A5075639257- Aquaculture disease management and microbiota
- Animal Genetics and Reproduction
- Aquaculture Nutrition and Growth
- Glycosylation and Glycoproteins Research
- Metabolism, Diabetes, and Cancer
- Physiological and biochemical adaptations
- Bacterial Genetics and Biotechnology
- Erythrocyte Function and Pathophysiology
- Meat and Animal Product Quality
- Viral Infectious Diseases and Gene Expression in Insects
- Amino Acid Enzymes and Metabolism
- Pancreatic function and diabetes
- Estrogen and related hormone effects
- Molecular Biology Techniques and Applications
- Genetic and phenotypic traits in livestock
- Reproductive System and Pregnancy
- Probiotics and Fermented Foods
- Reproductive biology and impacts on aquatic species
- Reproductive Physiology in Livestock
- Barrier Structure and Function Studies
- Infant Nutrition and Health
- Metabolism and Genetic Disorders
- Effects of Radiation Exposure
- Animal Nutrition and Physiology
- Photoreceptor and optogenetics research
Ollscoil na Gaillimhe – University of Galway
2007-2019
National University of Ireland
2004-2008
University of Ulster
1997-1999
Belfast City Hospital
1997-1999
University of Cambridge
1992-1997
Queen's University Belfast
1997
Queens University
1997
Addenbrooke's Hospital
1997
University of Leeds
1994
University of London
1987-1991
Antibodies were raised in rabbits against synthetic peptides corresponding to the N-terminal (residues 1-15) and C-terminal 477-492) regions of human erythrocyte glucose transporter.The antisera recognized intact transporter enzymelinked immunosorbent assays (ELISA) Western blots.In addition, anti-C-terminal peptide antibodies demonstrated, by competitive ELISA immunoadsorption experiments, bind native transporter.Competitive ELISA, using erythrocytes, unsealed membranes, or membrane...
Abstract Background Studies conducted with gilthead sea bream ( Sparus aurata L.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. The present study aimed to analyze effect nutritional background on health fed plant protein-based diets (FO diet) a blend vegetable oils (66VO were exposed for 102 days intestinal myxosporean parasite Enteromyxum leei , intestine transcriptome was analyzed customized oligo-microarray 7,500...
The ProP protein of Escherichia coli is an osmoregulatory H+-compatible solute cotransporter. activated by osmotic upshift in both whole cells and membrane vesicles. We are using biochemical biophysical techniques to explore the osmosensory catalytic mechanisms ProP. now report purification reconstitution active transporter. Protein was facilitated addition six histidine (His) codons 3' end proP. recombinant gene overexpressed from E. galP promoter, ProP−(His)6 shown be functionally...
Treatment of the purified, reconstituted, human erythrocyte glucose transporter with trypsin lowered its affinity for cytochalasin B more than 2-fold, and produced two large, membrane-bound fragments. The smaller fragment (apparent Mr 18000) ran as a sharp band on sodium dodecyl sulphate (SDS)/polyacrylamide-gel electrophoresis. When was photoaffinity labelled [4-3H]cytochalasin before tryptic digestion, this became radiolabelled so probably comprises part binding site, which is known to lie...
Abstract Background Selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. The gilthead sea bream ( Sparus aurata ) is main aquacultured species Mediterranean area there considerable interest genetic this species. With aim increasing genomic resources identifying genes mechanisms underlying physiology response, we developed a cDNA microarray that enriched by suppression substractive hybridization with immunorelevant...
Our understanding of the mechanisms underlying stress responses in fish remains fragmentary. However, new insights into these and their biological significance have been provided by investigation at transcriptional level. Microarray technology has allowed unbiased analysis transcriptome, providing a potentially system-wide overview responses. In this review, we present recently published transcriptomic studies on exposed to range environmental, xenobiotic, social, aquacultural stressors....
The primary element in the cAMP signal transduction pathway is cAMP-dependent protein kinase (PKA). Expression of RIα subunit type I PKA elevated a variety human tumours and cancer cell lines. purpose this study was to assess prognostic importance expression patients with ovarian cancer. We have evaluated panel (n= 40) five lines using quantitative reverse transcription polymerase chain reaction (RT-PCR) Western blot analysis. OAW42 OTN14 express high endogenous levels mRNA (at significantly...
The glucose-transport protein from bovine cerebral-cortex microvessels has been identified and characterized by virtue of its ability to bind the ligand [4-3H]cytochalasin B. Microvessel membranes were found contain a single set glucose-inhibitable high-affinity cytochalasin B-binding sites [113 +/- 16 (S.E.M.) pmol/mg membrane protein], with an association constant 6.8 1.8 micron-1. D-Glucose inhibited binding these Ki 31 mM. transport was photoaffinity labelling B migrate as broad band...
Increasing evidence indicates an association between the concentration of systemic progesterone during early luteal phase oestrous cycle and embryo survival rate in cattle. We examined relationship on Days 4 to 8 post-ovulation expression receptor (PGR), oestrogen α (ESR1) retinol-binding protein (RBP) mRNA bovine endometrium. Heifers were blood sampled from day ovulation (Day 0) Day post-ovulation. On 4, animals divided into low control (LC) high (HC) groups based their plasma...
Abstract Background The male Atlantic salmon generally matures in fresh water upon returning after one or several years at sea. Some fast-growing parr develop an alternative life strategy where they sexually mature before migrating to the oceans. These so called 'precocious' 'sneakers' can successfully fertilise adult female eggs and perpetuate their line. We have used a custom-built cDNA microarray investigate gene expression changes occurring gonad brain associated with precocious...
The bystander response has been documented in cell lines and cultures derived from aquatic species over the past several years. However, little work undertaken to identify a similar tissue explant fish. In this study, indirect effects of ionizing gamma radiation on fish were investigated. Tissue explants culture exposed 0.5 Gy 5 60Co teletherapy unit. A Epithelioma papulosum cyprini (EPC) cells gamma-irradiated conditioned medium rainbow trout was investigated, survival quantified by...
To identify glycosylation-related genes in the HT29 derivative cell line, HT29-MTX-E12, showing differential expression on infection with
Cytochalasin B is a potent inhibitor of mammalian passive glucose transporters. The recent demonstration sequence similarities between these proteins and several bacterial proton-linked sugar transporters suggested that cytochalasin might be useful tool for investigation the galactose/H+ symport protein (GalP) Escherichia coli. Equilibrium binding studies using membranes from GalP-constitutive (GalPc) strain E. coli revealed single set high affinity sites with Kd 0.8-2.2 microM. Binding was...
The technique of photoaffinity labelling with [4-3H]cytochalasin B was applied to osmotically lysed cerebral microvessels isolated from sheep brain. Cytochalasin photo-incorporated into a membrane protein average apparent Mr 53,000. Incorporation cytochalasin inhibited by D-glucose, but not L-glucose, which strongly suggests that the labelled is, or is component of, glucose transporter blood-brain barrier. Investigation noncovalent binding equilibrium dialysis indicated presence single set...
O'Dowd, C., Mothersill, C. E., Cairns, M. T., Austin, B., Lyng, F. M., McClean, Talbot, A. and Murphy, J. E. Gene Expression Enzyme Activity of Mitochondrial Proteins in Irradiated Rainbow Trout (Oncorhynchus Mykiss, Walbaum) Tissues In Vitro. Radiat. Res. 171, 464–473 (2009).In recent years ethical, legislative economic pressures have created a renewed interest the development alternatives to vivo animal experiments. vitro studies, particularly those using cell cultures, been used...