A5047344586- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Erythrocyte Function and Pathophysiology
- Micro and Nano Robotics
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Connexins and lens biology
- Protein Structure and Dynamics
- Microfluidic and Bio-sensing Technologies
- Analytical Chemistry and Sensors
- Bacterial Genetics and Biotechnology
- Neurobiology and Insect Physiology Research
- Protist diversity and phylogeny
- Heat shock proteins research
- Analytical chemistry methods development
- Advanced Chemical Sensor Technologies
- Orbital Angular Momentum in Optics
- Hemoglobin structure and function
- Electrochemical Analysis and Applications
- Circadian rhythm and melatonin
- Magnetic and Electromagnetic Effects
- Ion Channels and Receptors
- Physical Activity and Health
- Thermography and Photoacoustic Techniques
- Neural dynamics and brain function
Shibaura Institute of Technology
2015-2024
ORCID
2021
Kyoto University
1986-2017
Kyoto University Hospital
2017
Japan Science and Technology Agency
2004-2016
University of Tsukuba
2003-2015
University of Maryland, College Park
2010-2015
Okazaki Institute for Integrative Bioscience
2006-2008
Bio-oriented Technology Research Advancement Institution
2007
National Institutes of Natural Sciences
2006-2007
Abstract Mechanosensitive (MS) ion channels respond to mechanical stress and convert it into intracellular electric ionic signals. Five MS channel families have been identified in plants, including the Mid1-Complementing Activity (MCA) channel; however, its activation mechanisms not elucidated detail. We herein demonstrate that MCA2 is a Ca 2+ -permeable directly activated by membrane tension. The N-terminal 173 residues of MCA1 were synthesized vitro, purified, reconstituted artificial...
MscS is a mechanosensitive channel that ubiquitous among bacteria. Recent progress in the genome projects has revealed homologs of are also present eukaryotes, but whether they operate as ion channels unknown. In this study we cloned MSC1, homolog Chlamydomonas, and examined its function when expressed Escherichia coli. Full-length MSC1 was not functional E. coli cells. However, removal N-terminal signal sequence (DeltaN-MSC1) reversed effect. DeltaN-MSC1 found to open response membrane...
SEC66 encodes the 31.5-kDa glycoprotein of Sec63p complex, an integral endoplasmic reticulum membrane protein complex required for translocation presecretory proteins in Saccharomyces cerevisiae. DNA sequence analysis predicts a 23-kDa with no obvious NH2-terminal signal but one domain sufficient length and hydrophobicity to span lipid bilayer. Antibodies directed against recombinant form Sec66p were used confirm location that is 31.5 kDa. A null mutation renders yeast cells temperature...
In the mechanism underlying phototactic behavior of Chlamydomonas, Ca(2+) has been thought to control dominance between two flagella so as steer cell correct directions. A newly isolated mutant, lsp1, that displays weak phototaxis was found be defective in this Ca(2+)-dependent shift flagellar dominance; demembranated and reactivated models, trans flagellum (the farthest from eyespot) beat more strongly than other cis flagellum) about half cells regardless concentration <10(-9) M 10(-6) M, a...
Cells actively regulate the macromolecular excluded volume of cytoplasm to maintain reciprocal fraction free aqueous solution that is optimal for intracellular processes. However, mechanisms whereby cells sense this critical parameter remain unclear. The mechanosensitive channel small conductance (MscS channel), which major regulator turgor in bacteria, mediates efflux osmolytes response increased membrane tension. At moderate sustained tensions produced by a decrease external osmolarity,...
Bacterial cells avoid lysis in response to hypoosmotic shock through the opening of mechanosensitive channel MscL. Upon opening, MscL is thought expand plane membrane and form a large pore with an estimated diameter 3-4 nm. Here, we set out analyze closed open structure cell-free To this end, characterized function wild-type mutant protein (G22N MscL) that spontaneously adopts substate. Patch-clamp analysis had been reconstituted into liposomes revealed was activated only by mechanical...
The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion Corynebacterium glutamicum. Patch clamp experiments revealed that had a significantly smaller gating threshold than wild-type counterpart and displayed strong hysteresis, suggesting gain-of-function of leads to oversecretion glutamate.
Deposits on soft contact lenses of high water content were investigated morphologically and chemically compared with those conventional poly (2-hydroxyethyl methacrylate). The material the examined in this investigation was crosslinked copolymer methyl methacrylate N-vinylpyrrolidone a higher than 70%. Morphologically, deposits found to have no characteristics distinguishable from lenses. By electron microscopic observation cross section lens that had become opaque, it confirmed deposit...