A5016162911- biodegradable polymer synthesis and properties
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Microplastics and Plastic Pollution
- Antimicrobial Peptides and Activities
- Enzyme Catalysis and Immobilization
- Electrospun Nanofibers in Biomedical Applications
- Carbon dioxide utilization in catalysis
- Biopolymer Synthesis and Applications
- Antibiotic Resistance in Bacteria
- Polymer Surface Interaction Studies
- Wound Healing and Treatments
- Fungal and yeast genetics research
- Nanoparticles: synthesis and applications
- Dental materials and restorations
- Kidney Stones and Urolithiasis Treatments
- Tardigrade Biology and Ecology
- Computational Drug Discovery Methods
- Biosensors and Analytical Detection
- Bacterial Identification and Susceptibility Testing
- Polydiacetylene-based materials and applications
- Urinary Tract Infections Management
- Parkinson's Disease Mechanisms and Treatments
- Graphene and Nanomaterials Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
Swiss Federal Laboratories for Materials Science and Technology
2016-2025
Nanchang University
2023-2025
First Affiliated Hospital of Jiangxi Medical College
2024-2025
University of Wyoming
2003-2024
First Affiliated Hospital of Nanchang University
2023-2024
Xi'an University of Architecture and Technology
2024
Nanjing University of Chinese Medicine
2023-2024
Shanghai Institute of Materia Medica
2023-2024
Chinese Academy of Sciences
2023-2024
China Iron and Steel Research Institute Group
2024
The 1,852,442-bp sequence of an M1 strain Streptococcus pyogenes , a Gram-positive pathogen, has been determined and contains 1,752 predicted protein-encoding genes. Approximately one-third these genes have no identifiable function, with the remainder falling into previously characterized categories known microbial function. Consistent observation that S. is responsible for wider variety human disease than any other bacterial species, more 40 putative virulence-associated identified....
Viability staining with SYTO9 and propidium iodide (PI) is a frequently used tool in microbiological studies. However, data generated by such routinely method are often not critically evaluated for their accuracy. In this study we aim to investigate the critical aspects of using Staphylococcus aureus Pseudomonas aeruginosa as model microorganisms high throughput studies microtiter plates. or PI was added alone consecutively together cells fluorescence intensities were measured microplate...
Ultralight and highly flexible biopolymer aerogels, composed of biomimetic cellular microstructures formed from cellulose nanofibers silver nanowires, are assembled via a convenient facile freeze-casting method. The lamellar, honeycomb-like, random porous scaffolds successfully achieved by adjusting freezing approaches to modulate the relationships between macroscopic mechanical electromagnetic interference (EMI) shielding performances. Combining transformation arising in situ compression...
With the increasing concern over environmental impact of conventional chemical methods, environmentally friendly processes, commonly known as green chemistry, for synthesis nanoparticles have gained growing interest in field nanobiotechnology. This review focuses on metallic (NPs) based chemistry and their applications new drug delivery system anticancer antimicrobial treatment. The encompasses a survey production characterization synthetic NPs, along with an examination physico-chemical...
Intracellular bacteria in latent or dormant states tolerate high-dose antibiotics. Fighting against these opportunistic has been a long-standing challenge. Herein, the design of cascade-targeting drug delivery system (DDS) that can sequentially target macrophages and intracellular bacteria, exhibiting on-site delivery, is reported. The DDS fabricated by encapsulating rifampicin (Rif) into mannose-decorated poly(α-N-acryloyl-phenylalanine)-block-poly(β-N-acryloyl-d-aminoalanine)...
We investigated the ability of GM1 to induce phosphorylation tyrosine kinase receptor for neurotrophins, Trk, in rat brain, and activation possible down-stream signaling cascades. increased phosphorylated Trk (pTrk) slices striatum, hippocampus frontal cortex a concentration- time-dependent manner, enhanced activity resulting autophosphorylation. The pTrk was shared by other gangliosides, blocked selective inhibitors K252a AG879. induced TrkA > TrkC TrkB region-specific distribution. Adding...
This paper presents evidence that a member of the L1 family ankyrin-binding cell adhesion molecules is substrate for protein tyrosine kinase(s) and phosphatase(s), identifies highly conserved FIGQY in cytoplasmic domain as principal site phosphorylation, demonstrates phosphorylation abolishes activity. Neurofascin expressed neuroblastoma cells subject to after activation kinases by NGF or bFGF inactivation phosphatases with vanadate dephostatin. Furthermore, both neurofascin related molecule...
Due to the increased emergence of antimicrobial resistance, alternatives minimize usage antibiotics become attractive solutions. Biophysical manipulation material surface topography prevent bacterial adhesion is one promising approach. To this end, it essential understand relationship between topographical features and bactericidal properties in order develop antibacterial surfaces.In work a systematic study effects on activity nanostructured surfaces presented. Nanostructured Ormostamp...
Various methods have been reported to quantify total biofilm or different components of biofilm; however, these are often confusedly used, leading discrepancies and misleading results. In this study, for quantification biofilm, including those biomass, amount bacterial cells, viable cell number, extracellular polymeric substances, were systematically compared in microtiter plates. To evaluate which method is suitable assessment removal killing, samples treated with various cleaners...
Hospital-acquired infections can cause serious complications and are a severe problem because of the increased emergence antibiotic-resistant bacteria. Biophysical modification material surfaces to prevent or reduce bacteria adhesion is an attractive alternative antibiotic treatment. Since stainless steel widely used for implants in hospital settings, this work, we investigate effect surface topographies on bacterial early biofilm formation. Stainless samples with different roughnesses Rq...
We present here a technological platform for engineering Au nanotopographies by templated electrodeposition on antibacterial surfaces.
With the increased occurrence of antibiotic-resistant bacteria, alternatives to classical antibiotics are urgently needed for treatment various infectious diseases. Medicinal plant extracts among promising candidates due their bioactive components. The aim this study was prepare niosome-encapsulated Echinacea angustifolia extract and its efficacy against multidrug-resistant Klebsiella pneumoniae strains. Encapsulation first optimized by Design Experiments, followed empirical study. obtained...
Chronic wounds are not only a burden for patients but also challenging clinic treatment due to biofilm formation. Here, we utilized the phenomenon that chronic possess an elevated local pH of 8.9 and developed pH-sensitive silica nanoparticles (SiNPs) achieve targeted drug release on alkaline optimized utility. Chlorhexidine (CHX), disinfectant antiseptic, was loaded into SiNPs as model drug. The CHX displayed 4 - 5 fold higher at 8.0 8.5 than 6.5, 7.0 7.4. CHX-SiNPs furthermore exhibited...
Abstract Reusable face masks are an important alternative for minimizing costs of disposable and surgical during pandemics. Often complementary to washing, a prolonged lifetime relies on the incorporation self-cleaning materials. The development mask materials requires presence durable catalyst deactivate contaminants microbes after long-term use without reducing filtration efficiency. Herein, we generate fibers by functionalizing silicone-based (polydimethylsiloxane, PDMS) fibrous membranes...
The rising threat from infectious pathogens poses an ever-growing challenge. Metal-based nanomaterials have gained a great deal of attention as active components in antimicrobial coatings. Here, we report on the development readily deployable, sprayable surface coatings for high-touch stainless steel surfaces that are ubiquitous many healthcare facilities to combat spread pathogens. We synthesized mesoporous silica nanoparticles (MSNs) with different functional groups, namely, amine...
Abstract Background Pseudomonas putida KT2440 is able to synthesize large amounts of medium-chain-length polyhydroxyalkanoates (mcl-PHAs). To reduce the substrate cost, which represents nearly 50% total PHA production xylose, a hemicellulose derivate, was tested as growth carbon source in an engineered P. strain. Results The genes encoding xylose isomerase (XylA) and xylulokinase (XylB) from Escherichia coli W3110 were introduced into KT2440. recombinant exhibited XylA activity 1.47 U XylB...
The regulation of medium-chain-length polyhydroxyalkanoates (mcl-PHA) metabolism in Pseudomonas putida GPo1 was studied by analysis enzymes bound to PHA granules and involved fatty acid oxidation. N-terminal sequencing granule-bound revealed the presence polymerase (PhaC) depolymerase (PhaZ) an acyl-CoA synthetase (ACS1), which recently found be associated with vivo study. acs1 knockout mutant accumulated 30-50% less than its parental strain, confirming involvement ACS1 metabolism. Isolated...
Nephrons comprise a blood filter and an epithelial tubule that is subdivided into proximal distal segments, but what directs this patterning during kidney organogenesis not well understood. Using zebrafish, we found the HNF1β paralogues hnf1ba hnf1bb, which encode homeodomain transcription factors, are essential for normal segmentation of nephrons. Embryos deficient in hnf1bb did express segment markers, yet still developed tubule. Initiating hnf1ba/b expression required Pax2a Pax8,...
Stimuli-responsive nanocarriers based on lipid self-assemblies have the potential to provide targeted delivery of antimicrobial peptides, limiting their side effects while protecting them from degradation in biological environments. In present study, we design and characterize a simple pH-responsive nanomaterial, formed through self-assembly oleic acid (OA) with human cathelicidin LL-37 as model for an amphiphilic peptide. Colloidal transformations core–shell cylindrical micelles...
Significance We tested the hypothesis that a form of mitochondrial dysfunction alters homeostasis cytosolic Parkinson disease (PD)-associated protein α-synuclein (α-syn). Using yeast and worm models PD, we show low levels phosphatidylethanolamine (PE), caused by depletion phosphatidylserine decarboxylase (psd), lead to decreased respiration, endoplasmic reticulum (ER) stress, high α-syn cytoplasmic foci, slow growth. Ethanolamine, which replenishes PE through Kennedy pathway, diminished ER...