A5076850237- Advanced biosensing and bioanalysis techniques
- Molecular Junctions and Nanostructures
- Electrochemical sensors and biosensors
- Hormonal Regulation and Hypertension
- Glycosylation and Glycoproteins Research
- Electrochemical Analysis and Applications
- Monoclonal and Polyclonal Antibodies Research
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Peroxisome Proliferator-Activated Receptors
- Immune Cell Function and Interaction
- Blood Pressure and Hypertension Studies
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Inflammatory Bowel Disease
- RNA modifications and cancer
- Synthesis and Catalytic Reactions
- Renal Diseases and Glomerulopathies
- Eicosanoids and Hypertension Pharmacology
- Cellular Mechanics and Interactions
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
Hiroshima University Hospital
2023
Kure Kyosai Hospital
2022
Kyowa Kirin (Japan)
2022
National Institute of Advanced Industrial Science and Technology
2009-2020
Analysis Group (United States)
2018
Konica Minolta (Japan)
2018
Biomedical Research Institute
2018
Keio University
2003-2011
Hiroshima University
2006-2011
Kyoto University
2009
Sirt1, a NAD-dependent protein deacetylase, is reported to regulate intracellular metabolism and attenuate reactive oxidative species (ROS)-induced apoptosis leading longevity acute stress resistance. We created transgenic (TG) mice with kidney-specific overexpression of Sirt1 using the promoter sodium-phosphate cotransporter IIa (Npt2) driven specifically in proximal tubules investigated role protection against kidney injury (AKI). also elucidated number or function peroxisome mitochondria...
Dimethylarginie dimethylaminohydrolase (DDAH) degrades asymmetric dimethylarginine (ADMA), an endogenous nitric oxide (NO) synthase inhibitor, and comprises 2 isoforms, DDAH1 DDAH2. To investigate the in vivo role of DDAH2, we generated transgenic mice overexpressing The manifested reductions plasma ADMA elevations cardiac NO levels but no changes systemic blood pressure (SBP), compared with wild-type mice. When infused into for 4 weeks, elevated SBP caused marked medial thickening...
The development of obesity involves multiple mechanisms. Here, we identify adipocyte signaling through the guanosine triphosphatase Rho and its effector Rho-kinase as one such mechanism. Mice fed a high-fat diet (HFD) showed increased activity in adipose tissue compared to mice low-fat diet. Treatment with inhibitor fasudil attenuated weight gain insulin resistance on HFD. Transgenic overexpressing an adipocyte-specific, dominant-negative form RhoA (DN-RhoA TG mice) decreased adipocytes,...
Although peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have an antihypertensive effect in vivo, the precise mechanism has not been fully elucidated. We examined their effects on Rho/Rho kinase pathway, a key regulator of vascular tone. In cultured rat aortic smooth muscle cells (RASMC), Rho stimulated by angiotensin II was suppressed pretreatment with pioglitazone and troglitazone, these were explained inhibition translocation to cell membrane. evaluated role Vav,...
Rho-kinase plays an important role in hypertension and is reported to interfere with insulin signaling through serine phosphorylation of receptor substrate-1 (IRS-1) cultured vascular smooth muscle cells. We therefore examined the development resistance Zucker obese rats. In skeletal muscles aortic tissues rats, activation RhoA/Rho-kinase was observed. Long-term inhibition by 4 wk treatment fasudil (a inhibitor) not only reduced blood pressure but corrected glucose lipid metabolism,...
Dimethylarginie dimethylaminohydrolase (DDAH) is a degrading enzyme for asymmetrical dimethylarginine, an endogenous NO synthase inhibitor. The molecular mechanism DDAH-induced vascular endothelial growth factor (VEGF) expression was examined.Although the transfection of vectors 2 isoforms DDAH, DDAH1, or DDAH2 increased DDAH activity in bovine aortic cells and human umbilical vein cells, secretion VEGF were only DDAH2-transfected cells. Knocking down gene reduced production, overexpression...
Cardiovascular events are accelerated in chronic kidney disease (CKD). Although deranged nitric oxide (NO) pathways and asymmetric dimethylarginine (ADMA) cause endothelial dysfunction, no direct evidence for coronary artery dysfunction CKD has been documented. was induced male dogs by heminephrectomy (1/2Nx) or five-sixths nephrectomy (5/6Nx). After 4 wk, renal ablation reduced GFR (control 76 [54 to 85]; 1/2Nx 38 [29 47]; 5/6Nx 15 [12 46] ml/min) elevated plasma ADMA 1.88 [1.68 2.54]; 2.51...
<i>Background: </i>Sodium picosulphate (Picolax) is considered by most British surgeons as standard preparation for colonoscopy and elective surgery. Oral sodium phosphate may be better tolerated more efficient bowel preparation. <i>Methods:</i> A randomized trial was performed to compare oral (n = 76) with Picolax 77) colorectal parallel study patients 51) or 52). Patient acceptability measured seven symptoms a linear analogue score. Quality of graded the surgeon...
We have isolated spontaneous mutants of Salmonella typhimurium which can swim in the presence antifilament antibodies. The molecular masses flagellins from these were smaller than that (52 kDa) wild-type flagellin. Two produced smallest (42 and 41 selected, domain structures analyzed by trypsin digestion then subjected to amino acid sequencing. two deletions at Ala-204 Lys-292 Thr-183 Lys-279, respectively. These deleted parts belong outer (D3) flagellin, is believed be surface filament....
Surface modification of glassy carbon (GC) and highly oriented pyrolytic graphite (HOPG) was carried out with diazonium, amine, azide, olefin derivatives bearing ferrocene as an electroactive moiety. Features the modified surfaces were evaluated by surface concentrations immobilized molecule, blocking effect against redox reaction, observation using cyclic voltammetry electrochemical scanning tunneling microscope (EC-STM). The measurement molecule revealed following three aspects: (i)...
This paper reports a sequence-specific immunoassay chip for DNA methylation assessment by microfluidic-based surface plasmon resonance (SPR) detection. was achieved utilizing an affinity measurement involving the target, (methyl)cytosine, in single-base bulge region and anti-methylcytosine antibody microchannel, following hybridization with biotinylated bulge-inducing probe. The probe alters target cytosine looped-out state because of π–π stacking between flanking bases target. design is...
We examined whether aldosterone/Rho/Rho-kinase pathway contributed to obesity-associated nephropathy.C57BL/6J mice were fed a high fat or low diet, and on diet treated with mineralocorticoid receptor antagonist, eplerenone.The not only developed obesity, but also manifested renal histological changes, including glomerular hypercellularity increased mesangial matrix, which paralleled the increase in albuminuria. Furthermore, enhanced Rho-kinase activity was noted kidneys from diet-fed mice,...
Angiotensin II enhances the development of atherosclerotic lesion in which cellular proliferation and/or migration are critical steps. Although cyclin-dependent kinase inhibitor, p27, and Rho/Rho-kinase pathway have recently been implicated as factors regulating these events cooperatively, their role vivo has not fully elucidated. We evaluated contribution p27 Rho-kinase to angiotensin II-induced vascular injury using p27-deficient mice. Two-week (1500 ng/kg per minute SC) infusion elicited...
A hybrid functional biomolecular interface designed at a molecular size level is very effective capturing an analyte with high sensitivity even if the interaction weak, as when detecting proteins carbohydrate. We and processed protein (lectin) recognition taking following points into consideration: (1) height (molecular length) difference between spacer molecules; (2) ratio of molecules in interface. When maltoside part (Concanavalin (Con A) group) OH group terminated exceeded (>(CH2)6),...
Invariant natural killer T (iNKT) cells are considered innate-like lymphocytes, and regulate the immunity against inflammation tumorigenesis. However, impact of iNKT in inflammation-associated tumorigenesis remains unclear. In this study, we examined physiological role a mouse colitis-associated colorectal cancer model. C57BL/6 (B6) Jα18 NKT cell-deficient KO (KO) mice were used. Colitis-associated was induced by azoxymethane (AOM) dextran sodium sulfate (DSS). The resulting tumours...
The development of a biocatalytic electrode consisting cytochrome P450 (CYP) proteins would be key technology with which to establish simple drug metabolizing biosensors or screening devices for inhibitors. We have successfully detected the direct electron transfer (DET) from human CYP layer microsome adsorbed on bare indium tin oxide (ITO) film without any modification layers and applied it metabolism evaluation. compared electrocatalytic properties two ITO films different surface...
We have investigated whether GH treatment influences the expression of UCP1, 2 and 3 mRNA in a KK-Ay obese mouse model. mice (n = 10) C57Bl/6J control were injected subcutaneously with human (1.0 mg/kg/day 3.5 mg/kg/day) for 10 days, compared physical saline. The weighed significantly less (p < 0.01 : 1.0 mg/kg/day, p 0.05 had smaller inguinal subcutaneous perimetric white adipose tissue (WAT) pads mg/kg/day), but increased skeletal muscle weight 0.05). brown (BAT) did not change...
To investigate the role of local colonic mucosal NK receptor-positive T (NKR(+) T) cells in regulation intestinal inflammation, we analyzed population and function these ulcerative colitis (UC).Colonic tissues were obtained from colonoscopic biopsies descending colon 96 patients with UC (51 endoscopically uninflamed, 45 inflamed) 18 normal controls. Endoscopic appearance histologic score at biopsied site determined by Matts' classification. A single cell suspension was prepared each biopsy...
Summary Background The increasing incidence of older‐onset ulcerative colitis (UC), which has a higher risk surgery, is global health issue. However, data regarding intravenous steroid treatment, one the important treatment options to avoid for UC lacking. Aims To evaluate association between onset age and effectiveness steroids in UC. Methods This retrospective multicentre (27 facilities) cohort study included moderate‐to‐severe hospitalised patients who underwent their first April 2014...