A5052522957- DNA and Nucleic Acid Chemistry
- Genetics, Aging, and Longevity in Model Organisms
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- Circadian rhythm and melatonin
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Adipose Tissue and Metabolism
- Cancer therapeutics and mechanisms
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
- Mycotoxins in Agriculture and Food
- Hemoglobin structure and function
- Pregnancy and preeclampsia studies
- Zebrafish Biomedical Research Applications
- Fungal Plant Pathogen Control
- Endoplasmic Reticulum Stress and Disease
- Transgenic Plants and Applications
- Insect and Pesticide Research
- Stress Responses and Cortisol
- Chemical Reactions and Isotopes
- RNA modifications and cancer
- Birth, Development, and Health
- DNA Repair Mechanisms
Washington University in St. Louis
2023-2025
Kaohsiung Medical University
2018-2022
University of Michigan
1977-1982
W.E. Upjohn Institute for Employment Research
1982
Massachusetts Institute of Technology
1977
Center for Cancer Research
1977
University of California, Los Angeles
1977
ABSTRACT The renin-angiotensin-aldosterone system (RAAS) plays a well-characterized role regulating blood pressure in mammals. Pharmacological and genetic manipulation of the RAAS has been shown to extend lifespan Caenorhabditis elegans, Drosophila rodents, but its mechanism is not well defined. Here, we investigate angiotensin-converting enzyme (ACE) inhibitor drug captopril, which extends worms mice. To mechanism, performed forward screen for captopril-hypersensitive mutants. We identified...
Abstract Sodium is an essential nutrient, but toxic in excess. In humans, excessive dietary sodium can cause high blood pressure, which contributes to age-related diseases including stroke and heart disease. We used C. elegans elucidate how levels influence animal aging. Most experiments on this are conducted standard culture conditions: Nematode Growth Medium (NGM) agar with a lawn of E. coli . Here, we report that the supplemental NaCl NGM, 50 mM, accelerates aging decreases lifespan. For...
A preparation of bacteriophage T4-induced deoxyribonucleotide synthetase complex is described.This very large enzymes can be separated by centrifugation at 100,000 X g, sucrose step gradient centrifugation, or with molecular exclusion columns.By direct assay and unidimensional two-dimensional acrylamide electrophoretic separations the following T4-coded were shown to associated complex: ribonucleoside diphosphate reductase, dCMP deaminase, dCTP/dUTPase, hydroxymethylase, dTMP synthetase, DNA...
Kidney disease patients may have concurrent chronic kidney disease-associated mineral bone disorder and hypertension. Cardiovascular (CVD) neuropathy occur due to failure-induced accumulation of uremic toxins in the body. Indoxyl sulfate (IS), a product indole metabolism liver, is produced from tryptophan by intestinal flora ultimately excreted through kidneys. Hemodialysis helps renal failure eliminate many nephrotoxins, except for IS, which leads poor prognosis. Although impacts IS on...
Considerable progress has been made in understanding the synthesis of DNA even-numbered T phages. A T4 phage-induced polymerase demonstrated by Aposhian and Kornberg (1962), this enzyme shown to be encoded gene 43 deWaard et al. (1965) Warner Barnes (1966). Speyer (1966) have given evidence that is intimately connected with synthesis. In addition, ligase, an which implicated ϕX174 (Goulian al., 1967), induced infection phage (Weiss Richardson, 1967, Becker 30 its structural (Fareed 1967).
In earlier reports we have suggested that bacteriophate T4 DNA replication occurs in a complex composed of the proteins required for polymerization and system enzymes synthesizing deoxyribonucleoside triphosphate precursors DNA. T4-induced dCMP hydroxymethylase dTMP synthetase, though demonstrable extracts soon after infection, are not active vivo until about 5 min. The activities increase exponentially approximately 15 min then become constant. We exponential period represents formation...
Bacteriophage T4-infected Escherichia coli rendered permeable to nucleotides by sucrose plasmolysis exhibited two apparently separate pathways or channels T4 DNA with respect the utilization of exogenously supplied substrates. By one pathway, individual labeled ribonucleotides, thymidine (tdR), and 5-hydroxymethyl-dCMP could be incorporated into phage DNA. Incorporation each these compounds was not dependent upon addition other deoxyribonucleotide precursors, suggesting that a functioning de...
Unfolded protein response (UPR) is a cytoprotective mechanism that alleviates the protein-folding burden in eukaryotic organisms. Moderate activation of UPR required for maintaining endoplasmic reticulum (ER) homeostasis and profoundly contributes to tumorigenesis. Defects signaling are implicated attenuation various malignant phenotypes including cell proliferation, migration, invasion, as well angiogenesis. This suggests promising target cancer therapy. The pharmacological effects plant...
On infection by bacteriophage T4 mutants carrying lesions in the structural genes for ribonucleoside diphosphate reductase, nrdA or nrdB, rate of synthesis pyrimidine deoxyribonucleotides is reduced to about 25% that obtained with wild type phage.Previous studies from this laboratory have demonstrated proceeds at most phage genetically blocked DNA (Dna-), even though high levels accumulate (Tomich, P. K., Chiu, C.-s., Wovcha, M. G., and Greenberg, G. R. (1974) J. BioL Chem...
Abstract The renin-angiotensin-aldosterone system (RAAS) plays a well-characterized role regulating blood pressure in mammals. Pharmacological and genetic manipulation of the RAAS has been shown to extend lifespan C. elegans , Drosophila rodents, but its mechanism is not well defined. Here we investigate angiotensin-converting enzyme (ACE) inhibitor drug captopril, which extends worms mice. To mechanism, performed forward screen for captopril hypersensitive mutants. We identified missense...