Acute gastroenteritis is the main cause of mortality and morbidity in children worldwide. Studies stated that rotavirus human adenovirus (HAdV) are common causes nonbacterial aged 0-5 years. The aim this study was to determine prevalence distribution rotavirus, HAdV, coinfections among hospitalized with below 7 years old enteric HAdV all gastroenteritis. conducted on 150 old. Antigen detection for by ELISA determination (serotype 40 41) nested PCR restriction endonucleases were performed....

Colistin is considered the last option for treatment of infections caused by Multidrug Resistant (MDR) Gram negative pathogens.The mcr-1 gene could transfer resistance to colistin between different species.Therefore, screening this mechanism will help greatly in control further spread and enhancing outcomes patients.The current work aimed study frequency among MDR pathogens isolated from clinical samples at Ain Shams University Hospitals explore if was responsible resistance.These were...

Human parechovirus (HPeV) has emerged as a pathogen associated with acute gastroenteritis (AGE).To detect the presence of HPeV in stool samples from Egyptian children AGE seeking care and possibility its co-infection other enteric viruses.One hundred were collected attending Mansoura University Children's Hospital AGE. astrovirus detected by reverse transcriptase-polymerase chain reaction (RT-PCR). At same time, detection rotavirus antigen norovirus was achieved enzyme-linked immunosorbent...

Abstract Urinary bladder cancer is a common malignancy in Egypt, thus reliable methodologies are required for screening and early detection. In this study, we analyzed the gene expression of Schistosoma hematobium specific microRNA “ Sha-miR-71a ” mitogen-associated protein kinase-3 (MAPK-3) urine samples 50 patients with benign bilharzial cystitis. Fifty control subjects were also tested. Indirect hemagglutination test (IHA) diagnosed 70% studied cases as associated (BBC), while...

Multiplex PCR is a sensitive and rapid method compared with conventional methods. Therefore, we use multiplex for the detection of four major intestinal pathogens causing gastroenteritis (Shigella spp., Campylobacter Aeromonas spp. Enterohemorrhagic Escherichia coli [EHEC]) in stool specimens.A prospective randomized study using 200 samples obtained from patients presented acute during period (between February 2019 December 2021). Bacteria were identified culture methods samples.The...

Abstract Objective Was to evaluate effect of clove, turmeric and garlic nano-herbal extracts on surface roughness microhardness demineralized dentin, their bactericidal Streptococcus mutans sobrinus with or without diode laser irradiation. Methods Three 5% nano-formulas were prepared characterized using transmission electron microscope. MI paste Plus™ was used as control. A total 100 specimens dentin treated 3 W-power laser; then, the different tested materials for 10-min before Vickers...

Abstract Background/Aims Today, the using of diode lasers in dentistry has made a significant progress; it increased speed treatment, decreased time healing, and showed bactericidal effect. The thermal effects should be considered root canal treatment by laser, as temperature rises to critical levels, causing tissues damage any change occurs after laser irradiation. can rise up 10 °C above body for less than 2–3 min without damaging periodontal tissue or burring tooth structure cooling....

Background: Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen causing life threatening infections.There are different determinants of virulence for these phenotypes like biofilm formation, large capsule, and siderophores.Many genes regulate as rmpA rmpA2.Aim the work: This study aims to detect antimicrobial resistance profile, association between with hypermucoviscosity formation among hypermucoviscous K. (hmvKp) isolates.Material Methods: Eighty (K.pneumoniae) isolates were...

Healthcare-associated urinary tract infection (UTI) represents a significant health problem, especially in infants and young children. The most common pathogen associated with this is Escherichia coli (E. coli).The present study aimed to detect the frequency of virulence genes among clinical isolates E. isolated from healthcare-associated infections children correlation between these presence blaCTX gene.The included one hundred healthcareassociated intensive care units. were subjected...

H1N1 infection in diabetic patients is of special concern and serious interest since the virus can place individuals, especially children, at great possible risk subsequently developing type 1 diabetes. This work aims to describe demographic characteristics, clinical features, severity illness children with diabetes mellitus (DM), compare incidence pandemic that general pediatric population influenza-like symptoms, identify complications associated glycemic control.The present study included...

Background Increasing frequency of blaOXA-48 gene, commonly identified in Enterobacteriaceae family, is alarming across the world. The poor hydrolytic profile OXA-48 likely may obscure its detection clinical isolates, complicating treatment option. Objective To detect prevalence gene carbapenem-resistant (CRE) isolates from patients Al-Zahraa and Helwan University hospitals, Cairo, Egypt, to compare diagnostic performance phenotypic tests for -mediated carbapenem resistance. Patients methods...

Background & Objective:: Our goal was to compare the in vitro activities of ceftazidime/avibactam (CZA) those observed response other drugs used treat carbapenem-resistant Enterobacteriaceae (CRE). Methods:: Bacterial isolates collected from intensive care unit patients with hospital-acquired infections were identified using VITEK 2TMCompact 15. Susceptibility testing for Escherichia coli and Klebsiella spp. performed Kirby- Bauer disk diffusion VITEK2 system. Minimum inhibitory...

Aim: To clinically evaluate the effect of continuous ultrasonic and diode laser 810 wave length irrigation activation techniques on postoperative pain bacterial reduction in single visit endodontic treatment mandibular molars.
 Material methods: Forty patients requiring root canal therapy for necrotic molars teeth were included this study with age ranges between 20 45 years. In all cases, was carried out using Revo-S rotary file system crown down sequence. NaOCl 2.5% solution used...

In recent years, the prevalence of infections with multidrug resistant Enterobacteriaceae has steadily increased. producing AmpC β-lactamases (AmpCs) have become a major therapeutic challenge. The detection AmpC-producing Proteus mirabilis is significant clinical relevance, as this may lead to inappropriate antimicrobial regimens and failure. aim study evaluate comparing routinely phenotypic methods in resistance molecular methods. From study, it can be concluded that cephamycin-Hodge test...

Escherichia coli (E. coli) is a common cause of nosocomial sepsis. There are multiple factors related to the severity sepsis among these presence virulence genes and pattern antibiotics resistance. The aim present study was determine prevalence pap gene encoding for pili, hlyA α-hemolysin cnf1 cytotoxic necrotizing factor 1 E. isolated from children with Also, correlate ESBL carbapenem resistance genes. retrospective cross-sectional included 150 non-duplicate strains blood cultures were...

Background. The goals of this study were to elucidate the use expression microRNA-30e (miR-30e) and microRNA-223 (miR-223) as diagnostic biomarkers for early diagnosis hepatocellular carcinoma (HCC) associated with hepatitis C virus (HCV) infection. Methods. included three groups, first group thirty patients HCC HCV, second cirrhosis HCV third healthy control subjects. Blood samples obtained determination serum miR-30e miR-223 by real time polymerase chain reaction. Results. There was...

Abstract Aim: Dysbiosis of gut microbiota favors chronic hepatic inflammation with subsequent carcinogenesis. The current study aimed to evaluate the role dysbiosis in development hepatocellular carcinoma patients HCV infection. Methods: This descriptive cross-sectional cohort carried out on 400 subjects recruited from Internal Medicine Department and Tropical Gastroenterology Helwan South Valley University Hospitals Egypt. period was January 2017 till 2020. were divided clinically into...

Human papilloma virus (HPV) related cervical cancer (CC) remains a significant cause of mortality, especially in developing nations.Practical, cheap, and responsive biomarkers are required to diagnose prevent large-scale CCs other cancers associated with HPV.This research aimed investigate HPV-Human leukocyte Antigen-G (HLA-G) interaction the immune modulation: Hypothesizing HPV-specific HLA markers might be accurate costeffective preventive biomarkers.In addition assessing host response by...