A5040649218- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Renal and related cancers
- Congenital heart defects research
- Comparative Animal Anatomy Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Congenital Anomalies and Fetal Surgery
- Balance, Gait, and Falls Prevention
- Nutrition and Health in Aging
- Epigenetics and DNA Methylation
- Vitamin D Research Studies
- Infant Nutrition and Health
- Neurogenesis and neuroplasticity mechanisms
- Antioxidant Activity and Oxidative Stress
- Extracellular vesicles in disease
- Body Composition Measurement Techniques
- Organ and Tissue Transplantation Research
- Wound Healing and Treatments
- Meat and Animal Product Quality
- Xenotransplantation and immune response
Universidade Federal de São Carlos
2023-2025
Universidade de São Paulo
2014-2024
University of Guelph
2021-2023
Universidade Federal do Rio Grande do Norte
2017
Leiden University Medical Center
2016
Associação Brasileira de Oncologia Veterinária
2014
Universidade Brasil
2012
Universidade Católica de Santos
2010
Abstract: Amnion-derived mesenchymal stem cells (AMSCs) are multipotent with an enhanced ability to differentiate into multiple lineages. AMSCs can be acquired through noninvasive methods, and therefore exempt from the typical ethical issues surrounding cell use. The objective of this study was isolate characterize a cat amniotic membrane for future application in regenerative medicine. were harvested after mechanical enzymatic digestion amnion. In culture medium, adhered plastic dish...
Abstract Aims Cross‐sectional studies demonstrate an association between low serum levels of vitamin D and slower gait speed in older individuals. However, longitudinal remain inconclusive. This study investigates whether deficiency insufficiency are risk factors for the incidence slowness. Materials Methods A total 2815 participants from English Longitudinal Study Ageing (ELSA), aged ≥60 years with a baseline >0.8 m/s, were followed six years. Baseline [25(OH)D] categorized as...
Potential mechanisms involved in neural differentiation of adipocyte derived stem cells (ADSCs) are still unclear. In the present study, extracellular vesicles (EVs) were tested as a potential mechanism neuronal cells. order to address this, ADSCs and neurons (BRC) established primary culture co-culture at three timepoints. Furthermore, we evaluated protein transcript levels differentiated from same timepoints, confirm phenotype change linage. Importantly, neuron-derived EVs cargo originated...
ABSTRACT Human germ cells originate in an extragonadal location and have to migrate colonize the gonadal primordia at around seven weeks of gestation (W7, or five post conception). Many are lost along way should enter apoptosis, but some escape can give rise cell tumors. Due common somatic origin gonads adrenal cortex, we investigated whether ectopic were present human adrenals. Germ expressing DDX4 and/or POU5F1 male female adrenals first second trimester. However, contrast what has been...
The generation of mature gametes and competent embryos in vitro from pluripotent stem cells has been successfully achieved a few species, mainly mice, with recent advances humans scarce preliminary reports other domestic species. These biotechnologies are very attractive as they facilitate the understanding developmental mechanisms stages that generally inaccessible during early embryogenesis, thus enabling advanced reproductive technologies contributing to animals high genetic merit short...
PURPOSE: To describe a new technique for isolation of mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits. METHODS: Olfactory (OSCs) were retrieved under cribriform plate Ethmoid bone. Several assays accomplished to characterize cell and attest its viability vitro. The submitted flow cytometry with antibodies CD34, CD45, CD73, CD79, CD90 CD105 also they induced differentiate three lineages. Functional evaluation involved analysis vitro growth behavior, colony...
Pluripotent stem cells are believed to have greater clinical potential than mesenchymal due their ability differentiate into almost any cell type of an organism, and since 2006, the generation patient-specific induced pluripotent (iPSCs) has become possible in multiple species. We hypothesize that different types respond differently reprogramming process; thus, goals this study were isolate characterize equine adult fetal induce these pluripotency for future regenerative translational...
Primordial germ cells (PGCs) are precursors of gametes that can generate new individuals throughout life in both males and females. Additionally, PGCs have been shown to differentiate into embryonic (EGCs) after vitro culture. Most studies investigating germinative performed rodents humans but not dogs (Canis lupus familiaris). Here, we elucidated the dynamics expression pluripotent (POU5F1 NANOG), germline (DDX4, DAZL DPPA3), epigenetic (5mC, 5hmC, H3K27me3 H3K9me2) markers important for...
In this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. total, three pEFs lines reprogrammed, cultured at least 15 passages, characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous endogenous genes, immunofluorescence). Two piPSC further...
Xenotransplantation of spermatogonial stem cells (SSCs) has become a popular topic in various research fields because manipulating these can provide insights into the mechanisms associated with germ cell lines and entire spermatogenesis process; moreover, be used several biotechnology applications. To achieve successful xenotransplantation, vitro microenvironment which SSCs are cultured should an ideal for self-renewal similar to vivo testicular microenvironment. The age donor, correct...
The event of cellular reprogramming into pluripotency is influenced by several factors, such as in vitro culture conditions (e.g., medium and oxygen concentration). Herein, bovine iPSCs (biPSCs) were generated different levels tension (5% or 20% oxygen) supplementation (bFGF bFGF + LIF 2i—bFL2i) to evaluate the efficiency induction maintenance vitro. Initial was observed all groups bFL2i initially resulted a superior number colonies. However, low led loss self-renewal maintenance. All clonal...
To analyse whether dynapenic abdominal obesity is a risk factor for Metabolic syndrome (MetS) and its components in individuals 50 years of age or older.
Abstract Aim Although diabetes is a risk factor for walking speed decline in older adults, it remains unclear how glycaemic control [assessed by glycated haemoglobin (HbA1c)] might affect the long‐term trajectories of speed. We investigated whether status accelerates and this differs depending on previous mobility conditions. Materials Methods In total, 3202 individuals aged ≥60 years from English Longitudinal Study Ageing (ELSA) were classified at baseline after 4 8 follow‐up according to...
O quati (Nasua nasua) é um animal que pertence à Família Procyonidae. Foram utilizados três animais ortotanasiados, de ambos os sexos, provenientes do Criatório Científico Animais Silvestres, Centro Universitário Fundação Ensino Octávio Bastos (Cecrimpas, Unifeob) autorizado pelo IBAMA (Proc.02027.003731/04-76). Para a análise macroscópica, as línguas foram retiradas, analisadas e foto-documentadas. microscópica, processadas rotineiramente pela técnica microscopia eletrônica varredura...
Sapajus apella is a wild monkey of South America distributed across almost all Brazil. This species adapts to domesticated life and reproduces easily. The present study describes the macro- microscopic morphology male genital organs (penis, penis bone, glans penis, prepuce, bulb urethra) apella. Four monkeys were used in this study. For macroscopic description, genitals dissected, examined photographed. analysis, samples stained by HE Tricom Masson analyzed scanning electron microscopy light...
Purpose: Amniotic membrane stem cells have a high capacity of proliferation, cell expansion, and plasticity, as well immunomodulatory properties that contribute to maternal-fetal tolerance. Owing the lack research on human amniotic at different gestational stages, canine model is considered ideal because its genetic physiological similarities. We aimed characterize (CAM) lineage in stages evaluate expression genes. Materials Methods: Twenty CAMs from early (20– 30 days) (n=7), mid- (31– 45...
Several opportunities for embryo development, stem cell maintenance, fate, and differentiation have emerged using induced pluripotent cells (iPSCs). However, the difficulty in comparing bovine iPSCs (biPSCs) with embryonic (ESCs) was a challenge many years. Here, we reprogrammed fetal fibroblasts by transient expression of four transcription factors (Oct4, Sox2, Klf4, c-Myc, collectively termed "OSKM" factors) cultured iPSC medium, supplemented bFGF, bFGF2i, leukemia inhibitory factor (LIF),...
Contents The aim of this study was to further clarify the mechanisms involved in inducing pluripotency using canine foetal fibroblast cells. two pluripotency‐related transcription factors, OCT 4 and SOX 2, coupled a fluorescent reporter gene were transduced, individually or combination, lentiviral system. Stable transgenic cell lineages obtained cells showed be highly responsive integration expression human 2 4, also depending on amount virus used for incubation. Such positive results are...
Turner syndrome (TS) is a genetic disorder in females with X Chromosome monosomy associated highly variable clinical features, including premature primary gonadal failure leading to ovarian dysfunction and infertility. The mechanism of development primordial germ cells (PGCs) their connection TS poorly understood. An vitro model PGCs from would be beneficial for investigating epigenetic factors that influence cell specification. Here we investigated the potential reprogramming peripheral...