A5069497839- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Nanoplatforms for cancer theranostics
- Advanced Proteomics Techniques and Applications
- Phagocytosis and Immune Regulation
- Antimicrobial Resistance in Staphylococcus
- Advanced Biosensing Techniques and Applications
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Immune cells in cancer
- Protein Degradation and Inhibitors
- Biosensors and Analytical Detection
- Cancer Genomics and Diagnostics
- Nanoparticle-Based Drug Delivery
Karolinska Institutet
2023
University of Miami
2020-2022
Sylvester Comprehensive Cancer Center
2020
Interface (United States)
2010-2014
University of Florida Health
2010-2014
University of Florida
2009-2012
Background Ovarian cancer is the most lethal gynecological malignancy, and ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in outcomes, especially for OCCA, could be expected from clearer understanding of molecular pathology that might guide strategies earlier diagnosis more effective Methodology/Principal Findings Cell-SELEX technology was employed develop new probes surface markers. A total thirteen aptamers with...
In this paper, we describe the elucidation of target an aptamer against ovarian cancer previously obtained by cell-SELEX (SELEX = systematic evolution ligands exponential enrichment). The target's identity, stress-induced phosphoprotein 1 (STIP1), was determined mass spectrometry and validated flow cytometry, using siRNA silencing protein blotting. Initial oncologic studies show that inhibits cell invasion, indicating STIP1, which is currently under investigation as a potential biomarker for...
Local delivery of anticancer agents has the potential to maximize treatment efficacy and minimize acute long-term systemic toxicities. Here, we used unsupervised systematic evolution ligands by exponential enrichment identify four RNA aptamers that specifically recognized mouse human myeloid cells infiltrating tumors but not their peripheral or circulating counterparts in multiple models from patients with head neck squamous cell carcinoma (HNSCC). The use these conjugated doxorubicin...
To generate DNA-aptamers binding to Methicillin-resistant Staphylococcus aureus (MRSA).The Cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technology was used run the selection against MRSA bacteria and develop target-specific aptamers. were targeted while Enterococcus faecalis for counter during that process. Binding assays determine right aptamer candidates as well on clinical samples performed through flow cytometry analyzed using FlowJo software. The...
Background Obesity has reached epidemic proportions, affecting more than one tenth of the world’s population. As such, adipose tissue is being increasingly recognized as an important therapeutic target for obesity and related metabolic disorders. While many potential targets have been established drugs developed, very few those specifically without other tissue. This results from a limited knowledge both cell-surface markers physicochemical traits specific to adipocytes that might otherwise...
The ability to detect and target β cells in vivo can substantially refine how diabetes is studied treated. However, the lack of specific probes still hampers a precise characterization human cell mass delivery therapeutics clinical settings. Here, we report identification two RNA aptamers that specifically selectively recognize mouse cells. putative targets are transmembrane p24 trafficking protein 6 (TMED6) clusterin (CLUS). When given systemically immune deficient mice, these islet graft...
Lung cancer is the most lethal malignancy in world, and each year thousands of people die from this disease. Early detection has proven to increase 5-year survival for general, independent origination site lung. To address challenge, we have used cell-based SELEX (Systematic Evolution Ligands by Exponential Enrichment) select a panel aptamers capable distinguishing lung adenocarcinoma cells normal epithelial cells. These bind at physiological formalin-fixed conditions display affinity their...
Introduction: Despite the remarkable progress made over past decades and important clinical results in transplanting autologous pancreatic islets, islet transplantation remains suboptimal terms of engraftment long-term survival. Islet is hampered by difficulties to reach sufficient mass ensure insulin independence, caused primary non-function against donor islets. An aggressive immune suppressive regime typically life-long administered recipient prevent rejection transplanted allogenic mass,...
Abstract Myeloid cells have been extensively studied in the tumor microenvironment for their role promoting cancer cell survival and metastases. However, peculiar activated phenotype has not exploited targeted delivery of anticancer agents to site. Here we report a new therapeutic strategy that allows drugs both metastases by preferential targeting genetically stable tumor-infiltrating myeloid (TIMC). We identified 4 monoclonal RNA aptamers specifically recognize myeloid-derived suppressor...