Hepatocellular carcinoma (HCC) is a serious and potentially fatal form of cancer associated with liver damage. New anticancer drugs are increasingly needed due to the increasing number cases every year. In this study, diarylheptanoids (DAH) from Alpinia officinarum were examined for their antitumor activity against DAB-induced HCC in mice, as well ability reduce Assays cytotoxicity conducted using MTT. The Swiss albino male mice given DAH sorafenib (SOR) either single treatments or...

Brown algae earned importance by virtue of their promising secondary metabolites reasonable biological activities. Herein, the antioxidant, antimicrobial, and anticancer effects crude extracts obtained from two Egyptian brown seaweeds, Sargassum linearifolium Cystoseira crinita were evaluated. Phytochemical GC-MS analyses revealed numerous active in C. cold methanolic extract (CCME) S. hot aqueous (SHAE). Both SHAE CCME exhibited comparable DPPH (124.5 vs 125.6 µg/ml) ABTS (257.1 254.8...

Abstract Background Natural products from plants are very safe as compared to synthetic ones, so the aim of this study was assess in vitro antioxidant and antitumor activities ethanolic extracts four Egyptian wild plant species ( Varthemia candicans , Peganum harmala Suaeda vermiculata Conyza dioscoridis ), well polyphenols flavonoid contents with gas chromatography–mass spectrometry (GC-MS). The activity assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) determine 50% inhibition DPPH...

Breast cancer cells abnormally express vasopressin (AVP) and its receptors. The effect of AVP is largely orchestrated through downstream signaling by receptor-mediated endocytosis (RME), in which Dynamin 2 (Dyn2) plays an integral role vesicle closure. In this work, luminal A breast were treated with AVP, then Dynasore (DYN) was employed to inhibit Dyn2 explore the combined inhibition on survival cells. results revealed that DYN alone demonstrated a concentration-dependent cytotoxic...

Numerous reports have explored the roles of different genetic variants in miRNA biogenesis mechanisms and progression various types carcinomas. The goal this study is to explore association between XPO5*rs34324334 RAN*rs14035 gene susceptibility hepatocellular carcinoma (HCC). In a cohort 234 participants (107 HCC patients 127 unrelated cancer-free controls) from same geographic region, we characterized allelic discrimination using PCR-RFLP performed subgroup analysis multivariate...

Background and Objective: Although the anticancer potentials of water-insoluble drugs are improved by nanoformulation, other intervening factors may contribute in drug efficacy. This work was designated to explore effect paclitaxel-loaded Poly(Lactic-co-Glycolic Acid) (PLGA) nanoparticles on viability cancer cells, expression Taxol Resistance gene I (TXR1) paclitaxel metabolizing genes. Methods: Paclitaxel loaded PLGA Nanoparticles (PTX-NPs) were prepared, physically characterized used...

Several studies addressed the contribution of fat mass and obesity-associated (FTO) leptin receptor (LEPR) polymorphisms for susceptibility to obesity among different ethnic subjects. The main purpose this work is evaluate association these polymorph\isms with Egyptian subjects.This case-control study was carried out on 110 unrelated obese subjects who were compared 122 controls. Their genomic DNA genotyped using PCR technique.The allelic frequencies FTO rs9939609 (A) LEPR rs1137101 (223R)...

This study aims to investigate the interactions between osthole extracted from Egyptian citrus fruits as HDACs inhibitor by theoretical and practically. Besides, was assed anti-cancer activity. In this study, fruit characterized. The role of in vitro estimated evaluated antitumor activity against human lung cancer cells (A549), Caspase-9 detected. results obtained GC-MS indicate that grapefruit showed highest concentration compared other fruits. Moreover, competitively inhibits HDACs....

Introduction: Plant-mediated synthesis of silver nanoparticles (AgNPs) is accounted as an ecofriendly process. The present study was conducted to estimate the potency biogenic AgNPs against Ehrlich ascites carcinoma (EAC) cells in vitro and EAC-bearing mice vivo.
 Methods: were prepared using mango leaves extract characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), transmission microscopy (TEM). mouse model established intraperitoneal injection 1 x 106 EAC...

This work was designated to explore the effect of glucocorticoid (dexamethasone) on responsiveness hepatoma cells Paclitaxel (PTX) and expression Taxol resistance gene (Txr1) paclitaxel metabolizing genes.Hepatocellular carcinoma (HepG2) were treated with standard or solvent containing (Taxol) in presence absence dexamethasone (DEX).Cell viability apoptosis determined by MTT assay flow cytometry, respectively.Also, total RNA isolated, reverse transcribed used determine levels Txr1, CYP 3A4,...

KLF6 is ubiquitously expressed in human tissues and regulates many pathways such as differentiation, development, cellular proliferation, growth-related signal transduction, apoptosis. We previously demonstrated that expression altered during liver carcinogenesis. More importantly, invalidation results cell cycle progression inhibition apoptosis of cancer cells. On the other hand, enforced variant 2 (SV2) induces death by Thus, we others its splicing variants play a critical role cancer....

Quinazolinone derivatives have been studied as both in vitro and vivo inhibitors of aspartate transcarbamylase (ATCase). In treatment mammalian ATCase with four compounds revealed that they inhibited enzyme activity 2-phenyl-1,3-4(H)benzothiazin-4-thione was the most potent one. This compound acts a noncompetitive inhibitor towards carbamoyl phosphate. The values inhibition constant (Ki) indicate this exerts inhibitory effect upon activity. Moreover, different doses these showed also an...

Chemotherapy is currently the most utilized treatment for cancer. Therapeutic potential of metal complexes in cancer therapy has attracted a lot interest. The mechanisms action organometallic are poorly understood.This study was designed to explore governing anti-proliferative effect free ligand N1,N6-bis((2-hydroxynaphthalin-1-yl)methinyl)) adipohydrazone (H2L) and its Mn(II), Co(II), Ni(II) Cu(II).Cells were exposed H2L or where cell viability determined by MTT assay. Cell cycle analysed...

The anti-inflammatory roles of Mesenchymal stem cells (MSCs) and glucocorticoids are well-reported in both preclinical clinical studies. However, it is not clear how far MSC-secretome offers sufficient protection against acute liver failure (ALF) compared to glucocorticoids. To answer this query, was induced mice by a single toxic dose (400 mg/kg) acetaminophen (APAP). Then were treated with Dexamethasone or transfused MSC-secretome, which derived from DEX-treated bone marrow mesenchymal...

Gold nanoparticles (AuNPs) attracted great attention due to their unusual and fascinating properties, applications in many fields. The synthesis of AuNPs was mediated by Spirulina platensis which observed the color change from yellow red detected using UV-visible spectra that showed a strong absorption peak at 400 nm indicating presence AuNPs. Characterization performed through different techniques such as Fourier transform infrared spectroscopy (FTIR) spectral analysis shows an array...

The relationship of leptin (LEP) and polymorphism receptor (LEPR) were studied in patients with hepatocellular carcinoma (HCC) compared those liver cirrhosis to find out the extent risk LEPR on HCC.Serum LEP level Q223R gene determined 300 disease categorized equally into five groups' healthy volunteers, hepatitis C (HCV), non-alcoholic steatohepatitis (NASH), HCC. was amplified by polymerase chain reaction (PCR) then digested MSP1 restriction enzyme.The isolated 212 bp sequenced. serum...

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Elmetwalli et al., 2019). HCC is the fourth and second leading cause of cancer mortality in men women, respectively (Zhu 2016).Antioxidant, antiproliferative, anti-invasive, apoptotic, antimutagenic, anticarcinogenic, antitumor, cytotoxic activity, are all strategies to suppress liver (Roleira 2015).Diarylheptanoids Cytotoxicity Anti-Carcinogenic Activity:

Abstract Purpose: Breast cancer cells abnormally express arginine vasopressin hormone (AVP) and its receptors. Clathrin-mediated endocytosis (CME) involves a machinery of intracellular endocytic proteins, including large Dynamins. The impact Dynamin inhibition on the survivability metastasis triple-negative breast cells, stimulated by AVP, was not well addressed. Methods: To explore this, 2 selectively inhibited dynasore (DYN) in MDA MB-231 which prestimulated with or cotreated with,...

Among seven homologs of cytochrome b561 in a model organism C. elegans, Cecytb-2 was confirmed to be expressed digestive organs and considered as homolog human Dcytb functioning ferric reductase. protein Pichia pastoris cells, purified, reconstituted into phospholipid bilayer nanodisc. The nanodisc environments extremely stable more reducible with ascorbate than detergent-micelle state. We the reductase activity by analyzing oxidation ferrous heme upon addition substrate under anaerobic...

Abstract Breast cancer cells abnormally express vasopressin (AVP) and its receptors. The effect of AVP is largely orchestrated through downstream signaling by receptor mediated endocytosis (RME), in which Dynamin 2 (Dyn2) plays an integral role vesicle closure. In this work, luminal A breast were treated with AVP, then Dynasore (DYN) was employed to inhibit Dyn2 explore the combined inhibition on survival cells. results revealed that DYN alone demonstrated a concentration dependent cytotoxic...