A5086433168- Chronic Obstructive Pulmonary Disease (COPD) Research
- Long-Term Effects of COVID-19
- Respiratory Support and Mechanisms
- Tracheal and airway disorders
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- Atomic and Subatomic Physics Research
- Tumors and Oncological Cases
- Immune cells in cancer
- Cardiac Health and Mental Health
- Thermal Regulation in Medicine
- Enterobacteriaceae and Cronobacter Research
- Air Quality Monitoring and Forecasting
- Extracellular vesicles in disease
- Medical Imaging Techniques and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Inhalation and Respiratory Drug Delivery
- Advanced MRI Techniques and Applications
- Respiratory viral infections research
- Immune Cell Function and Interaction
St. Paul's Hospital
2021-2024
University of British Columbia
2021-2024
St. Paul's Hospital
2024
Background Long COVID impacts ∼10% of people diagnosed with coronavirus disease 2019 (COVID-19), yet the pathophysiology driving ongoing symptoms is poorly understood. We hypothesised that 129 Xe magnetic resonance imaging (MRI) could identify unique pulmonary phenotypic subgroups long COVID. Therefore, we evaluated ventilation and gas exchange measurements cluster analysis to generate imaging-based phenotypes. Methods COVID-negative controls participants who previously tested positive for...
Abstract To elucidate the important cellular and molecular drivers of pulmonary long COVID, we generated a single-cell transcriptomic map airway mucosa using bronchial brushings from patients with COVID who reported persistent symptoms. Adults without were recruited general community in greater Vancouver, Canada. The cohort was divided into those (PLC), which defined as persons new or worsening respiratory symptoms following at least one year their initial acute SARS-CoV-2 infection (N=9);...
To elucidate the important cellular and molecular drivers of pulmonary long COVID, we generated a single-cell transcriptomic map airway mucosa using bronchial brushings from patients with COVID who reported persistent symptoms. Adults without were recruited general community in Greater Vancouver, Canada. The cohort was divided into those (PLC), which defined as persons new or worsening respiratory symptoms following at least one year their initial acute SARS-CoV-2 infection (N=9); control...
Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to investigation pathobiology. Bronchial brushing, where mucosa sampled using cytological brush, viable, less method obtaining cells scRNA-seq. Here we describe development rapid...
Abstract An estimated 15% of patients are expected to continue exhibit respiratory symptoms after their SARS-CoV-2 infection. The mechanisms behind these remain unknown. We recruited twenty-three (65% female) who were on average 406 ± 251 days post-COVID-19. cohort consists primarily mild (non-hospitalized) (87%), with an age 43 15 years. Nine had self-reported dyspnea, which we defined as pulmonary long COVID (PLC). remaining 14 recovered without persistent dyspnea. performed a 38-panel...
Abstract Single-cell RNA-sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airways diseases. Currently, the majority of scRNA-seq studies in diseases have used invasive methods (airway biopsy, surgical resection) which carry inherent risks and thus present a major limitation to investigation airway pathology. Bronchial brushing, where mucosa sampled using cytological brush, viable, less method obtaining cells scRNA-seq. Here we are describing...
Many patients continue to suffer residual respiratory symptoms several months following a SARS-CoV-2 infection, known as "pulmonary long COVID" (PLC), but the mechanisms remain unknown. We performed single-cell RNA-sequencing on bronchoalveolar lavage (BAL) fluid characterize immune cell population in PLC relative healthy controls. BAL was obtained via bronchoscopy and washed twice with 0.4% bovine serum albumin Dulbecco's phosphate-buffered saline. Bioinformatic analysis Rsubread, SoupX,...