A5103008554- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Extraction and Separation Processes
- CRISPR and Genetic Engineering
- Radioactive element chemistry and processing
- Nanoparticle-Based Drug Delivery
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Geochemistry and Elemental Analysis
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Response and Inflammation
- ATP Synthase and ATPases Research
- Chemical Synthesis and Characterization
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Folate and B Vitamins Research
- Nanoplatforms for cancer theranostics
- Animal Virus Infections Studies
- Drug Transport and Resistance Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
University of Science and Technology Beijing
2022-2024
Tongji University
2024
Shanghai University
2024
Shanghai Maritime University
2024
University of Pittsburgh
2023
University of Bristol
2023
Nanjing University of Information Science and Technology
2023
The Ohio State University
2016-2022
Sichuan University
2021
West China Hospital of Sichuan University
2021
Biodegradable nanomaterials effectively deliver long messenger RNAs in animal models.
SARS-CoV-2 has become a pandemic worldwide; therefore, an effective vaccine is urgently needed. Recently, messenger RNAs (mRNAs) have emerged as promising platform for vaccination. In this work, the untranslated regions (UTRs) of mRNAs are systematically engineered in order to enhance protein production. Through comprehensive analysis endogenous gene expression and de novo design UTRs, optimal combination 5' 3' UTR identified termed NASAR, which 5- 10-fold more efficient than tested UTRs....
Two-photon excitable (850 nm) NIR fluorescent turn-on (702 probe TP-NIR was synthesized for selective detection of cysteine (Cys). The itself shows is turned on by reaction with Cys in aqueous buffer. In addition, the displays greater selectivity over other thiols, including glutathione (GSH) and homocysteine (Hcy). Moreover, large Stokes shift, excitation, emission make this suitable biological imaging.
<bold>ClO1</bold>, a ratiometric fluorescent probe, effectively captured endogenous HClO in the lungs of lipopolysaccharide (LPS)-induced lung inflammation mouse model after intravenous administration.
Efficient and safe delivery of the CRISPR/Cas system is one key challenges for genome-editing applications in humans. Herein, we designed synthesized a series biodegradable lipidlike compounds containing ester groups mRNA-encoding Cas9. Two lead materials, termed N-methyl-1,3-propanediamine (MPA)-A MPA-Ab, showed tunable rate biodegradation. MPA-A with linear chains was degraded dramatically faster than MPA-Ab branched presence esterase or wild-type mice. Most importantly, demonstrated...
Abstract Antibodies targeting costimulatory receptors of T cells have been developed for the activation cell immunity in cancer immunotherapy. However, molecule expression is often lacking tumor-infiltrating immune cells, which can impede antibody-mediated Here, we hypothesize that delivery receptor mRNA to will enhance antitumor effects antibodies. We first design a library biomimetic nanoparticles and find phospholipid (PL1) effectively deliver (CD137 or OX40) cells. Then, demonstrate...
Abstract Toll‐like receptors (TLRs) and CD40‐related signaling pathways represent critical bridges between innate adaptive immune responses. Here, an immunotherapy regimen that enables co‐stimulation of TLR7/8‐ CD40‐mediated is developed. TLR7/8 agonist resiquimod (R848) derived amino lipids, RAL1 RAL2, are synthesized formulated into RAL‐derived lipid nanoparticles (RAL‐LNPs). The RAL2‐LNPs show efficient CD40 mRNA delivery to DCs both in vitro (90.8 ± 2.7%) vivo (61.3 16.4%). When combined...
The hot spot extraction agent N,N,N′,N′-tetraoctyl-3-oxoglutaramide (TODGA) has the advantages of novelty, high efficiency and environmental protection in separation rare earths by tandem extraction. In this paper, characteristic parameters for dysprosium, erbium, thulium ytterbium ions TOGDA–kerosene/hydrochloric acid system were calculated. Using a 10% TODGA–kerosene/5 mol·L−1 hydrochloric system, mixture containing Dy3+ 0.258 mol·L−1, Er3+ 0.252 Tm3+ 0.248 Yb3+ 0.242 can be separated...
// Cheng-Xiang Zhang 1 , Wei-Yu Zhao Lei Liu Rui-Jun Ju Li-Min Mu Yao Fan Zeng Hong-Jun Xie Yan Wan-Liang Lu Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, State Natural Biomimetic Drugs, School Pharmaceutical Sciences, Peking University, 100191, China Correspondence to: Lu, e-mail: luwl@bjmu.edu.cn Keywords: Functional targeting epirubicin liposomes, BBB, brain glioblastoma, neovasculatures, mechanism efficacy Received: April 14, 2015 Accepted: September Published:...
Previously, researchers discovered a series of anti-CRISPR proteins that inhibit CRISPR-Cas activity, such as Cas9 and Cpf1 (Cas12a). Herein, we constructed crRNA variants consisting chemically modified DNA-crRNA RNA-crRNA duplexes identified phosphorothioate (PS)-modified duplex completely blocked the function Cpf1. More important, without prehybridization, these PS-modified DNA oligonucleotides showed ability to suppress double-strand breaks induced by two orthologs, AsCpf1 LbCpf1....