A5016926284- Monoclonal and Polyclonal Antibodies Research
- Drug-Induced Adverse Reactions
- Immunodeficiency and Autoimmune Disorders
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Autoimmune Bullous Skin Diseases
- Biosimilars and Bioanalytical Methods
- Systemic Sclerosis and Related Diseases
- Ovarian function and disorders
- Multiple Sclerosis Research Studies
- Mast cells and histamine
- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Reproductive Biology and Fertility
- Chronic Lymphocytic Leukemia Research
- IL-33, ST2, and ILC Pathways
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- Microscopic Colitis
- Urticaria and Related Conditions
- Pharmaceutical studies and practices
- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Sphingolipid Metabolism and Signaling
- ATP Synthase and ATPases Research
Hospital Universitario Austral
2025
Azienda Ospedaliero-Universitaria Careggi
1995-2024
University of Florence
2013-2023
European Centre for Theoretical Studies in Nuclear Physics and Related Areas
2015
Hospital Materno-Infantil
2012
To cite this article: Vultaggio A, Matucci Nencini F, Pratesi S, Parronchi P, Rossi O, Romagnani Maggi E. Anti‐infliximab IgE and non‐IgE antibodies induction of infusion‐related severe anaphylactic reactions. Allergy 2010; 65 : 657–661. Abstract Background: Infliximab is a chimeric monoclonal antibody against TNF‐α useful in the treatment many chronic inflammatory diseases. Severe anaphylaxis has been reported during therapy, although exact mechanism not fully defined. The reactions have...
Summary Background The administration of biological agents is potentially affected by IgE‐mediated infusion reactions. Objective aim the study was to evaluate utility skin testing in patients who have experienced infliximab ( IFX )‐related Methods Thirty with previous immediate hypersensitivity reaction , 20 disease‐matched non exposed subjects, 15 ‐treated tolerant and non‐responder were enrolled. Non‐isotype‐specific IgE anti‐drug antibodies ADA s) measured a double‐capture ELISA kit...
Rituximab (RTX) is currently used in the treatment of lymphoproliferative diseases and several rheumatologic disorders a frequent cause acute infusion reactions, usually classified as cytokine release syndrome (CRS). Some reactions to RTX raise concern for immediate type I hypersensitivity, even if date RTX-specific IgE antibodies have not been reported. To improve knowledge mechanisms RTX, we investigated humoral cellular immune responses this drug patient suffering from rheumatoid...
The mechanisms underlying endothelial cell injury and defective vascular repair in systemic sclerosis (SSc) remain unclear. Since the recently discovered angiogenic T cells (Tang) may have an important role of damaged endothelium, this study aimed to analyze Tang population relation disease-related peripheral features SSc patients. (CD3+CD31+CXCR4+) were quantified by flow cytometry blood samples from 39 patients 18 healthy controls (HC). Circulating levels CXCR4 ligand stromal cell-derived...
Immunogenicity of biological agents leads to the development antidrug antibodies (ADA) and it may be associated hypersensitivity reactions. Immediate infusion reactions occur during or within 1 h after infusion, their clinical manifestations vary considerably, ranging from mild severe life-threatening. Recent studies show that different mechanisms sustain toward biologics, application novel methods for detecting ADA has demonstrated involvement specific IgE isotypes. Considering severity...
Summary Antibodies recognizing infliximab (IFX) may develop in a proportion of treated patients, leading to loss response or hypersensitivity reactions (HRs). T cell IFX has been poorly investigated. This paper was addressed detect IFX-specific cells patients with inflammatory diseases developing, not, anti-drug antibodies (ADA) and correlate the presence specific clinical outcomes treatment. A co-culture system IFX-loaded dendritic purified autologous CD4+ used memory 32 ADA+ 39 ADA–...
Abstract This study evaluates the ability of a novel TLR7 ligand (9-benzyl-2-butoxy-8-hydroxy adenine, called SA-2) to affect IL-17 response. The SA-2 activity on expression IL-17A and IL-17–related molecules was evaluated in acute chronic models asthma as well vivo vitro α-galactosyl ceramide (α-GalCer)-driven systems. prepriming reduced neutrophils bronchoalveolar lavage fluid decreased methacoline-induced airway hyperresponsiveness murine models. These results were associated with...
Human gastric autoimmunity [autoimmune gastritis (AIG)] is characterized by inflammation of the mucosa and parietal cell loss. The proton pump H + /K -adenosine triphosphatase (H -ATPase) major autoantigen in AIG. Our work aimed to investigate -ATPase-specific T helper 17 (Th17) responses AIG serum interleukin (IL)-17 cytokine subfamily patients, healthy subjects [healthy controls (HCs)], patients with iron deficiency anemia (IDA) without We analyzed activation lamina propria mononuclear...
Mitochondrial disorders are devastating genetic diseases for which efficacious therapies still an unmet need. Recent studies report that increased availability of intracellular NAD obtained by inhibition the NAD-consuming enzyme poly(ADP-ribose) polymerase (PARP)-1 or supplementation with NAD-precursor nicotinamide riboside (NR) ameliorates energetic derangement and symptoms in mouse models mitochondrial disorders. Whether these pharmacological approaches also improve bioenergetics human...
IL-17A is associated with different asthma phenotypes as virus-associated or steroid-resistant asthma. Invariant natural killer T (iNKT) cells play an important role in the pathogenesis of The aim study was to evaluate activity polyinosinic-polycytidylic acid [poly(I:C)] on production by CD1d-activated iNKT cells.We analysed vitro effect poly(I:C) release spleen and lung α-galactosylceramide (α-GalCer). Its also investigated α-GalCer-induced murine models, including inflammation. inhibition...
Biological agents target disease mechanisms and have modified the natural history of several immune-mediated disorders. are structurally immunogenic, therefore usually elicit a minor, subclinical transient phenomenon. Occasionally, however, these drugs induce complete cellular humoral immune responses, with main clinical consequences being hypersensitivity reactions or loss treatment response. This article considers relative pathogenic influencing immunogenicity in biological discusses...
Pernicious anemia (PA) is a megaloblastic consisting of hematological, gastric and immunological alterations. The immunopathogenesis PA sustained by both autoantibodies (e.g. intrinsic factor (IFA) antibodies anti parietal cell (PCA) autoreactive T cells specific for IFA the proton pump ATPase. Iron deficient (IDA) microcytic represents most common cause worldwide. Our work aimed to investigate serum levels several interleukins (IL) IL-20 cytokine subfamily in patients with PA, IDA healthy...
Gleich’s syndrome (GS) is characterized by recurrent episodes of angioedema, increase in body weight, fever, hypereosinophilia and elevated serum IgM. The exact etiology remains unclear. Currently, the only treatment strategy administration high dose steroids during acute phases. We report case a 37-year-old man suffering from GS with [6000/mmc (45%)] eosinophil cationic protein (ECP) (>200 mcg/l), treated oral phase (prednisone 50-75 mg/day), maintenance being 25 mg/day. No monoclonal...
Abstract Infliximab (IFX) is a chimeric mAb that can lead to the appearance of anti-drug Abs. Recent research has identified presence circulating IFX-specific T cells in treated patients. The aim study was analyze functional characteristics cells, particular their capability produce biologically active regulatory cytokines. Drug-stimulated PBMCs or coculture systems were used detect memory cytokines produced by cell lines, and clones evaluated at mRNA protein levels. Drug infusion induced an...
This study aimed to evaluate the proportion of infliximab (IFX)-exposed patients exhibiting cellular response drug in a longitudinal way and establish whether it is predictive for anti-drug antibodies (ADA) development. Seventeen suffering from immuno-mediated disorders were enrolled. Blood was sampled at baseline before each first eight infusions IFX. The proliferation PBMCs 15-mer peptides covering VH/VL frames IFX assessed as well transcription factors cytokines mRNA expression memory T...