A5062585810- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Extracellular vesicles in disease
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Renal and related cancers
- Comparative Animal Anatomy Studies
- Endometriosis Research and Treatment
- Kruppel-like factors research
- T-cell and Retrovirus Studies
- Congenital heart defects research
- Physiological and biochemical adaptations
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Periodontal Regeneration and Treatments
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Inflammatory mediators and NSAID effects
- Polyamine Metabolism and Applications
- Digestive system and related health
- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
University of Concepción
2018-2025
Universidade de São Paulo
2011-2015
Universidade Brasil
2012
As observed in other species during pregnancy, bovine placentomes are assumed to suppress classical MHC I antigens, whereas overexpress non-classical be recognized but not rejected by maternal immune system. However, cloned antigens were early the pregnancy instead of ones. Then, investigated immunohistochemistry (N=23) and RT-qPCR (N=16) control gestation near term. In controls, IL-A88 staining was present all stages, majorly tissues, Qa-2 reacted only Clones had an opposite pattern....
Major histocompatibility complex class I (MHC-I) gene expression in the placenta is modulated to tailor maternal immune response fetal antigens during pregnancy. This study evaluated MHC-I through immunohistochemistry (IHC) using an anti-mouse preimplantation embryo development (PED) clone Qa-2 and anti-bovine leukocyte antigen (BoLA) monoclonal antibody IL-A88 (n = 23), as well RT-qPCR 17) for classical non-classical (BoLA-NC) genes control cloned bovine placentomes early near-term...
During early development, embryos secrete extracellular vesicles (EVs) that participate in embryo–maternal communication. Among other molecules, EVs carry microRNAs (miRNAs) interfere with gene expression target cells; miRNAs Embryo selection based on secreted may have an impact bovine breeding programs. This research aimed to evaluate the size, concentration, and miRNA content of by different developmental potential, during compaction period (days 3.5–5). Individual culture media from...
Xenotransplantation of spermatogonial stem cells (SSCs) has become a popular topic in various research fields because manipulating these can provide insights into the mechanisms associated with germ cell lines and entire spermatogenesis process; moreover, be used several biotechnology applications. To achieve successful xenotransplantation, vitro microenvironment which SSCs are cultured should an ideal for self-renewal similar to vivo testicular microenvironment. The age donor, correct...
The modulation of inflammation is pivotal for uterine homeostasis. Here we evaluated the effect oestrus cycle on expression pro-inflammatory and anti-inflammatory markers in a cellular model induced fibrosis. Mare endometrial stromal cells isolated from follicular or mid-luteal phase were primed with 10 ng/mL TGFβ alone combination either IL1β, IL6, TNFα (10 each) all together 24 h. Control not primed. Messenger miRNA analyzed using real-time quantitative PCR (RT-qPCR). Cells cytokines...
In mesenchymal stem cells (MSCs), it has been reported that prostaglandin E2 (PGE2) stimulation of EP2 and EP4 receptors triggers processes such as migration, self-renewal, survival, proliferation, their activation is involved in homing. The aim this work was to establish a genetically modified adipose (aMSC) model which receptor genes were edited separately using the CRISPR/Cas9 system. After edition, evaluated if expression MSC surface markers affected, well migration capacity vitro...
O Procyon cancrivorus é um carnívoro silvestre amplamente distribuído e relativamente comum, mas ainda pouco estudado, existindo poucos trabalhos relatando sobre a biologia dessa espécie. Este trabalho tem como objetivo, caracterizar morfologicamente glândula pineal de cancrivorus, através estudos macro, microscópicos, radiográficos compará-los com outros animais já estudados. Foram utilizados quatro adultos ambos os sexos, provenientes do Criatório Científico CECRIMPAS, IBAMA (Processo nº...
Equine endometrial and adipose mesenchymal stem cells (eMSCs aMSCs, respectively) were isolated from the same donors of thoroughbred mares. The displayed characteristic features MSCs, including trilineage mesodermal also neurogenic differentiation. We evaluated influence cellular origin on their transcriptome profile. Cellular RNA was sequenced extracellular vesicles (EVs) obtained conditioned medium cultured in depleted EVs, microRNA (miRNA) cargo analyzed by sequencing. Differential...
The potential applications of neural stem cells (NSC) in the therapy degenerative and traumatic diseases central nervous system (CNS) have aroused great scientific interest. NSCs can be recovered from specific areas CNS fetuses, embryos adult brain as well, under appropriate culture conditions, may induced to differentiate into three major cell types, neurons, astrocytes oligodendrocytes. main neurogenic mammals are subventricular zone (SVZ) dentate gyrus (DG) hippocampus, yet other been...
Mare endometrosis is a major reproductive problem associated with low fertility and characterized by persistent inflammation, TGFβ-1 signaling, consequently, extracellular matrix deposition, which compromises endometrial glands. Mesenchymal stem cell-based products (MSCs), such as vesicles (EVs), have gained attention due to the regulatory effects exerted their miRNA cargo. Here, we evaluated impact of preconditioning equine adipose mesenchymal cells for short or long periods on...
Este estudo teve como objetivo analisar atividade proliferativa das células trofoblásticas, através da quantificação de AgNORs, em diferentes regiões placenta bovina ao longo gestação. Foram utilizados 28 úteros, sendo estes agrupados acordo com as idades gestacionais: grupo I (60-120 dias); II (121- 170 III (171-220 dias) e IV (221-290 dias). Foi encontrado um número significativamente maior AgNORs nas trofoblásticas gigantes (CTG) relação às mononucleadas (CTM) (p<0,001) todas grupos...
As Regiões Organizadoras de Nucléolo (NORs - nucleolar organizer regions) são utilizadas para descrever regiões cromatina coradas por Nitrato Prata e estão relacionados com a atividade síntese RNAr agilidade rapidez na proliferação celular nos tecidos estudados. O objetivo deste trabalho foi relacionar quantidade AgNORs, proliferativa o estágio da gestação em equinos, utilizando coloração Prata. Os anexos embrionários foram coletados, fixados solução formaldeído tamponado 10%, emblocadas...
Prostaglandin E2 (PGE2) is an important mediator of cellular responses, playing a key role in limiting inflammation. PGE2 acts distinctly through one four EP receptors. In MSCs, it stimulation EP2 and EP4 receptors triggers processes such as migration, self-renewal, survival, proliferation, their activation involved homing. has been proposed factor MSC immunomodulation. The CRISPR-Cas9 system adapted successfully to edit the genome animals. Loss equine adipose mesenchymal stem cells...
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)
Abordagens proteomicas revolucionaram a analise de amostras biologicas por permitir identificacao padroes ainda nao caracterizados expressao proteinas e/ou suas interacoes e modificacoes pos-traducionais em diferentes tecidos, como placenta ou embrioes fetos estagios desenvolvimento. Esta nova fronteira conhecimento se constitui atualmente numa ferramenta fundamental para elucidacao problemas biologicos, fornecer informacoes que podem ser obtidas metodos mais focalizados, com alvos...