A5054596816- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Microbial Metabolic Engineering and Bioproduction
- Plant Gene Expression Analysis
- Polyamine Metabolism and Applications
- Advanced Photocatalysis Techniques
- Polysaccharides and Plant Cell Walls
- TiO2 Photocatalysis and Solar Cells
- Enzyme Structure and Function
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Plant Micronutrient Interactions and Effects
- Diet, Metabolism, and Disease
- Plant Molecular Biology Research
- Glycosylation and Glycoproteins Research
- Catalytic Processes in Materials Science
- Aldose Reductase and Taurine
- Advanced Nanomaterials in Catalysis
- Plant Stress Responses and Tolerance
- Peptidase Inhibition and Analysis
Cornell University
2021-2025
German Cancer Research Center
2024-2025
Ithaca College
2021-2025
Heidelberg University
2024-2025
Instituto de Agrobiotecnología del Litoral
2019-2022
Loyola University Chicago
2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2022
National University of the Littoral
2015-2022
Instituto de Desarrollo Tecnológico para la Industria Química
2015
Abstract The role of the RNA degradation product 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP) is poorly understood. Recent studies have identified 2′,3′-cAMP in plant material and determined its stress signaling. level increases upon wounding, dark, under heat, binding to an RNA-binding protein, Rbp47b, promotes granule (SG) assembly. To gain further mechanistic insights into function 2′,3′-cAMP, we used a multi-omics approach by combining transcriptomics, metabolomics, proteomics...
Abstract Diketopiperazines (DKPs) are chemically and functionally diverse cyclic dipeptides associated primarily with microbes. Few DKPs have been reported from plants animals; the best characterized is cyclo(His-Pro), found in mammalian central nervous system, where it arises proteolytic cleavage of a thyrotropin-releasing tripeptide hormone. Herein, we report identification cyclo(His-Pro) Arabidopsis (Arabidopsis thaliana), its levels increase upon abiotic stress conditions, including high...
Abstract A commercially available TiO 2 type (Kronos vlp 7000), doped with carbon and having an extended spectrum of light absorption wavelength, is investigated for photocatalytic degradation a representative air pollutant, namely acetaldehyde, under visible light. The modeling the studied system was carried out including proposal kinetic expressions contaminant generation main intermediates based on reaction mechanism, mass balances in reactor, lamps' superficial emission model to evaluate...
Abstract In our pursuit of understanding the protein-metabolite interactome, we introduced PROMIS, a co-fractionation mass spectrometry (CF-MS) technique focusing on biosynthetic and regulatory processes. However, challenge lies in distinguishing true interactors from coincidental co-elution when metabolite co-fractionates with numerous proteins. To address this, integrated two chromatographic techniques— size exclusion ion exchange—to enhance mapping interactions (PMIs) Escherichia coli ....
As autotrophic organisms, plants capture light energy to convert carbon dioxide into ATP, nicotinamide adenine dinucleotide phosphate (NADPH), and sugars, which are essential for the biosynthesis of building blocks, storage, growth. At night, metabolism growth can be sustained by mobilizing (C) reserves. In response changing environmental conditions, such as light-dark cycles, small-molecule regulation enzymatic activities is critical reprogramming cellular metabolism. We have recently...
We explored the ability of ADP-glucose pyrophosphorylase (ADP-Glc PPase) from different bacteria to use glucosamine (GlcN) metabolites as a substrate or allosteric effectors. The enzyme actinobacteria Kocuria rhizophila exhibited marked and distinctive sensitivity activation by GlcN-6P when producing ADP-Glc glucose-1-phosphate (Glc-1P) ATP. This behavior is also seen in Rhodococcus spp., only one known so far portray this activation. had more modest effect on other Actinobacteria (...
Sugar alcohols are major photosynthetic products in plant species from the Apiaceae and Plantaginaceae families. Mannose-6-phosphate reductase (Man6PRase) aldose-6-phosphate (Ald6PRase) key enzymes for synthesizing mannitol glucitol celery (Apium graveolens) peach (Prunus persica), respectively. In this work, we report first crystal structures of dimeric aldo/keto reductases (AKRs), Man6PRase (solved presence mannonic acid NADP+) Ald6PRase (obtained apo form). Both displayed typical TIM...
Most oxidoreductases that use NAD+ or NADP+ to transfer electrons in redox reactions display a strong preference for the cofactor. The catalytic efficiency of peach glucitol dehydrogenase (GolDHase) is 1800-fold higher than NADP+. Herein, we combined structural and kinetic data reverse cofactor specificity this enzyme. Using site-saturation mutagenesis, obtained D216A mutant, which uses both NADP+, although with different efficiencies (1000 ± 200 170 30 M–1 s–1, respectively). This mutant...
Abstract Raffinose (Raf) protects plant cells during seed desiccation and under different abiotic stress conditions. The biosynthesis of Raf starts with the production UDP-galactose by UDP-sugar pyrophosphorylase (USPPase) continues synthesis galactinol synthase (GolSase). Galactinol is then used to produce Raf. In this work, we report biochemical characterization USPPase ( Bdi USPPase) GolSase 1 GolSase1) from Brachypodium distachyon . catalytic efficiency was similar galactose 1-phosphate...
Abstract The role of the RNA degradation product 2’,3’-cyclic adenosine monophosphate (2’,3’-cAMP) is poorly understood. Recent studies have identified 2’,3’-cAMP in plant material and determined its stress signaling. level increases upon wounding, dark, heat, by binding to an RNA-binding protein, Rbp47b, promotes granule (SG) assembly. To gain further mechanistic insight into function, we used a multi-omics approach combining transcriptomics, metabolomics, proteomics dissect Arabidopsis...