A5068151631- melanin and skin pigmentation
- Enzyme Catalysis and Immobilization
- Biotin and Related Studies
- Biochemical Analysis and Sensing Techniques
- Microbial Fuel Cells and Bioremediation
- Photosynthetic Processes and Mechanisms
- Polyamine Metabolism and Applications
- Biofuel production and bioconversion
- Antimicrobial agents and applications
- Biochemical and biochemical processes
- Advanced Biosensing Techniques and Applications
- Microbial Metabolism and Applications
- Enzyme-mediated dye degradation
- Microfluidic and Capillary Electrophoresis Applications
- Polymer Surface Interaction Studies
- Enzyme Production and Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemistry and Chemical Engineering
- Surface Modification and Superhydrophobicity
- Iron oxide chemistry and applications
- Bioeconomy and Sustainability Development
- Analytical Chemistry and Sensors
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Nanofabrication and Lithography Techniques
University of St. Gallen
2015-2024
Swiss Federal Laboratories for Materials Science and Technology
2012-2020
VTT Technical Research Centre of Finland
2009-2017
BioSurfaces (United States)
2016
Tieto (Finland)
2011
Controlled and efficient immobilization of specific biomolecules is a key technology to introduce new, favorable functions materials suitable for biomedical applications. Here, we describe an innovative efficient, two-step methodology the stable various biomolecules, including small peptides enzymes onto TEMPO oxidized nanofibrillated cellulose (TO-NFC). The introduction carboxylate groups NFC by oxidation provided high surface density negative charges able drive adsorption take part in...
Tracing heavy metals is a crucial issue in both environmental and medical samples. In this work, sensing biomolecule, the cyanobacterial C‐phycocyanin (CPC), integrated into nanocellulose matrix, with this, biosensor for copper ions developed. The assembly of CPC‐functionalized red‐fluorescent, copper‐sensitive hybrid film “CySense”, enhances protein stability facilitates reuse regeneration sensor several cycles over 7 days. CySense suitable analysis complex samples such as human serum...
Abstract Progressive antibiotic resistance is a serious condition adding to the challenges associated with skin wound treatment, and antibacterial dressings alternatives antibiotics are urgently needed. Cellulose‐based membranes increasingly considered as dressings, necessitating further functionalization steps. A bifunctional peptide, combining an antimicrobial peptide (AMP) cellulose binding (CBP), designed. AMPs affect bacteria via multiple modes of action, thereby reducing evolutionary...
Resistance to antibiotics has posed a high demand for novel strategies fight bacterial infections. Antimicrobial peptides (AMPs) are promising alternative conventional antibiotics. However, their poor solubility in water and sensitivity degradation limited application. Here, we report the design of smart, pH-responsive antimicrobial nanobiocomposite material based on AMP nisin 2,2,6,6-tetramethyl-1-piperidinyloxyl-oxidized nanofibrillated cellulose (TONFC). Morphological transformations...
The direct and specific detection of biomarkers activity is crucial as it can allow monitoring the state tissue or wound, well progression inflammatory process.
Enzymatic crosslinking of proteins is often limited by the steric availability target residues, as tyrosyl side chains in case tyrosinase. Carrying an N-terminal peptide-tag containing two tyrosine fluorescent protein C-phycocyanin HisCPC from Synechocystis sp. PCC6803 was crosslinked to high-molecular weight forms with Crosslinking tyrosinase presence L-tyrosine produced non products. Incubated tyrosinase, could also be immobilized amino-modified polystyrene beads thus conferring a blue...
The use of enzymes in materials science allows the development unique and innovative functional materials. As biomolecules, can provide exceptional features such as substrate specificity, rate acceleration, regio-, chemo-, stereoselectivity within catalyzed reactions. Many are suitable for biomaterials modification because their natural origin or they catalyze a desired reaction between non-natural substrates. In native form, after tailoring by genetic engineering, complement traditional...
This protocol describes an in vitro fluorogenic assay to measure the proteolytic activity and identify inhibitors of Mpro, main protease produced by SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2). Studies potential Mpro mainly rely on silico molecular dynamics simulations or FRET (Fluorescence Resonance Energy Transfer) substrates. The is based aminomethyl coumarin substrate. High sensitivity, specificity, easily detectable fluorescent read-out are advantages offered this rapid...
Laccases and laccase-like multi-copper oxidases (LMCOs) are versatile robust biocatalysts applied in a variety of oxidative processes, various studies have attempted to improve their catalytic activity. Here we report the engineering bacterial LMCO for enhanced oxidation lignin-related compound guaiacol by combination structure-guided mutagenesis DNA shuffling. Mutant L9 showed 1.39 mM Km 2.5-fold increase turnover rate (kcat/Km = 2.85·104 M-1s-1).
Abstract Background Cholesterol oxidases are important enzymes for applications such as the analysis of cholesterol in clinical samples, synthesis steroid derived drugs, and considered potential antibacterial drug targets. Results The gene choA encoding a oxidase from Chryseobacterium gleum DSM 16776 was cloned into pQE-30 expression vector heterologously expressed Escherichia coli JM109 co-transformed with pRARE2. N-terminally His-tagged (CgChoA) assigned to be monomer solution by size...
Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization protein biosensor in nanoparticle was achieved through specific interaction between hexa-histidine tag a calcium-silicate complex silica matrix. Encapsulation preserved affinity respective sugar. Compared to free biosensors, encapsulation had stabilizing effect on towards chemical thermal denaturation. The demonstrated immobilization strategy sensing proteins...