A5055507709- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Growth Hormone and Insulin-like Growth Factors
- Reproductive Physiology in Livestock
- Genomic variations and chromosomal abnormalities
- Nuclear Receptors and Signaling
- Endoplasmic Reticulum Stress and Disease
- Genetic and phenotypic traits in livestock
- Adipose Tissue and Metabolism
Northwest A&F University
2019-2021
University of Nyala
2020-2021
Circular RNAs (circRNAs) are ubiquitous endogenous RNA found in various organisms that can regulate gene expression eukaryotes. However, little is known about potential roles for circRNAs muscle development. We analyzed circRNA sequencing data of bovine skeletal tissue and differential circTitin (circTTN) fetal adult tissue. then further studied the role circTTN Overexpression inhibition together elicited its promoting proliferation differentiation primary myoblasts. Mechanistically, showed...
MicroRNAs are small, single stranded, and noncoding RNAs that have been proven to be potent regulators of adipogenesis. However, the role bta-miR-149-5p in regulating bovine adipogenesis is still unclear. Expression profiling different stages revealed was enriched proliferation stage, also on Day 9 differentiation adipocytes. Our gain function study showed can negatively regulate both adipocyte differentiation. Overexpression suppressed expression marker genes at messenger RNA (mRNA) protein...
The TORC2 gene is a member of the transducer regulated cyclic adenosine monophosphate (cAMP) response element binding protein family, which plays key role in metabolism and adipogenesis. In present study, we confirmed bovine preadipocyte proliferation through cell cycle staining flow cytometry, counting assay, 5-ethynyl-2′-deoxyuridine (EdU), mRNA expression analysis proliferation-related marker genes. addition, Oil red O analysis, immunofluorescence adiponectin, level lipid related genes...
Circular RNAs (circRNAs) are a class of endogenous non-coding (ncRNAs) involved in regulating skeletal muscle development by sponging miRNAs. In this study, we found that the circMYL1 expression was down-regulated during myoblast proliferation, while gradually up-regulated differentiation. The potential role identified proliferation bovine through mRNA and protein marker genes (PCNA, CyclinD1, CDK2), cell counting kit-8 assay, flow cytometry analysis, 5-ethynyl 2′-deoxyuridine (EdU) assay....
Abstract The proliferation and differentiation of myoblasts are essential for the regeneration development skeletal muscles. However, process muscle in cattle is complex needs to be further investigated. microRNAs (miRNAs) endogenous, small noncoding RNAs that play a critical role during development. In this study, we evaluated function miR‐885 cattle. results found expression was gradually upregulated myoblast proliferation, whereas progressively downregulated differentiation....
Copy number variations (CNVs) are gains and losses of genomic sequence more than 50 bp between two individuals a species. Also, CNV is considered to be one the main elements affecting phenotypic diversity evolutionary adaptation animals. ORMDL sphingolipid biosynthesis regulator 1 (ORMDL1) protein-coding gene associated with diseases development. In our study, polymorphism ORMDL1 copy numbers in four Chinese sheep breeds (abbreviated CK, HU, STH, LTH) was detected. addition, we analyzed...
Prolactin is a highly versatile pituitary hormone with multiple biological functions. PRL expression regulated by POU1F1 and the prophet of (PROP1). The aim this study was to investigate indel variations in ovine directly related (PROP1 POU1F1) genes, their associations growth traits Luxi Blackhead (LXBH) sheep. A monomorphism PROP1 one novel 23-bp insertion mutation gene, were identified LXBH 23 bp within gene significantly associated several body measurements (e.g., weight, height) sheep...
Abstract Background Circular RNAs (circRNAs) are a class of endogenous non-coding (ncRNAs) involved in regulating skeletal muscle development by sponging microRNAs (miRNAs). This study aimed to define the circ MYL1 molecular mechanisms regulation bovine myogenesis and disclose its regulatory mechanism through miR-2400 interaction. Methods The potential role was identified proliferation myoblast mRNA protein expression marker genes (PCNA, CyclinD1, CDK2) , cell counting kit-8 (CCK8) assay,...