A5067133936- Advanced MRI Techniques and Applications
- Trace Elements in Health
- Advanced Neuroimaging Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- Liver Disease and Transplantation
- Organ Transplantation Techniques and Outcomes
- Ultrasound and Hyperthermia Applications
- Salivary Gland Disorders and Functions
- Neuroscience and Neuropharmacology Research
- Nanoparticle-Based Drug Delivery
- Diet and metabolism studies
- Lanthanide and Transition Metal Complexes
- Radiomics and Machine Learning in Medical Imaging
- RNA regulation and disease
- Neurological disorders and treatments
- Virus-based gene therapy research
- Biochemical effects in animals
- Drug Transport and Resistance Mechanisms
- Erythrocyte Function and Pathophysiology
- NMR spectroscopy and applications
- Advanced biosensing and bioanalysis techniques
- Iron Metabolism and Disorders
- Photoacoustic and Ultrasonic Imaging
King's College London
2015-2024
Wellcome Centre for Human Neuroimaging
2013-2019
Bansomdejchaopraya Rajabhat University
2019
Neuroscience Institute
2018
University of Westminster
2017
University of London
2016
Cognitive Neuroimaging Lab
2004-2015
University College London
1997-2015
University of Cambridge
2004-2014
British Heart Foundation
2014
Nuclear receptors and their coactivators have been shown to function as key regulators of adipose tissue biology. Here we show that a ligand-dependent transcriptional repressor for nuclear plays crucial role in regulating the balance between energy storage expenditure. Mice devoid corepressor protein RIP140 are lean, resistance high-fat diet-induced obesity hepatic steatosis, increased oxygen consumption. Although process adipogenesis is unaffected, expression certain lipogenic enzymes...
Iron dyshomeostasis is implicated in Alzheimer's disease (AD) alongside β-amyloid and tau pathologies. Despite the recent discovery of ferroptosis, an iron-dependent form cell death, hitherto, vivo evidence ferroptosis AD lacking. The present study uniquely adopts integrated multi-disciplinary approach, combining protein (Western blot) elemental analysis (total reflection X-ray fluorescence) with metabolomics (1H nuclear magnetic resonance spectroscopy) to identify iron possible novel...
Multiple sclerosis is an inflammatory, degenerative disease of the central nervous system. The most obvious pathological change in multiple multifocal demyelination white matter, but grey matter may be equal or even greater importance for its clinical manifestations. In order to assess pathogenetic role lesions and explore association between demyelinated non-lesional brain tissue, tools are needed depict each these tissue components accurately vivo. Due sensitivity detecting lesions,...
Graves' orbitopathy (GO) is a complication in disease (GD) but mechanistic insights into pathogenesis remain unresolved, hampered by lack of animal model. The TSH receptor (TSHR) and perhaps IGF-1 (IGF-1R) are considered relevant antigens. We show that genetic immunization human TSHR (hTSHR) A-subunit plasmid leads to extensive remodeling orbital tissue, recapitulating GO. Female BALB/c mice immunized with hTSHR or control plasmids vivo muscle electroporation were evaluated for...
Abdominal aortic aneurysms constitute a degenerative process in the wall. Both miR-29 and miR-15 families have been implicated regulating vascular extracellular matrix.Our aim was to assess effect of family on aneurysm development.Among members, miR-195 differentially expressed aortas apolipoprotein E-deficient mice angiotensin II infusion. Proteomics analysis secretome murine smooth muscle cells, after manipulation, revealed that targets cadre matrix proteins, including collagens,...
BackgroundAdipose tissue patterning has a major influence on the risk of developing chronic disease. Environmental influences both body fat and appetite regulation are not fully understood. This study was performed to investigate impact resistant starch (RS) adipose deposition central in mice.Methodology Principle FindingsForty mice were randomised diet supplemented with either high (HRS), or readily digestible (LRS). Using 1H magnetic resonance (MR) methods, whole adiposity,...
A novel bimodal fluorescent and paramagnetic liposome is described for cellular labeling. In this study, we show the synthesis of a gadolinium lipid, Gd.DOTA.DSA, designed liposomal cell labeling tumor imaging. Liposome formulations consisting lipid were optimized in order to allow maximum entry, formulation was used label HeLa cells vitro. The efficiency Gd-liposome demonstrated using both fluorescence microscopy magnetic resonance imaging (MRI). uptake Gd-liposomes into induced marked...
Obesity has become a major global health problem. Recently, attention focused on the benefits of fermentable carbohydrates modulating metabolism. Here, we take system approach to investigate physiological effects supplementation with oligofructose-enriched inulin (In). We hypothesize that this carbohydrate will not only lead changes in body weight and composition, but also modulation neuronal activation hypothalamus. Male C57BL/6 mice were maintained normal chow diet (control) or high fat...
Metabolic profiling by 1H NMR spectroscopy is an underutilized technology in salivary research, although preliminary studies have identified promising results multiple fields (diagnostics, nutrition, sports physiology). Translation of findings into validated, clinically approved knowledge hindered variability protocol for the collection, storage, preparation, and analysis saliva. This study aims to evaluate effects differing sample pretreatments on metabolic profile Protocol considerations...
A key challenge in medical imaging is determining a precise correspondence between image properties and tissue microstructure. This comparison hindered by disparate scales resolutions histology. We present new technique, 3D Bridging of Optically-clear histology with Neuroimaging Data (3D-BOND), for registering images to overcome these limitations. Ex vivo 120 × 200 μm resolution diffusion-MRI (dMRI) data was acquired at 7 T from adult C57Bl/6 mouse hippocampus. Tissue then optically cleared...
Salivary metabolomics is rapidly advancing.To determine the extent to which salivary metabolites reflects host or microbial metabolic activity whole-mouth saliva (WMS), parotid (PS) and plasma collected contemporaneously from healthy volunteers were analysed by 1H-NMR spectroscopy. Spectra underwent principal component analysis k-means cluster metabolite quantification. WMS samples cultured on both sucrose peptide-enriched media. Correlation between concentration bacterial load was...
Microglia and astrocytes can quench metal toxicity to maintain tissue homeostasis, but with age, increasing glial dystrophy alongside dyshomeostasis may predispose the aged brain acquire neurodegenerative diseases. The aim of present study was investigate age-related changes in deposition along distribution normal C57Bl/6J mice 2-, 6-, 19- 27-months (n = 4/age). Using synchrotron-based X-ray fluorescence elemental mapping, we demonstrated increases iron, copper, zinc basal ganglia (p <...
The anatomy of the substantia nigra on conventional MRI is controversial. Even using histological techniques it difficult to delineate with certainty from surrounding structures. We sought define SN high field spin-echo pathological material in which we could study detail corroborate our findings controls and Parkinson's disease progressive supranuclear palsy. 23 brains were selected Queen Square Brain Bank (10 controls, 8 palsy, 5 disease) imaged 9.4 Tesla MRI. Subsequently cut stained...
To measure regional T1 and T2 values for normal C57Bl/6 mouse brain changes in after systemic administration of manganese chloride (MnCl2) at 9.4 T.C57Bl/6 mice were anesthetized baseline measurements obtained prior to measurement MnCl2 T. was administered systemically either by the intravenous (IV), intraperitoneal (IP), or subcutaneous (SC) routes. maps each MRI transverse slice generated using commercial software, white matter (WM), gray (GM), pituitary gland, lateral ventricle...
This paper describes the development of a simple and straightforward approach for preparation matrix-matched calibration standards Fe quantitative tissue imaging by LA-ICP-MS.