A5109056670- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Vector-borne infectious diseases
- Parasites and Host Interactions
- Child Nutrition and Water Access
- Public Health and Social Inequalities
- Helminth infection and control
- Synthesis and Biological Evaluation
- Vitamin C and Antioxidants Research
- Parasite Biology and Host Interactions
- Anesthesia and Pain Management
- Pesticide Exposure and Toxicity
- Public Health and Environmental Issues
- Diphtheria, Corynebacterium, and Tetanus
- Agricultural and Food Production Studies
- Mosquito-borne diseases and control
- Pain Mechanisms and Treatments
- Health and Lifestyle Studies
- Parasitic Infections and Diagnostics
- Hemodynamic Monitoring and Therapy
- Coenzyme Q10 studies and effects
- Histiocytic Disorders and Treatments
- Forensic Entomology and Diptera Studies
- Neuropeptides and Animal Physiology
Universidad del Valle
2023
Murdoch University
2012-2020
Institución Universitaria Colegio Mayor de Antioquia
2015
Universidad de Antioquia
2001-2014
University of Chile
2009
While much is known of the impact trypanosomes on human and livestock health, in wildlife, although ubiquitous, have largely been considered to be non-pathogenic. We describe genetic diversity, tissue tropism potential pathogenicity naturally infecting Western Australian marsupials. Blood samples collected from 554 live-animals 250 extracted 50 carcasses sick-euthanized or road-killed animals, belonging 10 species marsupials, were screened for presence using a PCR 18S rDNA gene. results...
Abstract The precise guidelines recommended by the American Heart Association for blood pressure measurement are commonly overlooked health-care workers, who generally take in an arbitrary way. To validate this observation we designed a descriptive and observational study to be carried out major hospital. One hundred seventy-two workers divided into four groups (63 general practitioners, 25 clinical surgical specialists, 59 nurses) were evaluated two-part test. In first part (practical),...
We report the discovery of nontoxic fungicide fenarimol (1) as an inhibitor Trypanosoma cruzi (T. cruzi), causative agent Chagas disease, and results structure–activity investigations leading to potent analogues with low nM IC50s in a T. whole cell vitro assay. Lead compounds suppressed blood parasitemia virtually undetectable levels after once daily oral dosing mouse models infection. Compounds are chemically tractable, allowing rapid optimization target biological activity drug...
The trypanosome diversity of the Brush-tailed Bettong (Bettongia penicillata), known locally as woylie, has been further investigated. At a species level, woylies are critically endangered and have declined by 90% since 1999. predation individuals made more vulnerable disease is thought to be primary cause this decline, but remains proven. Woylies were sampled from three locations in southern Western Australia. Blood samples collected analysed using fluorescence situ hybridization,...
Chagas disease, caused by the protozoan parasite Trypanosoma cruzi (T. cruzi), is an increasing threat to global health. Available medicines were introduced over 40 years ago, have undesirable side effects, and give equivocal results of cure in chronic stage disease. We report development two compounds, 6 (S)-7, with PCR-confirmed curative activity a mouse model established T. infection after once daily oral dosing for 20 days at mg/kg 10 (S)-7. Compounds (S)-7 potent vitro activity, are...
A number of trypanosome isolates from Australian marsupials are within the clade containing human pathogen Trypanosoma cruzi. Trypanosomes this thought to have diverged a common ancestral bat trypanosome. Here, we characterise noyesi sp. nov. isolated critically endangered woylie (Bettongia pencillata) using phylogenetic inferences three gene regions (18S rDNA, gGAPDH, and CytB) coupled with morphological behavioural observations in vitro. We also investigated potential vectors presence T....
Introducción: malaria, parasitosis intestinales y desnutrición coexisten e interactúan en los habitantes de las zonas palúdicas; se requiere conocer la magnitud cada una esas enfermedades. Objetivo: identificar prevalencia parásitos niños (4–10 años) Urabá Bajo Cauca (Antioquia, Colombia) el cambio postratamiento antiparasitario intestinal específico. Metodología: con paludismo, hicieron encuestas parasitarias antes 8 30 días (albendazol + secnidazol). Se usaron coprológicos directo por...
Kinetoplast DNA (kDNA) is the mitochondrial genome of trypanosomatids. It consists a few dozen maxicircles and several thousand minicircles, all catenated topologically to form two-dimensional network. Minicircles are heterogeneous in size sequence among species. They present one or conserved regions that contain three highly blocks. CSB-1 (10 bp sequence) CSB-2 (8 lower interspecies homology, while CSB-3 (12 Universal Minicircle Sequence within most The located at replication origin binding...
This study attempted to evaluate the transmission dynamics of Trypanosoma cruzi in four indigenous communities Sierra Nevada de Santa Marta (SNSM), Colombia. Low-stringency single primer-polymerase chain reaction (LSSP-PCR) minicircles and Southern blot analyses were used characterize samples from patients, vectors, reservoirs these communities. The LSSP-PCR profiles revealed a high genetic variability but with similarities among parasites present same different Cluster analysis molecular...
Trypanosoma copemani has been found infecting several threatened/endangered marsupial species within Australia and is thought to be a key player in the rapid decline of woylie (Bettongia penicillata). To better understand biology life cycle this parasite, growth requirements, kinetics infection two newly described genotypes, T. G1 G2, were investigated compared with cruzi strain-10R26 vitro. Both G2 able infect all four cell lines tested. The number infected cells where at least one...
Trypanosomes are blood protozoan parasites that capable of producing illness in the vertebrate host. Within Australia, several native Trypanosoma species have been described infecting wildlife. However, only copemani has associated with pathological lesions wildlife hosts and more recently drastic decline critically endangered woylie (Bettongia penicillata). The impact some trypanosomes on health host led to development numerous drug compounds could inhibit growth or kill parasite. This...
Molecular and structural information on avian Trypanosoma spp. throughout Australia is limited despite their intrinsic value in understanding trypanosomatid evolution, diversity, biology. In Western tissue samples (n = 429) extracted from 93 birds 25 bird species were screened using generic PCR primers to investigate the diversity of To trypanosome biology first 3-dimensional ultrastructural models a (Trypanosoma sp. AAT) isolated (currawong, Strepera spp.) generated focussed ion beam...
Understanding the impacts of parasites on wildlife is growing in importance as diseases pose a threat to populations. Woylie (syn. brush-tailed bettong, Bettongia penicillata) populations have undergone enigmatic declines south-western Western Australia over past decade. Trypanosomes been suggested possible factor contributing towards these because their high prevalence declining population. We asked whether temporal patterns infection with Trypanosoma spp. were associated decline host, or...
Conventional nested PCR and Sanger sequencing methods are currently the gold standards for detecting trypanosomes in wildlife. However, these techniques time-consuming can often overlook mixed infections. True trypanosome prevalence thus be underrepresented. Here, we designed an 18S rDNA-based real-time quantitative (qPCR) assay coupled with High-Resolution Melting Analysis (HRMA) to detect discriminate three Trypanosoma species (T. copemani, T. noyesi, vegrandis) commonly infecting...
OBJETIVO GENERAL: estudiar el estado nutricional de los tules por medio la valoración antropométrica, adecuación en consumo nutrientes y determinación vitamina A sangre. METODOLOGÍA: es un estudio descriptivo, prospectivo, con encuestas transversales para detectar prevalencia alimentario riesgo desnutrición. Se hicieron alimentos, evaluación antropométrica medición retinol sangre (con HPLC). RESULTADOS: población del resguardo 2003 fueron 1.028 personas se evaluaron 791 (77%), 103 familias....
Objetivo: conocer la clase y frecuencia de las alteraciones mucocutáneas del cabello en niños zonas maláricas Antioquia (Colombia). Metodología: diseño experimental, balanceado, no ciego, con 93 paludismo, seguidos durante 30 días. Resultados: 61% los presentaron desnutrición crónica o global ambas según antropometría. Antes tratamiento hubo piel (41%), mucosa labial (12%), oral (2%), lengua (15%) (16%). El día mejoría todos estos sitios corporales, excepto (alteraciones: 42%). retinol...
La histiocitosis cefálica benigna es una enfermedad rara perteneciente a las cutáneas y mucocutáneas. El reconocimiento de esta patología importante para poder informar la familia del carácter benigno naturaleza auto resolutiva. Aunque no requiere tratamiento, realizar seguimiento evaluar evolución lesiones cutáneas. Se presenta el caso un paciente varón 2 años con pápulas amarillo-anaranjadas, asintomáticas, en rostro, sin sintomatología acompañante. realizó biopsia análisis histopatológico...
Problem: Studies on health/disease often use concepts such as equity/inequity, equality/inequality, and social justice. These terms are not always clearly precisely defined, nor they used with the same meaning by different authors. Objective: 1) To present analyze in literature living conditions, justice, health equity inequality, 2) to offer a conceptual framework that allows these be scientific manner. Methodology: Literature review comparison of both general specialist dictionaries,...