Introduction . Psoriasis is a T cell-mediated inflammatory disease in which pathogenesis helper (Th) lymphocytes (Th1, Th17, and Th22) play an important role. The aim of the study was to assess serum levels some cytokines involved Th17 Th22 responses psoriatic patients. Material Methods comprised 60 patients 30 healthy controls. In collected from controls, concentrations IL-6, IL-12, IL-17, IL-20, IL-22, IL-23 were examined with ELISA kits. Severity skin lesions assessed by means PASI, BSA,...

Angiogenesis has been shown to substantially contribute the progression of chronic lymphocytic leukemia (CLL). Neuropilin-1 (NRP1) represents a receptor for vascular endothelial growth factor (VEGF), which reported be overexpressed in several malignancies. In our study, we characterized mRNA levels VEGF receptors including NRP1 large cohort CLL patients (n = 114), additionally performed detailed characterization expression on B cells, plasmacytoid dendritic cells (PDCs) and regulatory T...

The aim of the study was to evaluate concentrations IL-17 in serum and plaque scales psoriatic patients. We analyzed their association with clinical activity disease body mass index (BMI). Demographic data, medical history, serum, scale from plaques for assessment were collected all participants. severity assessed PASI (Psoriasis Area Severity Index), BSA (Body Surface Area), PGA (Physician Global Assessment), NAPSI (Nail Psoriasis DLQI (Dermatology Quality Life Index) scores. Obesity...

CD (cluster of differentiation) 69 and CD25 are considered early late markers the activation lymphocytes, respectively. is a part IL-2 receptor present on surface immune non-immune cells, with high amounts activated lymphocytes regulatory T cells. CD69 expressed various types white blood including newly infiltrating tissues isolated from subjects chronic auto-inflammatory diseases, several subtypes memory cells Primarily, was to be an marker but, right now, data derived in vitro vivo studies...

Abstract: Objectives: To evaluate the efficacy and toxicity of cladribine (2‐chlorodeoxyadenosine, 2‐CdA), cytarabine (Ara‐C), granulocyte‐colony stimulating factor (G‐CSF) (CLAG) regimen in refractory acute myeloid leukemia (AML) multicenter phase II study. Methods: The induction chemotherapy consisted 2‐CdA 5 mg/m 2 , Ara‐C2 g/m G‐CSF. In case partial remission (PR), a second CLAG was administered. Patients complete (CR) received two consolidation courses based on HD Ara‐C, mitoxantrone or...

// Monika Podhorecka 1 , Aneta Goracy Agnieszka Szymczyk Malgorzata Kowal Blanca Ibanez Olga Jankowska-Lecka Arkadiusz Macheta Aleksandra Nowaczynska Elzbieta Drab-Urbanek Sylwia Chocholska Dariusz Jawniak and Marek Hus Department of Haematooncology Bone Marrow Transplantation, Medical University Lublin, Poland Correspondence to: Podhorecka, email: monika.podhorecka@onet.pl Keywords: chronic lymphocytic leukemia, cytokines, ibrutinib, T-cells, T regulatory cells Received: January 16, 2017...

BACKGROUND:Psoriasis is an autoimmune and autoinflammatory disorder that has a significant impact on patient quality of life. The aim the study was to assess immune profiles patients with psoriasis multiple cytokine analysis. MATERIAL AND METHODS:Fifty-two male psoriatic 24 healthy volunteers were recruited. Granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon gamma (IFN-gamma), interleukin (IL)-1 beta, IL-2, Il-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-13, IL-17A, IL-18,...

In this study, we attempted to assess the interactions of resveratrol, a natural compound present in various plant species, with purine analogues fludarabine and cladribine terms their effects on DNA damage apoptosis chronic lymphocytic leukemia (CLL) cells. The experiments were performed ex vivo using short-term cell cultures blood bone marrow cells from newly diagnosed untreated patients. We analyzed expression active caspase-3 BCL-2/BAX ratio as markers phosphorylated histone H2AX (γH2AX)...

Despite many therapeutic regimens introduced recently, chronic lymphocytic leukemia (CLL) is still an incurable disorder. Thus, there urgent need to discover novel, less toxic and more effective drugs for CLL patients. In this study, we attempted assess simvastatin, widely used as a cholesterol-lowering drug, both single agent in combination with purine analogs-fludarabine cladribine-in terms of its effect on apoptosis DNA damage cells. The experiments were done ex vivo short-term cell...

Trabeculectomy remains the most efficient method of lowering he IOP applied for treatment glaucoma refractory to pharmacological treatment. Cataract is concerned as frequent late complication trabeculectomy. The aim study was analyse effect phacoemulsification with posterior chamber lens implantation on morphology and function filtering bleb in patients after previous successful trabeculectomy.The retrospective included 122 eyes treated primary open angle glaucoma, 50 (study group) which, a...

Background . Psoriasis is an inflammatory disease in which joints involvement may be insidious and difficult to detect. Bone cartilage biomarkers helpful screening patients with psoriasis for psoriatic arthritis (PsA). Objectives To assess bone serum psoriasis. Methods The study was conducted 2014 included 61 30 healthy individuals. In both groups, the concentrations of soluble receptor activator nuclear factor‐ κ B ligand (sRANKL), oligomeric matrix protein (COMP), osteoprotegerin (OPG),...

Adipokines are regulatory molecules which act as mediators of the inflammatory, fibrotic and metabolic processes by interacting with immune system.We hypothesized that chemerin visfatin pro-inflammatory properties play a significant role in inflammation systemic sclerosis. To address this hypothesis, we determined serum levels SSc patients, compared control group defined correlations clinical laboratory parameters patients.The study included 48 Caucasian female patients 38 healthy subjects...

Given the recent findings on importance of CD38 signaling in pathogenesis B-cell chronic lymphocytic leukemia (B-CLL), we hypothesized that single nucleotide polymorphisms (SNP) gene may be related to B-CLL risk. We evaluated two potentially functional SNPs, intronic rs6449182 (184C>G) and missense rs1800561 (418C>T, Arg140Trp) hospital-based case-control studies (study A validation study B). Genotyping was done using PCR-based assays a total 460 Polish Caucasian patients with 503...

Psoriasis is a chronic inflammatory disease mediated by T cell immunity. Programmed death 1 (PD-1), coinhibitory receptor, plays an important role in immune regulation and maintaining peripheral tolerance. The aim of the study was to compare expression PD-1 on cells between psoriatic patients healthy controls. included 75 52 volunteers. percentages absolute numbers CD3 + , CD4 CD8 were analyzed using flow cytometry. significantly decreased comparison with control group. No significant...

Localized scleroderma is an autoimmune disease primarily affecting the skin. The cause of remains unexplained although environmental factors are implicated, which likely to be responsible for activation endothelium and subsequent inflammation leading excessive synthesis collagen extracellular matrix components.To determine concentrations interleukin (IL)-27, transforming growth factor (TGF)-β1, TGF-β2, IL-6, sIL-6R in patients with localized compared controls assess relations between their...

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Bartosińska J, Zakrzewska E, Purkot et al. Decreased blood CD4+PD-1+ and CD8+PD-1+ T cells in psoriatic patients with without arthritis. Advances Dermatology Allergology/Postępy Dermatologii i Alergologii. 2018;35(4):344-350. doi:10.5114/ada.2018.75609. APA Bartosińska, J., Zakrzewska, E., Purkot, Michalak-Stoma, A., Kowal, M., & Krasowska, D. (2018). Alergologii, 35(4), 344-350....

Metalloproteinases (MMPs) and cytokines have a great impact on the pathogenesis of psoriasis. Cytokines, as key mediators inflammation autoimmune processes, play crucial role in regulation MMP expression different cell types. Parallel, MMPs an influence cytokine production. This interaction was not well recognized psoriatic patients. Our study is aimed at assessing selected serum levels their correlations with We observed significantly elevated level pro-MMP-1 MMP-9 patients’ comparison to...

Abstract Systemic sclerosis (SSc) is a complex, heterogeneous connective tissue disease, characterized by fibrosis and ECM deposition in skin internal organs, autoimmunity, changes the microvasculature. Profiling of circulating miRNAs serum has been found to be changed pathological states, creating new possibilities for molecular diagnostics as blood-based biomarkers. This study was designed identify that are differentially expressed SSc might potentially contributing disease...

Localized scleroderma (LoSc) is a rare disease manifested by an inflammation and sclerosis of the skin. The latest studies focused on glycoprotein Krebs von den Lungen-6, surfactant protein-D, chemokine ligand 18 dipeptidylpeptidase 4 as potential biomarkers skin fibrosis in systemic scleroderma. Our study aimed to identify 6 miRNAs with elevated or decreased levels 38 LoSc patients (31 females, 7 males) compared healthy volunteers (HVs) correlate selected miRNAs' serum severity clinical...

Psoriasis (Ps) is an immune-mediated inflammatory skin disease that widely associated with the clinical features of metabolic syndrome (MetS), including hypertension, abdominal obesity, insulin resistance, type 2 diabetes and dyslipidemia. Osteopontin (OPN), a multifunctional protein involved in modulation processes, may contribute to development atherosclerosis MetS. Therefore, aim study was assessment correlation between OPN concentration peripheral blood presence MetS as well its...

Leptin and adiponectin have recently received the attention of researchers as attractive biomarkers in systemic sclerosis (SSc) because their role inflammatory process, vascular function fibrosis. We hypothesized that leptin may be associated with disease activity severity patients SSc.

Abstract Background: Zeta‐chain associated protein of 70kDa (ZAP‐70) is the most promising surrogate marker for immunoglobulin heavy chain variable region (IgV H ) mutation status in B‐cell chronic lymphocytic leukemia (B‐CLL). Crespo et al. proposed detection ZAP‐70 by flow cytometry. Recently several novel monoclonal antibodies appeared on market. Methods: We compared different staining methods utilizing (moAb) against ZAP‐70: anti‐ZAP‐70 PE, clone 1E7.2, 17A/P‐ZAP70 directly stained with...