A5068612571- DNA Repair Mechanisms
- Vanadium and Halogenation Chemistry
- Effects of Radiation Exposure
- Cancer-related Molecular Pathways
- Advanced Nanomaterials in Catalysis
- Polyoxometalates: Synthesis and Applications
- Cell death mechanisms and regulation
- Reproductive Biology and Fertility
- Carcinogens and Genotoxicity Assessment
- Animal Genetics and Reproduction
- Melanoma and MAPK Pathways
- Congenital Heart Disease Studies
- Genetic factors in colorectal cancer
- Cancer, Hypoxia, and Metabolism
- Radiation Therapy and Dosimetry
- Cardiac Valve Diseases and Treatments
- T-cell and Retrovirus Studies
- Aortic Disease and Treatment Approaches
- Thermal Regulation in Medicine
- Pluripotent Stem Cells Research
- Heat shock proteins research
- Cardiac Arrest and Resuscitation
- bioluminescence and chemiluminescence research
- Radiation Dose and Imaging
- NF-κB Signaling Pathways
Tohoku University
2014-2024
The University of Tokyo
2002-2023
Hiroshima University
2011-2017
Hamamatsu University
2017
Showa Pharmaceutical University
2012
Fujifilm (Japan)
2009-2011
Tokyo University of Science
2004-2010
Niigata University
2009-2010
Jichi Medical University
2010
Niigata University of Health and Welfare
2009
Tumor tissues and adjacent normal in 12 colorectal cancers were examined for quantitative differences in: i) activity of DNA-dependent protein kinase (DNA-PK), which functions DNA double-strand breads repair, ii) mRNA levels Ku70, Ku80, DNA-PKcs transcriptional factor Sp1. DNA-PK protein/mRNA Sp1 significantly higher the tumor compared with tissues. Significant correlations between observed. Because Ku80 have consensus recognition elements their promoter region, sequence Ku70 region was...
Hypoxic cells exhibit radioresistance, which is associated with poor prognosis in cancer patients. Understanding the molecular mechanisms underlying radioresistance hypoxic tumor crucial for improving radiotherapy efficacy. In this study, we examined role of FDX1 regulating cellular responses to severe hypoxia glioblastoma cell lines T98G and A172. We found that expression was upregulated under hypoxia, its knockdown reduced hypoxia-induced activation key factors survival mechanisms,...
Preoperative radiotherapy reduces the rate of local recurrence and improves chance survival in patients with resectable, advanced rectal carcinoma. However, because not all tumors respond similarly to radiation, sorting out suitable is required irradiate rationally. The authors examined possible role Ku protein determining tumor radiosensitivity disease free carcinoma.The studied 96 In preradiation biopsy specimens samples, number cells that were stained positive for was evaluated by...
ATM and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) have been shown to sequences homologous the domains of mammalian phosphatidylinositol 3-kinase (PI3-kinase). In order determine contribution DNA-PKcs increased sensitivity cells DNA-damaging agents observed in presence PI3-kinase inhibitors, we examined effects a inhibitor, wortmannin, on cellular bleomycin (BLM), mitomycin C (MMC), X-irradiation ultraviolet (UV)-irradiation using 2 human tumor cell lines (T98G A172),...
The DNA double-strand breaks (DSBs) repair pathway has been implicated in maintaining genomic integrity via suppression of chromosomal rearrangements. DNA-dependent protein kinase (DNA-PK) an important role with DSBs repair. In this study, 93 untreated cancer patients and 41 cancer-free healthy volunteers were enrolled. Peripheral blood was collected, separated centrifuged; DNA-PK activity measured by DNA-pull-down assay. expressions DNA-PKcs, Ku70 Ku86 examined RT–PCR assay western...
Abstract Sodium orthovanadate (vanadate) inhibits the DNA-binding activity of p53, but its precise effects on p53 function have not been examined. Here, we show that vanadate exerts a potent antiapoptotic through both transcription-dependent and transcription-independent mechanisms relative to other inhibitors, including pifithrin (PFT) α. We compared PFTα PFTμ, an inhibitor apoptosis by p53. Vanadate suppressed p53-associated apoptotic events at mitochondria, loss mitochondrial membrane...
The International Commission on Radiological Protection has lowered the annual equivalent eye-lens dose to 20 mSv. Although occupational exposure can be high in nuclear medicine (NM) departments, few studies have been conducted regarding among NM staff. This study aimed estimate lens doses of staff an department and identify factors contributing exposure. Four nurses six radiographers performing positron emission tomography (PET) examinations four radioisotope (RI) (excluding PET) were...
We have investigated whether low-dose total-body X irradiation could suppress the development of lymphoma in AKR mice. Male mice were irradiated with 5 cGy three times a week or 15 two from 11 weeks age for 40 weeks. The incidences 80.5% sham-irradiated mice, 67.5% and 48.6% twice week. Incidence was significantly reduced by (P = 0.006). mean survival time prolonged 283 +/- 3 days control to 316 10 0.008) 309 14 0.040). Because about 80% male die lymphoma, it is likely that life span...
Abstract We have previously reported the antimetastatic effects and augmentation of immune responses, which would be a mechanism effects, 0.1 to 0.2 Gy total body irradiation. To elucidate cellular mechanisms response, we investigated low‐dose irradiation on gene expression interleukin‐1β (IL‐1β) IL‐6 using mouse peritoneal macrophages in vitro. Absolute mRNA quantification was carried out competitive polymerase chain reaction. Gene IL‐1β increased 1 2 hr after 2.0 then decreased below basal...
We report the p35 and p60 forms of XRCC4 protein, appearing in human leukemia MOLT‐4 or U937 cells following X‐irradiation hyperthermia. appeared conjunction with cleavage DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) fragmentation internucleosomal DNA, was suppressed by Ac‐DEVD‐CHO. also produced vitro treating cell lysate recombinant caspases, suggesting that a caspase‐cleaved fragment apoptotic death. sensitive to treatment phosphatase wortmannin undetectable M059J deficient...
Esophageal cancer tissues and adjacent normal mucosae in 13 patients with primary esophageal were examined for quantitative differences DNA-dependent protein kinase (DNA-PK) activity expressions of Ku70, Ku80 DNA-PKcs proteins by Western blotting immunohistochemistry. The tumor showed higher DNA-PK than the mucosae. Protein levels correlated activities tissues. Immunohistochemical analysis revealed that located predominantly nuclei both In epithelium, expressed only basal cell layers not...
DTNBP1 has been recognized as a schizophrenia susceptible gene, and its protein product, dysbindin-1, is down-regulated in the brains of schizophrenic patients. However, little known about physiological role dysbindin-1 central nervous system. We hypothesized that disruption with unidentified proteins could contribute to pathogenesis symptoms schizophrenia. GST pull-down from human neuroblastoma lysates showed an association DNA-dependent kinase (DNA-PK) complex. The DNA-PK complex interacts...