A5087237861- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Electrospun Nanofibers in Biomedical Applications
- Neurogenesis and neuroplasticity mechanisms
- Sperm and Testicular Function
- Comparative Animal Anatomy Studies
- Nerve injury and regeneration
- Congenital Anomalies and Fetal Surgery
- Livestock and Poultry Management
- Environmental Toxicology and Ecotoxicology
- Muscle Physiology and Disorders
- Cancer Research and Treatments
- Pluripotent Stem Cells Research
- Bone and Joint Diseases
- Physiological and biochemical adaptations
- Rabbits: Nutrition, Reproduction, Health
- Animal Nutrition and Physiology
- Spinal Cord Injury Research
- Hereditary Neurological Disorders
- Spinal Dysraphism and Malformations
- Neurobiology and Insect Physiology Research
- Chronic Kidney Disease and Diabetes
- Congenital heart defects research
Universidade Estadual do Maranhão
2006-2022
Universidade Federal do Piauí
2014-2019
Universidade de São Paulo
2009-2017
Hôpital de la Conception
2017
Universidade Brasil
2009
PURPOSE: Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from marrow and human immature dental-pulp (hIDPSC) in model induced osteonecrosis femoral head (ONFH). METHODS: Eight sheep were divided three experimental groups. First group was composed by four animals with ONFH ethanol through central decompression (CD), for control without any treatment. The second third compose two animals, six weeks after induction received heterologous MSC (CD...
PURPOSE: To evaluate different protocols to isolate stem cells from ovine umbilical cord blood and adipose tissue. METHODS: There were used 5 samples of perirenal tissue 10 female sheep. All the obtained through surgery, harvest aseptic samples. 3 for obtainment culture 4 cells. RESULTS: It was possible observe only one successful protocol When analyzing techniques obtain cells, methods effective as well. Through colony forming unit assay, there 58 colonies after seven days in culture. Flow...
Duchenne muscular dystrophy (DMD) is a human disease characterized by progressive and irreversible skeletal muscle degeneration caused mutations in genes coding for important proteins. Unfortunately, there no efficient treatment this disease; it causes loss of motor ability until death. The canine model (golden retriever dystrophy) similar to DMD, showing clinical signs. Fifteen dogs were followed from birth closely observed Dogs had their status confirmed polymerase chain reaction analysis...
Background Tissue engineering has been shown to exhibit great potential for the creation of biomaterials capable developing into functional tissues. Cellular expansion and integration depends on quality surface-determinant factors scaffold, which are required successful biological implants. The objective this research was characterize evaluate in vitro characteristics rabbit bone marrow mesenchymal stem cells (BM-MSCs) associated with a bacterial cellulose membrane (BCM). We assessed...
O ensino médico veterinário é regido pelas diretrizes curriculares nacionais (Brasil, 2019), através destas, as universidades estabelecem seus projetos pedagógicos de curso (PPC). Esta pesquisa envolveu busca ativa documentos (PPC e ementas) com intuito visualizar a oferta Ciência em Animais Laboratório (CAL) Diagnóstico por Imagem (DI) no Medicina Veterinária da Universidade Estadual do Maranhão, priorizando os Projetos Político Pedagógicos período 1995 2021. Os dados obtidos evidenciaram...
Purpose: To investigate the therapeutic potential of human immature dental pulp stem cells in treatment chronic spinal cord injury dogs. Methods: Three dogs different breeds with SCI were presented as animal clinical cases. Human injected at three points into cord, and animals evaluated by limb function magnetic resonance imaging (MRI) pre post-operative. Results: There was significant improvement from Olby Scale, though it not supported data provided MRI sign evaluation. Conclusion: cell...
PURPOSE: To describe a new technique for isolation of mesenchymal stem cells (MSCs) population from the olfactory mucosa in rabbits. METHODS: Olfactory (OSCs) were retrieved under cribriform plate Ethmoid bone. Several assays accomplished to characterize cell and attest its viability vitro. The submitted flow cytometry with antibodies CD34, CD45, CD73, CD79, CD90 CD105 also they induced differentiate three lineages. Functional evaluation involved analysis vitro growth behavior, colony...
Mesenchymal stem cells have been widely used in the treatment of various chronic diseases. The objective this survey was to evaluate therapeutic and regenerative potential from adipose tissue (ASCs) milk production recovery repair injury mastitis goats treated with antimicrobial agents prior cell therapy. After diagnosis gentamicin, eight lactating were selected for cellular subsequent therapy, physical-chemical analysis milk, ultrasonographic histopathological examinations. ASCs taken...
Background: There are few studies on stem cell isolation in wild animals that provide and culture protocols of these cells vitro. Among the species studied, we present collared peccary (Tayassu tajacu) as a model with potential to obtain use MSC preclinical studies. These phylogenetically close domestic pig, popularly known peccaries found naturally South America, Central America United States. The aim study was establish protocol for isolation, vitro expansion, differentiation assessment...
O objetivo do trabalho foi avaliar a ação acaricida extrato de nim sobre forma pomada, no tratamento da acaríase em camundongos (Mus musculus var. albina L.) naturalmente infestados por Myobia musculi Schrank e Myocoptes musculinus Koch. Foram selecionados 20 animais parasitados distribuídos quatro grupos: controle (vaselina), 5%, 10% 20%. Os foram submetidos ao cada 48h mantidos confinados gaiolas, com alimento água ad libitum acompanhados diariamente longo 24 dias. Houve redução...
PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten of both genders were used the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone aspirates where taken tibial ridge and seeded on culture plates for isolation, expansion Flow Cytometry (expression markers Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor showed colonies characterized by presence...
Resumo: As biotécnicas da reprodução são importantes ferramentas para conservação de espécies domésticas e silvestres, pois permitem a recuperação uso futuro material reprodutivo. O objetivo deste estudo foi avaliar parâmetros morfológicos CCOs cutias, obtidos pela técnica fatiamento do ovário utilização em protocolos maturação fecundação na produção in vitro embriões. Foram utilizadas dezessete cutias fêmeas NEPAS, CCA- UFPI, com idade peso médios 3,9 anos 2,16Kg, respectivamente, que foram...
A distrofia muscular de Duchenne (DMD) é uma doença origem genética, cuja principal manifestação clínica enfraquecimento e atrofia progressiva dos músculos. Os cães da raça Golden Retriever podem apresentar muscular, com características genotípicas fenotípicas muito próximas à humana, sendo considerado o modelo animal mais apropriado para estudo DMD. Foram realizadas radiografias torácicas látero-laterais dorsoventrais 10 afetados pela objetivo relatar as alterações radiográficas associadas...
PURPOSE: The use of the collared peccary as an experimental model for ischemic nephropathy. METHODS: A total 12 (Tayassu tajacu) was used and nephropathy induced in six these animals that constituted group (G1) while other formed control (G2). Ischemic surgically by partial occlusion left renal artery. disease course assessed hematological tests, serum chemistry, urinalysis, ultrasound (US) doppler function artery before induction, at five, 10, 15 20 days after surgery. Twenty occlusion,...
ABSTRACT There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their vitro behavior. Regenerative medicine science undergoing improvement which develops treatments as cell therapy using somatic cells. In several studies, adipose tissue presented source multipotent adult cells that has advantages over other sources. This aimed to characterize and evaluate tagging from agoutis (Dasyprocta prymonolopha), with fluorescent...
This present work describe the peripheral blood cell morphology from Chelonoids carbonaria.To do this were used ten animal specimens clinically healthy, six female and four male, submitted to collect by jugular vein.Blood was collected prepare smears, without use of anticoagulants.The slides stained analyzed microscopically morphology.The mature erythrocytes had an ellipsoid shape a grain-free nucleus; immature ones circular.The leukocytes, granulocytes agranulocytes also heterophils...
This present work describe the peripheral blood cell morphology from Chelonoids carbonaria.To do this were used ten animal specimens clinically healthy, six female and four male, submitted to collect by jugular vein.Blood was collected prepare smears, without use of anticoagulants.The slides stained analyzed microscopically morphology.The mature erythrocytes had an ellipsoid shape a grain-free nucleus; immature ones circular.The leukocytes, granulocytes agranulocytes also heterophils...
The aim of this estudy was to establish the levels serum total protein, albumin, globulin, alanine aminotransferase (ALT), aspartate (AST), calcium, phosphorus, urea, creatinine, bilirubin and glucose during pregnancy in agoutis.Animals: Twelve pregnant agouti from Center for Study Preservation Wild Animals (CSPWA) Federal University Piauí (UFPI) were used research.After identification estrus, day coverage confirmed by means vaginal cytology with visualization spermatozoa (day zero)...