A5044382039- Nanoparticle-Based Drug Delivery
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Porphyrin and Phthalocyanine Chemistry
- Advanced Drug Delivery Systems
- Lipid Membrane Structure and Behavior
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuroscience and Neural Engineering
- Conducting polymers and applications
- Nonlinear Optical Materials Studies
- Molecular Sensors and Ion Detection
- Liquid Crystal Research Advancements
- Crystallization and Solubility Studies
- Photodynamic Therapy Research Studies
- X-ray Diffraction in Crystallography
- Photochromic and Fluorescence Chemistry
- Surfactants and Colloidal Systems
- Polymer Surface Interaction Studies
- Molecular Communication and Nanonetworks
- Nitric Oxide and Endothelin Effects
- Hemoglobin structure and function
- Advanced Nanomaterials in Catalysis
United States Naval Research Laboratory
2016-2025
Naval Research Laboratory Center for Bio/Molecular Science and Engineering
2016-2024
University of Oklahoma Health Sciences Center
2013-2022
Dean McGee Eye Institute
2022
National Health Council
2017
National Academies of Sciences, Engineering, and Medicine
2017
Pusan National University
2008-2015
Shahjalal University of Science and Technology
2015
University of Central Oklahoma
2012
Liposomes are used as a delivery vehicle for drug molecules and imaging agents. The major impetus in their biomedical applications comes from the ability to prolong circulation half-life after administration. Conventional liposomes easily recognized by mononuclear phagocyte system rapidly cleared blood stream. Modification of liposomal surface with hydrophilic polymers delays elimination process endowing them stealth properties. In recent times, development various materials engineering...
We report the development of a quantum dot (QD)–peptide–fullerene (C60) electron transfer (ET)-based nanobioconjugate for visualization membrane potential in living cells. The bioconjugate is composed (1) central QD donor, (2) membrane-inserting peptidyl linker, and (3) C60 acceptor. photoexcited donor engages ET with acceptor, resulting quenching photoluminescence (PL) that tracks positively number moieties arrayed around QD. nature QD-capping ligand also modulates efficiency; neutral...
Melanins are macromolecules that ubiquitous in nature and impart a large variety of biological functions, including structure, coloration, radiation resistance, free radical scavenging, thermoregulation. Currently, the majority investigations, melanins either chemically synthesized or extracted from animals, which presents significant challenges for large-scale production. Bacteria have been used as biocatalysts to synthesize biomaterials due their fast growth amenability genetic engineering...
Erythropoietin (EPO)-induced cellular signaling through the EPO receptor (EPOR) is a fundamental pathway for modulation of behavior and activity. In our previous work, we showed in primary human astrocytes that multivalent display on surface semiconductor quantum dots (QDs) mediates augmented JAK/STAT signaling, concomitant 1.8-fold increase expression aquaporin-4 (AQPN-4) channel proteins, 2-fold AQPN-4-mediated water transport Our hypothesis this enhanced involves simultaneous ligation...
DNA nanostructures have proven potential in biomedicine. However, their intracellular interactions─especially cytosolic stability─remain mostly unknown and attempts to discern this are confounded by the complexities of endocytic uptake entrapment. Here, we bypass evaluate structural stability directly live cells. Commonly used structures─crosshairs a tetrahedron─were labeled with multistep Förster resonance energy transfer dye cascade microinjected into cytosol transformed primary Energy...
Gold nanoparticles (AuNPs) attached to the extracellular leaflet of plasma membrane neurons can enable generation action potentials (APs) in response brief pulses light. Recently described techniques stably bind AuNP bioconjugates directly proteins (ion channels) robust AP mediated by photoexcited conjugate. However, a strategy that binds non protein-specific manner could represent simple, single-step means establishing light-responsiveness multiple types excitable contained same tissue. On...
Traumatic injury is often associated with hemorrhagic shock. Liposome-encapsulated hemoglobin (LEH) being developed as an artificial oxygen carrier to address post-hemorrhage and volume deficit. Here, we report a new composition of LEH based on the use polyethylene glycol (PEG2K ) conjugated nonphospholipid hexadecylcarbamoylmethylhexadecanoate (HDAS) modify surface particles. was manufactured by high-pressure homogenization method using dipalmitoylphosphatidylcholine (∼38 mol%), cholesterol...
Two types of cationic polyfluorene copolymers (FHQ, FPQ) with a same π-conjugated structure but different counterions (bromide (BR), tetraphenylborate (PB)) were synthesized and studied as fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). The accompanying the polymer chain for charge compensation are expected perturb complexation modify fine-structure D/A complex on molecular scale, which may influence competition between desirable FRET energy-wasting...
A main goal of bionanotechnology and nanoparticle (NP)-mediated drug delivery (NMDD) continues to be the development novel biomaterials that can controllably modulate activity NP-associated therapeutic cargo. One desired subcellular locations for targeted in NMDD is plasma membrane. However, controlled hydrophobic cargos membrane bilayer poses significant challenges including cargo precipitation lack specificity. Here, we employ a liquid crystal NP (LCNP)-based system partitioning model dye...
We have previously reported the flavonoid, quercetin, as a metabolic regulator and inhibitor of myofibroblast differentiation in vitro. Our current study evaluated effects topical application quercetin on corneal scar development using two different animal models followed by RNA analysis Wild-type C57BL/6J mice were anesthetized epithelium stroma manually debrided, (0.5, 1, 5, or 50 mM) vehicle application. Corneal scarring was assessed for 3 weeks slit lamp imaging clinically scored. In...
Cationic poly[9,9′-bis[6′′-(N,N,N-trimethylammonium)hexyl]fluorene-co-alt-phenylene]s with five different counterions (CIs) were synthesized and studied as fluorescence resonance energy transfer (FRET) donors (D) to dye-labeled DNA (FRET acceptor, A). The polymers CIs show the same π-conjugated electronic structure similar absorption (λabs = ∼380 nm) photoluminescence (λPL ∼420 emission spectra in water. accompanying polymer chain are expected affect D/A complexation modify D−A...
The solvent effects were studied in fluorescence resonance energy transfer (FRET) from a cationic polyfluorene copolymer (FHQ, FPQ) to fluorescein (Fl)-labelled oligonucleotide (ssDNA-Fl). Upon addition of dimethyl sulfoxide (DMSO), the optical properties polymers and probe dye substantially modified FRET-induced PL signal was enhanced 3.8–37 times, relative that phosphate buffer solution (PBS). hydrophobic interaction between ssDNA-Fl is expected decrease presence DMSO, which induces weaker...
Current challenges in photodynamic therapy (PDT) include both the targeted delivery of photosensitizer (PS) to desired cellular location and maintenance PS efficacy. Zinc phthalocyanine (ZnPc), a macrocyclic porphyrin potent for PDT, undergoes photoexcitation generate reactive singlet oxygen that kills cells efficiently, particularly when delivered plasma membrane. Like other commonly employed PS, ZnPc is highly hydrophobic prone self-aggregation aqueous biological media. Further, it lacks...
A polymer-surfactant micellar complex has been studied as a fluorescence resonance energy transfer (FRET) donor to fluorescein-labeled DNA (ssDNA-Fl). In water, the molar absorptivity and quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) are substantially increased in presence non-ionic surfactants. TEM microscopic study shows formation nanowire c-PFP About 400% enhancement FRET signal is measured c-PFP/ssDNA-Fl with Brij 30, relative that without The amplification...
Two-photon absorption properties of 1,4-bis{4′-[N,N-bis(6′′-trimethylammoniumhexyl)amino]styryl}benzene tetrabromide (C1) and its inclusion complexes (ICs) with cyclodextrins (CDs) have been studied. Upon complexation CDs, the spectra C1 showed a slight red shift, whereas emission blue shift concomitant increase in fluorescence quantum efficiency. A Stern−Volmer study using K3Fe(CN)6 as quencher revealed significant reduction photoinduced charge transfer quenching, accord IC formation....