Ovarian fibrosis is a pathological condition associated with aging and responsible for variety of ovarian dysfunctions. Given the known contributions tissue to tumorigenesis, it anticipated that may contribute cancer risk. We recently reported diabetic postmenopausal women using metformin had collagen abundance organization were similar premenopausal ovaries from nondiabetic women. In this study, we investigated effects on mouse at single-cell level. discovered treatment prevented...

In an effort to identify novel endo-alpha-N-acetylgalactosaminidases (endo-alpha-GalNAcases), four potential genes were cloned. Three of the expressed proteins EngEF from Enterococcus faecalis, EngPA Propionibacterium acnes, and EngCP Clostridium perfringens purified characterized. Their substrate specificity was investigated compared commercially available endo-alpha-GalNAcases Streptococcus pneumoniae (EngSP) Alcaligenes sp. (EngAL). All enzymes incubated with various synthetic substrates,...

// David A. Landry 1 , Het T. Vaishnav and Barbara C. Vanderhyden Cancer Therapeutics Program, Ottawa Hospital Research Institute Department of Cellular Molecular Medicine, University Ottawa, Canada Correspondence to: Vanderhyden, email: bvanderhyden@ohri.ca Keywords: ovarian fibrosis; cancer; female reproduction; fertility; metformin Received: October 30, 2020 Accepted: November 03, Published: 24, Copyright: © et al. This is an open access article distributed under the terms Creative...

The disposition of an aqueous suspension procaine penicillin G (300 000 U/ mL) was studied in feedlot steers. Four groups three steers were used. Steers 1 and 2 received once daily for 5 days intramuscularly (i.m.) at a dose 24 U/kg (group 1) or 66 2). injection on the last day administered gluteal muscle. group 3 (i.m. neck injection) 4 [subcutaneous (s.c.) injection] each single U/kg. From every animal, after following 4, series blood samples taken fixed time intervals. plasma from these...

Abstract Mammalian folliculogenesis is a complex process that involves the regulation of chromatin structure for gene expression and oocyte meiotic resumption. The SWI/SNF remodeler using either Brahma-regulated 1 (BRG1) or BRM (encoded by Smarca4 Smarca2, respectively) as its catalytic subunit. SMARCA4 loss associated with rare type ovarian cancer; however, function during remains poorly understood. In this study, we describe phenotype BRG1 mutant mice to better understand role in female...

Procaine penicillin G was administered by intramuscular (IM) injection to groups of healthy 100 kg market pigs at the approved label dose (15000 IU/kg body weight), once daily for three consecutive days; or an extra‐label (66000 five days. Penicillin residue depletion followed in plasma, tissue and sites using a liquid chromatographic method. Groups were killed 1, 2, 3, 4, 5 8 days after last with dose. not detected liver 1 day withdrawal, muscle fat 2 plasma 4 skin kidney withdrawal. Other...

To explore whether resistin, an adipose derived hormone linked to insulin resistance, influence steroidogenic genes expressions and Leydig cells function or not. Various cell lines were exposed increasing doses of resistin with without cAMP. Changes monitored at the protein level for signal transducer activator transcription (STAT) factors components, acute regulatory (STAR) cholesterol side-chain cleavage enzyme (CYP11A1), progesterone production viability. Resistin had no effect on...

Summary Plasma concentration of penicillin G was evaluated in beef steers after administration either a combination benzathine and procaine 1:1 mixture at dosage 9,000 U/kg body weight, im (n = 5), 24,000 U/kg, or 8,800 sc alone 12,000 7). measured by use high-performance liquid chromatography assay that had limit determination 0.005 µg/ml. At for this , which are approved label recommendations Canada, the United States, respectively, mean (± sem ) peak plasma 0.58 0.15) 0.44 0.02) µg/ml,...

Abstract The ovarian surface epithelium (OSE) is a monolayer of epithelial cells surrounding the ovary that ruptures during each ovulation to allow release oocyte. This wound quickly repaired, but mechanisms promoting repair are poorly understood. contribution tissue-resident stem in homeostasis several tissues widely accepted, their involvement OSE unclear. We show traits associated with can be increased following exposure cytokine TGFB1, overexpression transcription factor Snai1 , or...

Abstract The contribution of benzathine penicillin G to residues in tissues and injection sites yearling beef steers was assessed by treating seven groups five with either procaine together or alone. Steers were injected a commercial combination according the Canadian (intramuscular) United States (subcutaneous) label dosages 8600 8800 IU G/kg body weight, respectively. They killed 14 30 days after intramuscular injections, subcutaneous injections. At withdrawal times, days, levels for all...

The implications of the epithelial–mesenchymal transition (EMT) mechanisms in initiation and progression epithelial ovarian cancer (EOC) remain poorly understood. We have previously shown that suppression antigen receptor LY75 directs mesenchymal–epithelial (MET) EOC cell lines with mesenchymal phenotype, associated loss Wnt/β-catenin signaling activity. In present study, we used LY75-mediated modulation EMT cells as a model order to investigate vivo specific role cells, an (E), (M) or mixed...

The aim of the clinician when using antimicrobials is to provide effective treatment while minimizing residues in milk and meat. Among all antibiotics used food animals,procaine penicillin G (PPG) one oldest still most widely used. Over years, recommended dosage has increased from 7, 500 IU /kg I. M. (the label dosage) recommended, but not approved 11,000 22,000 IU/kg administered I.M. or s.c. (1). subcutaneous route easier use large volumes need be administered.
 Because discrepancy...

The objective of this study was to determine the highest dose procaine penicillin G (PPG) which could be administered allow a withdrawal period 96 hours in milk milking dairy cows. Using two 4x4 latin square designs, groups lactating cows (n=4 per group) received 4 different doses (7,000, 14,000, 21,000 and 28,000 IU/kg 20 mL/site 300 000 IU/mL PPG . Each once daily for 5 consecutive days each cow by either intramuscular (square 1) or subcutaneous route 2). new every 3 weeks. Milk samples...