane protein diffusion barriers flanking nodes Ranvier. The formation these specialized axoglial contacts depends on the presence three cell adhesion molecules: neurofascin 155 glial a complex Caspr contactin axon. We isolated axonal membranes highly enriched in paranodal proteins then used mass spectrometry to identify additional associated with junction. This strategy led...
Postsynaptic density-93 (PSD-93)/Chapsyn-110 is a PDZ (PSD-95/Discs large/zona occludens-1) domain-containing membrane-associated guanylate kinase (MAGUK) that functions as scaffold to assemble channels, receptors, and other signaling proteins at cell membranes. PSD-93 highly enriched synapses, but mice lacking this protein have no synaptic structural abnormalities, probably because of overlapping expression redundancy with MAGUKs. Consequently, the function not well understood. Here, we...
Understanding the molecular and physiological determinants of cortical neuronal progenitor cells is essential for understanding development human brain in health disease. We used surface marker fucose N -acetyl lactosamine (LeX) (also known as CD15) to isolate from ventricular/subventricular zone fetal at second trimester gestation study their progeny vitro . LeX + had typical bipolar morphology, radial orientation, antigen profiles, characterizing them a subtype glia (RG) cells. Four...
ABSTRACT Recently, it has been suggested that the oral administration of Porphyromonas gingivalis, a keystone pathogen for periodontal disease, induces dysbiosis mouse intestinal microbiota and affects barrier function. Since streptococci are predominant bacterial group, we compared effect their on tract to P. gingivalis. Swallowing bacteria caused gut dysbiosis, due increased Bacteroides Staphylococcus decreased Lactobacillus spp. Furthermore, infection an increase in lactate decreases...
During peripheral nerve development, Schwann cells ensheathe axons and form myelin to enable rapid efficient action potential propagation. Although myelination requires profound changes in cell shape, how neuron–glia interactions converge on the cytoskeleton induce these is unknown. Here, we demonstrate that submembranous cytoskeletal proteins αII βII spectrin are polarized colocalize with signaling molecules known modulate vitro. Silencing expression of spectrins inhibited vitro,...
Abstract We discovered an epoch‐making gene transfer method utilizing discharge plasma. Although electroporation is commonly used in present experiments, it cannot genes into primary cells sufficiently. The atmospheric pressure plasma employed this study was originally for surface treatment of non‐biological materials. hypothesized that could provide a suitable effect on the target and applied to various types cells. technology succeeded efficient green fluorescence protein (GFP) plasmid...
To characterize the functional differentiation of neural stem cells into smooth muscle cells, multipotent in central nervous system (CNS) were isolated from rat embryonic day 14 (E14) cortex and cultured by neurosphere formation serum‐free medium presence 10 ng ml −1 basic fibroblast growth factor. Differentiation was induced addition % fetal bovine serum to low‐density cultures (2.5 × 3 cm −2 ). Immunological analyses reverse transcriptase‐polymerase chain reaction indicated that...
In myelinated axons, K + channels are clustered in distinct membrane domains to regulate action potentials (APs). At nodes of Ranvier, Kv7 expressed with Na channels, whereas Kv1 flank at juxtaparanodes. Regulation axonal APs by would be particularly important fast-spiking projection neurons such as cerebellar Purkinje cells. Here, we show that BK/Slo1 the paranodal junctions cell axons rat and mouse. The junction is formed a set cell-adhesion molecules, including Caspr, between node...
Drosophila glial cells missing (gcm) is a key gene that determines the fate of stem within nervous system. Two mouse gcm homologs have been identified, but their function in system remains to be elucidated. To investigate function, we constructed retroviral vectors harboring and two Gcm genes. Expression these genes appeared influence fibroblast features. In particular, Gcm1 induced expression astrocyte-specific Ca(2+)-binding protein, S100beta, those cells. Introduction cultured from...
The central nervous system of the mammalian embryo is organized according to expression region-specific transcription factors along anteroposterior and/or dorsoventral axis. For example, dorsal ventricular zone (VZ) embryonic spinal cord expresses Pax3 and Pax7, ventral VZ Pax6, more Nkx2.2. Properties neuronal precursors located in are determined by characteristic patterns these factors, leading generation distinct classes neurons. Recent studies demonstrated that radial glial cells produce...
Abstract Langmuir‐Blodgett films of a cellulose derivative containing porphyrins, porphyrin‐cellulose, were fabricated in order to construct cellulose‐based molecular photocurrent generation system. On visible light illumination the LB monolayer film deposited on an ITO electrode, anodic photocurrents observed with quantum yield 1.6% at applied potential 0 V versus SCE, and 3.8–4.6% 0.2–0.3 SCE. These values indicate that self‐quenching photoexcited porphyrins was suppressed, while porphyrin...
Arrestins are known to be involved not only in the desensitization and internalization of G protein-coupled receptors but also protein-independent activation mitogen-activated protein (MAP) kinases, such as extracellular signal-regulated kinase (ERK) c-Jun N-terminal (JNK), regulate cell proliferation inflammation. Our previous study revealed that histamine H1 receptor-mediated ERK is dually regulated by Gq proteins arrestins. In this study, we investigated roles arrestins JNK Chinese...
Abstract Neuron–glia interactions at paranodal junctions play important roles in action potential propagation. Among their many functions, they contribute to the passive electrical properties of myelinated nerve fibers and actively regulate polarized distribution ion channels along axons. Despite importance, relatively little is known about molecules responsible for paranode formation function. Paranodal junction apparently depends on among three cell adhesion molecules: caspr contactin axon...
Abstract Sandhoff disease (SD) is caused by the loss of β-hexosaminidase (Hex) enzymatic activity in lysosomes resulting from Hexb mutations. In SD patients, Hex substrate GM2 ganglioside accumulates abnormally neuronal cells, loss, microglial activation, and astrogliosis. −/− mice, which manifest a phenotype similar to SD, serve as animal models for examining pathophysiology SD. mice reach ~8 weeks without obvious neurological defects; however, trembling begins at 12 accompanied startle...