A5089724313- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Heat shock proteins research
- DNA and Nucleic Acid Chemistry
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Protist diversity and phylogeny
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurosurgical Procedures and Complications
- Epigenetics and DNA Methylation
- Testicular diseases and treatments
- Cancer-related gene regulation
- Genetic and Kidney Cyst Diseases
- RNA modifications and cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Sichuan University
2022-2024
University of Hong Kong
2023-2024
West China Second University Hospital of Sichuan University
2024
Centrosomal protein dysfunction might cause ciliopathies. However, the role of centrosomal proteins in male infertility remains poorly defined. Here, we identified a pathogenic splicing mutation CEP78 infertile patients with severely reduced sperm number and motility, typical multiple morphological abnormalities flagella phenotype. We further created Cep78 knockout mice, which showed an extremely low count, completely aberrant morphology, approximately null motility. The mice could not be...
Meiosis is a specialized cell division process that generates gametes for sexual reproduction. However, the factors and underlying mechanisms involving meiotic progression remain largely unknown, especially in humans. Here, it first showed HSF5 associated with human spermatogenesis. Patients pathogenic variant of are completely infertile. Testicular histologic findings patients reveal rare postmeiotic germ cells resulting from prophase I arrest. Hsf5 knockout (KO) mice confirms loss causes...
To monitor the levels of mitochondrial DNA G-quadruplexes (mtDNA G4s) in spermatozoa and to explore possibility using mtDNA G4s as a reliable marker patients with multiple clinical insemination failures, novel chemical TPE-mTO probe engineered our previous work was used on both samples from mice sperm fertilization failure. Expression valosin-containing protein zona-free hamster egg assay were evaluate mitophagy human penetration. RNA-sequencing expression changes key genes affected by G4s....
Infertility affects an estimated 10 to 15 percent of couples worldwide, with approximately half the cases attributed male-related issues. Most men diagnosed infertility exhibit symptoms such as oligospermia, asthenospermia, azoospermia, and compromised sperm quality. Spermatogenesis is a complex tightly coordinated process germ cell differentiation, precisely regulated at transcriptional, posttranscriptional, translational levels ensure stage-specific gene expression during development...
The ubiquitin ligase HECT, UBA, and WWE domain-containing E3 protein 1 is essential for the establishment maintenance of spermatogonia. However, role in regulating germ cell differentiation remains unclear, clinical evidence linking to male infertility pathogenesis lacking.