A5026471672- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Congenital heart defects research
- Cardiac electrophysiology and arrhythmias
- Nuclear Structure and Function
- Congenital Heart Disease Studies
- Risk Management in Financial Firms
- Peptidase Inhibition and Analysis
- Cardiovascular Function and Risk Factors
- Molecular Junctions and Nanostructures
- Advanced Memory and Neural Computing
- Cardiac Fibrosis and Remodeling
- Ubiquitin and proteasome pathways
- Tissue Engineering and Regenerative Medicine
- Protease and Inhibitor Mechanisms
- Retinal Development and Disorders
- Genetic and Kidney Cyst Diseases
- Reliability and Maintenance Optimization
- Semiconductor materials and devices
- Lysosomal Storage Disorders Research
- Pancreatic function and diabetes
- RNA regulation and disease
- Cardiomyopathy and Myosin Studies
University of Wisconsin–Madison
2019-2025
University of Notre Dame
2017
Cancer Research Institute
2017
Coat protein complex II (COPII) plays an integral role in the packaging of secretory cargoes within membrane-enclosed transport carriers that leave endoplasmic reticulum (ER) from discrete subdomains. Lipid bilayer remodeling necessary for this process is driven initially by membrane penetration mediated Sar1 GTPase and further stabilized assembly a multilayered several COPII proteins. However, relative contributions these distinct factors to carrier formation trafficking remain unclear....
Abstract Mechanisms underlying heterotypic subunit assembly of ion channels and other oligomeric assemblies are poorly understood. In the human heart, heteromeric two isoforms encoded by ether-à-go-go related gene ( hERG ) is essential for normal function cardiac I Kr in ventricular repolarization, with loss hERG1b contributing to arrhythmias associated long QT-syndrome. While hERG1a homomers traffic efficiently plasma membrane, retained endoplasmic reticulum (ER). When expressed together,...
Sympathetic innervation influences homeostasis, repair, and pathology in the cardiac ventricles; contrast, parasympathetic is considered to have minimal contribution influence ventricles. Here, we use genetic models, whole-mount imaging, three-dimensional modeling define nerve architecture during development, disease, regeneration. Our approach reveals that nerves extensively innervate Furthermore, identify sympathetic axons develop synchronously are bundled throughout We further investigate...
Significance The formation of multivesicular endosomes (MVEs) enables the sequestration numerous membrane-associated cargo molecules, including signaling receptors, to downregulate signal transduction pathways initiated by various stimuli. In this work, we demonstrate that machinery responsible for MVE biogenesis responds growth factor stimulation extending its duration action at endosomes. Specifically, show endosomal sorting complex required transport-I (ESCRT-I) and Vps4 accumulation MVEs...
Membrane type 1 matrix metalloproteinase (MT1-MMP, MMP-14) is a transmembrane collagenase highly expressed in metastatic ovarian cancer and correlates with poor survival. Accumulating evidence shows that the cytoplasmic tail of MT1-MMP subjected to phosphorylation, this post-translational modification regulates enzymatic activity at cell surface. To investigate potential role residue Thr567 phosphorylation regulation metastasis-associated behaviors, cells express low endogenous levels were...
Co-assembly of the multilayered coat protein complex II (COPII) with Sar1 GTPase at subdomains endoplasmic reticulum (ER) enables secretory cargoes to be concentrated efficiently within nascent transport intermediates, which subsequently deliver their contents ER-Golgi intermediate compartments. Here, we define spatiotemporal accumulation native COPII subunits and ER under differing nutrient availability conditions using a combination CRISPR/Cas9-mediated genome editing live cell imaging....
Coat protein complex II (COPII) governs the initial steps of biosynthetic secretory transport from endoplasmic reticulum (ER), facilitating movement a wide variety cargoes. Here, we demonstrate that Trk-fused gene (TFG) regulates rate at which inner COPII coat proteins are concentrated ER subdomains. Specifically, in cells lacking TFG, GTPase-activating (GAP) Sec23 accumulates more rapidly budding sites on as compared with control cells, potentially altering normal timing GTP hydrolysis...
Abstract Coat protein complex II (COPII) plays an integral role in the packaging of secretory cargoes within membrane-enclosed transport carriers that leave endoplasmic reticulum (ER) from discrete membrane subdomains. Lipid bilayer remodeling necessary for this process is driven initially by penetration coat subunit Sar1 and further stabilized assembly a multi-layer several COPII proteins. However, relative contributions these distinct factors to carrier formation trafficking remain...
Cardiac nerves regulate neonatal mouse heart regeneration and are susceptible to pathological remodeling following adult injury. Understanding cardiac nerve can lead new strategies promote repair. Our current understanding of architecture has been limited two-dimensional analysis. Here, we use genetic models, whole-mount imaging, three-dimensional modeling tools define neurovascular association during development, disease, regeneration. results demonstrate that sequentially associate with...
Co-assembly of the multilayered coat protein complex II (COPII) with Sari GTPase at subdomains endoplasmic reticulum (ER) enables secretory cargoes to be concentrated efficiently within nascent transport intermediates, which subsequently deliver their contents ER-Golgi intermediate compartments. Here, we define spatiotemporal accumulation native COPII subunits and ER under differing nutrient availability conditions using a combination CRISPR/Cas9-mediated genome editing live cell imaging....
SummaryCoat protein complex II (COPII) plays an integral role in the packaging of secretory cargoes within membrane-enclosed transport carriers that leave endoplasmic reticulum (ER) from discrete membrane subdomains. Lipid bilayer remodeling necessary for this process is driven initially by penetration coat subunit Sar1 and further stabilized assembly a multi-layer several COPII proteins. However, relative contributions these distinct factors to carrier formation trafficking remain unclear....