A5023083244- Marine Invertebrate Physiology and Ecology
- Lipid Membrane Structure and Behavior
- Ferroptosis and cancer prognosis
- Marine Toxins and Detection Methods
- Erythrocyte Function and Pathophysiology
- Marine Sponges and Natural Products
- Protist diversity and phylogeny
- Nanopore and Nanochannel Transport Studies
- Heme Oxygenase-1 and Carbon Monoxide
- RNA modifications and cancer
- Trace Elements in Health
- Marine and environmental studies
- Mitochondrial Function and Pathology
- Inflammasome and immune disorders
- RNA Interference and Gene Delivery
- Caveolin-1 and cellular processes
- Antimicrobial Peptides and Activities
- interferon and immune responses
- Cancer, Lipids, and Metabolism
- Streptococcal Infections and Treatments
- Hemoglobin structure and function
- Cell death mechanisms and regulation
- Bacteriophages and microbial interactions
- Electrochemical Analysis and Applications
- Phagocytosis and Immune Regulation
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2019-2025
University of Cologne
2020-2025
University of Havana
2011-2023
University of Tübingen
2019
Cuban Neuroscience Center
2011
Ferroptosis is an iron-dependent form of regulated necrosis associated with lipid peroxidation. Despite its key role in the inflammatory outcome ferroptosis, little known about molecular events leading to disruption plasma membrane during this type cell death. Here we show that a sustained increase cytosolic Ca2+ hallmark ferroptosis precedes complete bursting cell. We report damage nanopores few nanometers radius and but not peroxidation, can be delayed by osmoprotectants. Importantly,...
Abstract Constitutive mitochondrial dynamics ensure quality control and metabolic fitness of cells, their dysregulation has been implicated in various human diseases. The large GTPase Dynamin-related protein 1 (Drp1) is intimately involved mediating constitutive fission cell death pathways. During ferroptosis, a recently identified type regulated necrosis driven by excessive lipid peroxidation, fragmentation observed. Yet, how this whether it ferroptotic remained unexplored. Here, we provide...
Sticholysin I (St I) is a pore-forming toxin (PFT) produced by the Caribbean Sea anemone Stichodactyla helianthus belonging to actinoporin protein family, unique class of eukaryotic PFT. As for actinoporins, it has been proposed that presence cholesterol (Chol) and coexistence lipid phases increase binding target membrane ability. However, little known about role structure dynamics (phase state, fluidity, domains) on activity actinoporins or which regions are most favorable insertion,...
Crystallographic data of the dimeric and octameric forms fragaceatoxin C (FraC) suggested key role a small hydrophobic protein-protein interaction surface for actinoporins oligomerization pore formation in membranes. However, site-directed mutagenesis studies supporting this hypothesis others are still lacking. Here, we demonstrate that disrupting between V60 F163 (FraC numbering scheme) interface FraC, equinatoxin II (EqtII), sticholysin (StII) impairs activity these proteins. Our results...
Ferroptosis is a form of regulated necrosis characterized by the iron-dependent accumulation lipid peroxides in cell membranes. However, how oxidation via iron-mediated Fenton reactions affects biophysical properties cellular membranes and these changes contribute to opening plasma membrane pores are major questions field. Here, we dynamics alterations during induced onsite chemically defined vitro model systems. We find that vesicle permeabilization kinetically correlates with appearance...
Summary Ferroptosis is an iron-dependent form of regulated necrosis associated with lipid peroxidation. Despite its key role in the inflammatory outcome ferroptosis, little known about molecular events leading to disruption plasma membrane during this type cell death. Here we show that a sustained increase cytosolic Ca 2+ hallmark ferroptosis precedes complete bursting cell. We report damage nanopores few nanometers radius and but not peroxidation, can be delayed by osmoprotectants....
<title>Abstract</title> Constitutive mitochondrial dynamics ensure quality control and metabolic fitness of cells, their dysregulation has been implicated in various human diseases. The large GTPase Dynamin-related protein 1 (Drp1) is intimately involved mediating constitutive fission cell death pathways. During ferroptosis, a recently identified type regulated necrosis driven by excessive lipid peroxidation, fragmentation observed. Yet, how this whether it ferroptotic remained unexplored....
Actinoporins have emerged as archetypal α-pore-forming toxins (PFTs) that promote the formation of pores in membranes upon oligomerization and insertion an α-helix pore-forming domain bilayer. These proteins been used active components immunotoxins, therefore, understanding their lytic mechanism is crucial for developing this other applications. However, how biophysical properties membrane modulate generated by actinoporins remains unclear. Here we studied effect fluidity on permeabilizing...
Abstract Ferroptosis is a form of regulated necrosis characterized by the iron-dependent accumulation lipid peroxides in cell membranes. However, how oxidation affects biophysical properties cellular membranes and these changes contribute to opening membrane pores are major questions field. Therefore, understanding interplay between oxidized phospholipids alterations essential unravel underlying mechanisms ferroptosis. Here, we upon vitro model systems using vesicles supported bilayers...