This paper describes a gradient-generating microfluidic platform for optimizing proliferation and differentiation of neural stem cells (NSCs) in culture. Microfluidic technology has great potential to improve cell (SC) cultures, whose promise cell–based therapies is limited by the inability precisely control their behavior Compared traditional culture tools, platforms should provide much greater over microenvironment rapid optimization media composition using relatively small numbers cells....

The visible green and red upconversion emissions in Er3+/Yb3+ doped β-NaGdF4 nanoparticles were enhanced by tridoping with Fe3+ ions (0–40 mol%). XRD, XPS, ICP-AES EDS data demonstrated successful incorporation of NaGdF4:Yb3+/Er3+ nanoparticles. effect on the luminescence NPs was investigated detail. emission intensities 34 30 times, respectively. maximum observed a sample containing mol% ions. A possible mechanism for is proposed. In addition, layer silica coated onto surface UCNPs to...

This paper describes and characterizes a novel microfabricated neuronal culture device. device combines microfabrication, microfluidic, surface micropatterning techniques to create multicompartment culturing that can be used in number of neuroscience research applications. The is fabricated poly(dimethylsiloxane), PDMS, using soft lithography techniques. PDMS placed on tissue dish (polystyrene) or glass substrate, forming two compartments with volumes less than 2 μL each. These are separated...

This paper describes a simple plasma-based dry etching method that enables patterned cell culture inside microfluidic devices by allowing patterning, fluidic bonding and sterilization steps to be carried out in single step. was used pattern cell-adhesive non-adhesive areas on the glass polystyrene substrates. The substrate for selective attachment growth of human umbilical vein endothelial cells, MDA-MB-231 breast cancer NIH 3T3 mouse fibroblasts, primary rat cortical neurons. Finally, we...

This paper describes a microfluidic approach to generate dynamic temporal and spatial concentration gradients using single device. Compared previously described method that produced fixed gradient shape for each device, this combines simple "mixer module" with generating network control manipulate number of different shapes. The profile is determined by the configuration fluidic inputs as well design microchannel network. By controlling relative flow rates separate syringe pumps, resulting...

The layer-by-layer assembly of layered double-hydroxide (LDH) nanocrystals was successfully accomplished by surface grafting using organic acids as coupling agents. adsorbed on the crystalline sheet edges LDH also play a key role in allowing or blocking intercalation reaction multilayered nanocrystals, thereby making it possible to form thin layers organic-LDH nanocomposite architectures solid substrates.

In this study, we demonstrate a new perspective on in vitro assessment method for evaluating quantum dot (QD) toxicity by using microfluidics technology. A biomimetic approach, based the flow exposure condition, was applied order to characterize cytotoxic potential of QD. addition, outcomes obtained from condition were compared those static condition. An cell array system established that used an integrated multicompartmented microfluidic device develop sensitive QDs modified with...

Highly ordered Mg/Al-based layered double hydroxide (LDH) nanocrystals on Si substrates were successfully obtained by ultrasonic treatment in organic solvents. LDHs intercalated with aliphatic α,ω-dicarboxylate prepared through anion exchange solvothermal at around 100 °C. Binary solvent mixtures of alcohol and toluene prevent dissolution the LDH preserve crystal structure during anion-exchange reaction. Atomic force microscopy results present conservation lateral positions morphologies...

Hydrotalcite-like, layered double hydroxide [Mg4Al2(OH)12]CO3·nH2O (LDH) nanocrystals immobilized on solid substrates were incorporated with anionic polymers as host matrixes in the intercalation of polyacrylates to produce polymer−inorganic hybrid nanocomposites films substrates. The novel functional prepared through a direct ion-exchange reaction poly(acrylic acid) (PAA) macromolecules into LDH binary solvent systems at 120 °C. SEM images reveal that designed mixing such alcohol and...

This chapter describes the fabrication and use microfluidic chambers for cell migration neuroscience research. Both are made using soft lithography replica molding. The main advantages of to create reproducibility, ease use, straightforward procedures. devices can be fabricated in biology chemistry laboratories with minimal access clean-room facilities. First, a chemotaxis chamber, which has been used investigating neutrophils, human breast cancer cells, other types, is described. Precise...

A synthetic layered double hydroxide (LDH: Mg-Al-CO3) was heated to 560 °C for 3 h in air, and the resulting calcined LDH (1.00 g dm−3) used sorption study of aquatic CrO4 2− (concentration range: 1.0 × 10−3 − 5.0 mol under CO2 free atmosphere. Reconstructed containing intercalated between interlayers formed this confirmed by X-ray powder diffraction (XRD), 27Al MAS NMR, FT-IR UV/VIS spectroscopies. The resulted increased concentration, suggesting that ion-exchange based mechanism is...

A novel nanostructured hybrid PMMA/MgAl-LDH thin film enables a strong UV-blocking effect, up to 97% below 300 nm, and excellent visible transparency. The graft density of PMMA films can be controlled by adjusting the area coverage immobilized LDH monolayer with highly-orientated structure on oxide, metal, polymer substrates. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made...

Microfluidic systems have significant implications in the field of vitro cell-based assays since they may allow conventional to be conducted an automated and high-throughput fashion. In this study, we combined a simple microfluidic cells-on-chip system with morphology-based image cytometric analysis approach for assessment Cd(2+) induced apoptosis Chang liver cell line. A efficient situ monitoring method quantifying progress death event was developed is presented here. Reasonable agreement...