Evidence suggests that IL-6 affects bone mass by modulating osteocyte communication towards osteoclasts. However, the mechanism which enhances osteocyte-mediated osteoclastogenesis is unclear. We aimed to investigate inflammatory factors in serum after orthodontic surgery and their relationship between osteocytes osteoclasts.Serum was obtained from 10 orthognathic patients, were detected ELISA. treated osteocyte-like cell line MLO-Y4 with recombinant mouse receptor (IL-6R), used quantitative...

Wound dressing patches based on stem cells have demonstrated promising potential in the treatment of diabetic wounds, while their capabilities maintenance cell stemness, effective exchange cellular substance, and precise targeting remain to be promoted. Here, a novel spheroid loaded microneedle (MN@SPs) patch is presented using microfluidic templating technology. By leveraging fluid manipulation template, spheroids (SPs) with uniform size can generated situ. The resulting SPs exhibit...

Management of infected wounds has raised worldwide concerns. Attempts in this field focus on the development intelligent patches for improving wound healing. Here, inspired by cocktail treatment and combinational therapy stratagem, we present a novel Janus piezoelectric hydrogel patch via 3-dimensional printing sonodynamic bacteria elimination The top layer printed was poly(ethylene glycol) diacrylate with gold-nanoparticle-decorated tetragonal barium titanate encapsulation, which realizes...

The regenerative function of liver can be destroyed by viral infection, drug poisoning and tumorigenesis, resulting in irreversible damage. Numerous approaches promoting repair intend to replace transplantation, which is faced with a shortage donors. Owing the significant advantages cell programming bioscaffold engineering, tissue engineering considered most promising alternative for mimicking microstructure, maintaining hepatic or implanting whole liver. Cell sources gradually develop from...

Microparticles (MPs)-based delivery systems have a demonstrated value in gastrointestinal administration. Research this area is focusing on the development of multifunctional MPs to improve effects. Herein, learning from natural morphology peony pollens, novel multilobe for target surface adhesion and durable drug release are pesented. Ascribing flexibility microfluidic technology combination selective hydrogel degradation methods, with series structures can be obtained using alginate...

Abstract Mesenchymal stem cell (MSC) therapy is a promising candidate for inflammatory bowel disease (IBD) treatment, while overcoming the limitations of naive seeding cells function and realizing efficient intestinal targeting remains challenge. Here, bioadhesive microparticle carrying interleukin‐27 (IL‐27) MSC‐derived extracellular vesicles (MSC IL‐27 EVs) developed to treat IBD. The MSC EVs prepared through lentivirus‐mediated gene transfection technology show ideal anti‐inflammatory...

Exosomes-loaded hydrogels have potential value in wound treatment. Current studies focus on improving hydrogels' biocompatibility and optimizing different stem cell-derived exosomes for better therapeutic effect. Herein, we present a novel biocompatible recombinant human collagen (RHC) hydrogel loading with MSCs-derived promoting healing. We modify the RHC methacrylate anhydride (MA) at optimal concentration, generating (RHCMA) ideal physiochemical properties exosome delivery...

Abstract Mesenchymal stem cells (MSCs) have a demonstrated value for acute liver failure (ALF) regeneration, while their delivery stratagems with long‐term biological functions, low immune response, and high biocompatibility are still challenge. Here, lipopolysaccharide (LPS)‐loaded photoresponsive cryogel porous microcarrier (CPM) MSCs colonization is presented to promote defect regeneration. The CPMs fabricated graphene oxide, poly( N ‐isopropylacrylamide), gelatin methacrylate (GelMA) via...

Antibiotics provide promising strategies for treating periodontitis, while their delivery and controllable release with desired oral retention remain challenging. Here, inspired by the unique suction-cup structures of abalones, a novel adhesive photo-responsive microparticle (MP) system is developed to treat periodontitis through microfluidic electrospray technology. Such MPs are generated quickly ionic cross-linking sodium alginate together photo-curing poly(ethylene glycol) diacrylate...

The tumor-suppressing efficacy of either chemotherapeutics or gaseous drugs has been confirmed in treating the triple negative breast cancer (TNBC), while single treatment is usually dissatisfactory. Herein, a novel ultrasound responsive natural pollen delivery system presented to simultaneously load and for synergistic TNBC. hollow structure grains carries oxygen-enriched perfluorocarbon (PFC), porous spinous process adsorbs chemotherapeutic drug doxorubicin (DOX) (PO/D-PGs). Ultrasound can...

Abstract Reactive oxygen species (ROS) have certain effect in cancer treatment, thus many studies been focused on developing functional systems to generate ROS tumor. Here, inspired by the multi‐enzyme biocatalysis organisms, novel ultrasound‐triggered temporospatial catalytic cascades are presented based barium titanate (BTO) and platinum (Pt) co‐loaded multi‐component microparticles (Pt/BTO@MCMPs) successively achieve production for tumor sonodynamic therapy. By using a customized...

The purpose of this study is to investigate the possible mechanism and neuroprotective effect human urinary kallidinogenase (HUK) in cerebral ischemia. mouse middle artery occlusion (MCAO) model was used. Mice were treated with HUK (20 PNAU/g per day, intravenous) or saline as control, from beginning reperfusion 72 h. Neurological deficits, infarct size, BWC measured at 6, 24, 48, h after MCAO, respectively. Pathological changes brain observed by TUNEL assay. Inflammatory factors real-time...

Abstract Breast cancer is the most common type in women. Long non-coding RNAs (lncRNAs) have been reported as potential new diagnostic markers, prognostic factors, and therapeutic targets cancer. However, specific roles mechanisms of lncRNAs breast remain to be elucidated. Here we demonstrated downregulation lncRNA SNORD3A cells tissues verified its non-protein-coding property. overexpression had no effect on cell proliferation but specifically sensitized 5-fluorouracil (5-FU) vitro vivo....

Abstract Anticancer treatment is a worldwide challenge, and there are constant endeavors in this field to develop intelligent drug delivery systems improve the therapeutic effect. This paper presents novel ultrasound‐responsive microbubbles (UDMs), with chemotherapeutic‐encapsulated hydrogel shells gas cores. These developed using microfluidic electrospray treat pancreatic tumors. As composed of thermo‐sensitive poly(N‐isopropylacrylamide) (PNIPAM), they can contract under mild hyperthermia...

Premature ovarian failure (POF) features an upward incidence nowadays, and the human umbilical cord mesenchymal stem cells (hUC-MSCs)-derived exosomes (MSC-Exos) have shown applied values in recovery of function. Here, a novel exosome-encapsulated microcarrier prepared by microfluidic technology for repair after chemotherapy damage is presented. The derived from lipopolysaccharide (LPS)-preconditioned hUC-MSCs are encapsulated with hyaluronic acid methacryloyl (HAMA) via electrospray, which...

Engineered liver lobule is highly practical in hepatic disease treatment, while constructing a 3D biomimetic with heterogeneous architecture on large scale challenging. Here, inspired by the natural architectural construction of lobules, lobules are proposed coaxially through-pores for nutrient exchange via microfluidic technology. This multi-channel chip made parallelly installing capillaries. Sodium alginate (Alg) pumped through its central channel, Ca

Liver plays a critical role in drug metabolism and nowadays multi-cellular culture systems aiming at imitating liver-specific morphology functionality are advancing robustly. Numerous immortalized or stem cell-induced hepatic cell lines have been investigated to enhance the phenotype establishing co-culture systems. Owing robust progresses of microtechnology bioengineering, two-dimensional (2D) co-cultures such as sandwich micropatterned emerged. Controllably arranging hepatocytes...