A5015005847- Silk-based biomaterials and applications
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Nanoplatforms for cancer theranostics
- Antimicrobial Peptides and Activities
- Polymer Surface Interaction Studies
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Protein Hydrolysis and Bioactive Peptides
- Gold and Silver Nanoparticles Synthesis and Applications
- Extracellular vesicles in disease
- Advanced Nanomaterials in Catalysis
- Advanced biosensing and bioanalysis techniques
- Calcium Carbonate Crystallization and Inhibition
- Collagen: Extraction and Characterization
- Surface Modification and Superhydrophobicity
- Biochemical and Structural Characterization
- Tissue Engineering and Regenerative Medicine
- Biosensors and Analytical Detection
- Periodontal Regeneration and Treatments
- Advanced Sensor and Energy Harvesting Materials
- Skin Protection and Aging
- Bone Metabolism and Diseases
- Advanced Materials and Mechanics
- Bee Products Chemical Analysis
Zhejiang University
2015-2025
Chinese University of Hong Kong
2024
Sir Run Run Shaw Hospital
2024
University of Oklahoma
2017-2019
Hangzhou Xixi hospital
2019
Hangzhou Academy of Agricultural Sciences
2015-2017
Kerala Veterinary and Animal Sciences University
2015
Biomacromolecules have been used as templates to grow hydroxyapatite crystals (HAps) by biomineralization fabricate mineralized materials for potential application in bone tissue engineering. Silk sericin is a protein with features desirable biomaterial, such increased hydrophilicity and biodegradation. Mineralization of the silk from Antheraea pernyi (A. pernyi) silkworm has rarely reported. Here, first time, nucleation HAps on A. (AS) was attempted through wet precipitation method...
Abstract Brain damage is a common tissue caused by trauma or diseases, which can be life-threatening. Stem cell implantation an emerging strategy treating brain damage. The stem commonly embedded in matrix material for implantation, protects and induces differentiation. Cell differentiation induction this decisive the effectiveness of treatment strategy. In work, we present injectable fibroin/MXene conductive hydrogel as carrier, further enables in-vivo electrical stimulation upon cells...
Abstract Development of stimulus‐responsive materials is crucial for novel soft actuators. Among these actuators, the moisture‐responsive actuators are known their accessibility, eco‐friendliness, and robust regenerative attributes. A major challenge (MRSAs) achieving significant bending curvature within short response times. Many plants naturally perform large deformation through a layered hierarchical structure in to moisture stimuli. Drawing inspiration from bionic Delosperma nakurense...
Bombyx mori (B. mori) silk sericin is a protein with features desirable as biomaterial, such increased hydrophilicity and biodegradation, well resistance to oxidation, bacteria, ultraviolet light. In contrast other widely studied B. proteins fibroin, still unexplored building block for fabricating thus facile technique of processing it into material needed. Here, electrospinning technology was used fabricate biomaterials from two forms different molecular weights, one low (12.0 kDa) (LS)...
Ca<sup>2+</sup>binding induces self-assembly of sericin into a nanofibrous network and the subsequent mineralization for promoting osteogenic differentiation BMSCs.
Bombyx mori silk fibroin (SF) is a promising natural biocompatible protein. However, its interaction with graphene oxide (GO) has never been studied and the resultant SF/GO matrix not used to direct stem cell fate. Herein, we found out that mixing SF molecules GO nanosheets in an aqueous solution can trigger assembly of nanoparticles into oriented nanofibrils due guidance nanosheets, forming films unique nanotopographies improved modulus upon removal solvent. When mass percentage 2 10%,...
Peptides can introduce new functions to biomaterials but their immobilization usually relies on inefficient physical adsorption or tedious chemical conjugation. Using the Bombyx mori silk fibroin (SF) membrane (SFm) as a model biomaterial, here, we demonstrate universal strategy for discovering peptides that "stick" biomaterial functionalize it. Specifically, two peptide motifs, one screened by phage display biopanning binding (i.e., SF) and another derived from an osteogenic growth factor...
Sunscreens play a crucial role in protecting the skin from ultraviolet (UV) damage. However, present commercial sunscreens have tendency to generate free radicals UV window, resulting serious inflammatory responses and health problems. In this study, we demonstrate that silk fibroin microspheres (SFMPs) assembled regenerated (SF) could scavenge while preventing irradiation thus promising sunscreen. The SFMP reflected more light than SF presented higher stability of organic sunscreens. vitro...
Abstract Assembling natural proteins into large, strong, bone‐mimetic scaffolds for repairing bone defects in large‐animal load‐bearing sites remain elusive. Here this challenge is tackled by assembling pure silk fibroin (SF) 3D with cortical‐bone‐like lamellae, superior strength, and biodegradability through freeze‐casting. The unique lamellae promote the attachment, migration, proliferation of tissue‐regenerative cells (e.g., mesenchymal stem [MSCs] human umbilical vein endothelial cells)...
Abstract Little is known about the role of biocompatible protein nanoridges in directing stem cell fate and tissue regeneration due to difficulty forming nanoridges. Here an ice‐templating approach proposed produce semi‐parallel pure silk The key this that water droplets formed films are frozen into ice crystals (removed later by sublimation), pushing surrounding molecules be assembled Unlike flat films, unique can induce differentiation human mesenchymal cells (MSCs) osteoblasts without any...
Nanodendrite particles (NDs) with densely branched structures and biomimetic architectures have exhibited great promise in tumor therapy owing to their prolonged vivo circulation time exceptional photothermal efficiency. Nevertheless, traditional NDs are deficient terms of specific surface modification targeting tumors, which restrict potential for broader clinical applications. Here, we developed coronavirus-like gold through a seed-mediated approach using silk fibroin (SF) as capping...
Abstract The improper and inadequate treatment of industrial, agricultural, household wastewater exerts substantial pressure on the existing ecosystem poses a serious threat to health both humans animals. To address these issues, different types materials have been employed eradicate detrimental pollutants from facilitate reuse water resources. Nevertheless, owing challenges associated with degradation traditional post‐use their incompatibility environment, natural biopolymers garnered...
Uncontrolled deep bleeding, commonly encountered in surgical procedures, combat injuries, and trauma, poses a significant threat to patient survival recovery. The development of effective hemostatic agents capable precisely targeting trauma sites tissues rapidly halt bleeding remains considerable challenge. Drawing inspiration from the natural cascade, we present platelet-like microspheres composed silk fibroin (SF) thrombus-targeting peptides, engineered mimic platelets for rapid hemostasis...
Abstract Bombyx mori silk fibroin (SF) is known to be capable of facilitating nucleation the hydroxyapatite crystals (HAps). To find out how SF mediates HAps, self‐assembly in 1.5 simulated body fluid (SBF) was observed this study through design a co‐solution and 1.5‐times SBF (SF/1.5 SBF). After SF/1.5 incubated at 37.2 °C up 7 days, SEM, X‐ray, Fourier transform infrared (FTIR) observations indicated that HAps increased. In addition, structure transited from random coil into β‐sheet by...
In order to use a simple and ecofriendly method prepare porous silk scaffolds, aqueous fibroin solution (ASF) was extracted from gland of 7-day-old fifth instar larvae Bombyx mori (B. mori). SDS-page analysis indicated that the obtained had molecular weight higher than 200 kDa. The fabrication scaffolds ASF achieved by using freeze-drying method. pore is homogenous tends become smaller with an increase in concentration ASF. Conversely, porosity decreased. show impressive compressive strength...
Abstract Silk fibre mechanical properties are attributed to the development of a multi-scale hierarchical structure during spinning. By careful ex vivo processing B. mori silkworm silk solution we arrest spinning process, freezing-in mesoscale structures corresponding three distinctive stages; gelation, fibrilization and consolidation phase identified in this work, process highlighted by emergence extinction ‘water pockets’. These transient water pockets manifestation interplay between...
Abstract The use of biomacromolecules as templates to control the nucleation and growth hydroxyapatite crystals prepare bioactive materials is a valuable approach in bone tissue engineering. Here, an artificial periosteum prepared by biomineralizing Antheraea pernyi fibroin ( AF ) membrane with prenucleated nanoclusters, which can promote osteogenic differentiation mesenchymal stem cells (MSCs) induce formation matrix protein vivo. To achieve this, biologically inspired calcium phosphorus...
This silk fibroin/graphene oxide-based scaffold with nano-fibrillar and micro-lamellar structures can effectively promote cell migration, adhesion, proliferation, facilitating the differentiation of neural stem cells into neurons.