A5031862197- Nitric Oxide and Endothelin Effects
- Immune Response and Inflammation
- Dialysis and Renal Disease Management
- Anesthesia and Pain Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Chemokine receptors and signaling
- Cancer-related molecular mechanisms research
- Atrial Fibrillation Management and Outcomes
- Autophagy in Disease and Therapy
- Central Venous Catheters and Hemodialysis
- MicroRNA in disease regulation
- Anesthesia and Neurotoxicity Research
- Platelet Disorders and Treatments
- Inflammatory mediators and NSAID effects
- Asthma and respiratory diseases
- Cytomegalovirus and herpesvirus research
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac and Coronary Surgery Techniques
- Retinal Diseases and Treatments
- Reproductive System and Pregnancy
- Magnolia and Illicium research
- Blood Coagulation and Thrombosis Mechanisms
- Nausea and vomiting management
- Transplantation: Methods and Outcomes
- Neuropeptides and Animal Physiology
China Medical University Hospital
2016-2025
China Medical University
2016-2025
National Yang Ming Chiao Tung University
1998-2024
Beijing Anzhen Hospital
2021-2024
Capital Medical University
2021-2024
China Medical University
2011-2023
National Institute for Parasitic Diseases
2022
Feng Chia University
2014-2020
Taipei Medical University Hospital
2012-2018
Pain and Rehabilitation Medicine
1991-2014
The esterases of human leukocytes were studied both by cytochemical methods and polyacrylamide gel electrophoresis. aim the study was to clarify specificity various establish specific for demonstrating types in blood cells. Nine bands isoenzymes separable electrophoresis at pH 4.0. Bands 1, 2, 7, 8 9 best demonstrated naphthol AS-D chloroacetate represented activity esterase as granulocytes. 3, 4, 5 6 naphthyl esters "nonspecific esterases" monocytes, platelets plasma "Aminocaproate...
Abstract —The activity and protein expression of endothelial nitric oxide synthase (eNOS) inducible NOS (iNOS) were investigated during the development hypertension in spontaneously hypertensive rats (SHR). SHR Wistar-Kyoto (WKY) studied at three different ages: 4, 14 to 17, 63 weeks age. After treatment with saline or lipopolysaccharide (LPS, 10 mg/kg IV) for 3 hours, aortas removed measurement assay by [ H]- l -citrulline formation method Western blot analysis, respectively. Plasma levels...
We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with mixture azide hydrogen peroxide, which is then followed by an indirect procedure. Comparison studies showed no significant loss antigenicity or morphological details this pre-treatment. The most useful evaluating cell-specific antigens on specimens that have abundant activity, such as blood bone marrow.
Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid lactic acid, which lower pH thereby inhibit growth of pathogens or allow probiotic bacteria to compete with for epithelial adhesion sites nutrients. The transmural migration enteric organisms into peritoneal cavity cause peritonitis in dialysis (PD) patients. We hypothesized that composition intestinal microbiota regard species differed between PD patients healthy controls. aim study was investigate...
Abstract Background Dementia, which leads to disability, is one of the important diseases occurring among older populations. However, exact mechanism disease remains unknown. The potential risk factor general anesthesia (GA) in development dementia a controversial topic. Therefore, this study aimed evaluate association between previous exposure different GA types and incidence dementia. Methods Using claims data 1 million insured residents covered by Taiwan's universal health insurance from...
In Brief We tested baicalin for its antiinflammatory and analgesic effects (and the mechanisms) in a rat model of carrageenan-evoked thermal hyperalgesia. Pre- or posttreatment with (10, 30, 100 mg/kg intraperitoneally) caused significant effect similar dose-matched ibuprofen. Furthermore, dose-dependently attenuated tumor necrosis factor-α (from 3510 ± 150 pg/mL to 2860 148 1480 210 pg/mL), interleukin (IL)-1β 3210 2200 140 750 95 IL-6 58.5 9.8 38.5 9.0 21.0 8.1 ng/mL) formation but...
Abstract RANTES (Regulated on activation, normal T-cell expressed and secreted), recruits circulating leukocytes augments inflammatory responses in many clinical conditions. Inflammatory ischemia-reperfusion injury (IRI) significantly affect the unfavorable outcomes of acute kidney (AKI) that infiltrating immune cells are important mediators AKI. However, significance AKI whether hypoxia-induced LncRNAs involved regulatory process not known. Here we show that, IRI mice model, significant...
Toll-like receptor 4 (TLR4) plays a major role mediating endotoxin-induced cellular inflammation and regulates vascular smooth muscle cell (VSMC) proliferation, which is related to atherogenesis restenosis. This study was conducted investigate the mechanisms involved in lipopolysaccharide (LPS)-induced TLR4 expression VSMCs.Stimulation of human aortic cells (HASMCs) with LPS significantly increased expression. The increase regulated by nicotinamide adenine dinucleotide phosphate (NADPH)...
Cardiopulmonary bypass (CPB) induces inflammatory responses, and effective endogenous homeostasis is important for preventing systemic inflammation. We assessed whether plasma exosomal microRNAs in patients undergoing cardiac surgery with CPB are involved the regulation of responses. Plasma samples were isolated from (n = 21) at 5 specified time points: pre-surgery, pre-CPB 2 hours (h), 4 h 24 after began. TNF-α expression was increased began compared to that pre-surgery samples. IL-8 IL-6...
Parkinson's disease (PD) is the second most common age-related neurodegenerative disorder with limited clinical treatments. The occurrence of PD includes both genetic and environmental toxins, such as pesticides paraquat (PQ), major contributors to pathology in invertebrate mammalian models. Calycosin, an isoflavone phytoestrogen, has multiple pharmacological properties, including neuroprotective activity. However, paucity information regarding potential calycosin on PQ-induced...
Oral squamous cell carcinomas (OSCCs), like several solid tumours, contain heterogeneous subpopulations of a small subset cancer cells, termed stem cells (CSCs), that are highly relevant to metastasis and invasive properties. CSCs have also shown high capacity survive against various stressful environments, such as hypoxia. However, the molecular underpinnings behind potential under this stress remain unclear. The current study aimed investigate significance autophagy systems in oral CSC...
In Brief The postoperative period is associated with an increased production of cytokines, which augment pain sensitivity. We investigated the hypothesis that epidural clonidine premedication and patient-controlled analgesia (PCEA) including would decrease release proinflammatory (interleukin (IL)-6, IL-1β, IL-8, tumor necrosis factor (TNF)-α) antiinflammatory (IL-1 receptor antagonist (RA)) cytokines in patients who underwent elective colorectal surgery they provide better analgesia. Forty...
Lipopolysaccharide (LPS) interacts with toll-like receptor 4 (TLR4) and induces proliferation of vascular smooth muscle cells (VSMCs) which plays a causal role in atherogenesis. The TLR4 expression regulation LPS-stimulated VSMCs remains unclear. mRNAs often contain AU-rich elements (AREs) their 3' untranslated regions (3'UTR) have high affinity for RNA-binding proteins. It is not know whether the protein, human antigen R (HuR), regulates aortic (HASMCs). Stimulation HASMCs LPS significantly...
BACKGROUND Distal radius fracture is recognized as an osteoporosis-related in aged population. If another occurs a short period, it represents prolonged hospitalization and considerable economic burden to the society. We evaluated relationship between distal subsequent hip within 1 year, especially critical time age. METHODS identified newly diagnosed patients 2000 2006 exposed cohort (N = 9,986). A comparison 81,227) was randomly selected from without same year of cohort. The subjects were...
ABSTRACT Chlorhexidine has been widely used for infection control. Although the use of chlorhexidine-impregnated catheters reduced catheter-related infections, chlorhexidine-resistant Staphylococcus aureus emerged. The correlation between existence genes qacA and qacB ( qacA/B ) in methicillin-resistant (MRSA) isolates effectiveness prevention MRSA infections is unknown. Sixty methicillin-sensitive (MSSA) 96 from blood cultures different patients were collected, a case-control study was...
SUMMARY Newborn larvae (NBL) and adult (Ad) stage-specifically expressed genes or members of gene families Trichinella spiralis were identified by suppression subtractive hybridization (SSH)†. Six cDNA clones as NBL stage-specific, including 1 member the T. family encoding glutamic acid-rich proteins, 2 novel serine proteases, closely related proteins that are a deoxyribonuclease II (DNase II)-like clone with no similarity to known genes. Four stage-specific homologues retinoid X receptor,...
Abstract We used ion-exchange column chromatography and electrophoresis on polyacrylamide gel to compare the acid phosphatase isoenzymes of prostate leukocytes. The major isoenzyme is band 2A; only a trace 2B was observed. However, leukocytes are 4 2B, small amount 2A three isolated from or gland react antiserum prepared against aicd seminal fluid. Acid phosphatases other than mentioned above did not interact with antiserum.
Platelet activation and its interaction with leukocytes play an important role in atherothrombosis. Cardiovascular diseases resulted from atherothrombosis remain the major causes of death worldwide. Gallic acid, a constituent red wine tea, has been believed to have properties cardiovascular protection, which is likely be related antioxidant effects. Nonetheless, there were few inconsistent data regarding effects gallic acid on platelet function. Therefore, we designed this vitro study...