A5066425041- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Viral Infections and Vectors
- interferon and immune responses
- Vector-Borne Animal Diseases
- Microbial infections and disease research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- Animal Genetics and Reproduction
- Parvovirus B19 Infection Studies
Beijing University of Chemical Technology
2019
Harbin Veterinary Research Institute
2009-2018
Chinese Academy of Agricultural Sciences
2009-2018
Northeast Agricultural University
2007-2018
State Key Laboratory of Veterinary Biotechnology
2011
Harbin Normal University
2011
Heilongjiang Academy of Land Reclamation Sciences
2007-2009
Henan University of Science and Technology
2009
Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Institute
2008
Xinjiang Agricultural University
2008
The genomic sequences of severe acute respiratory syndrome coronaviruses from human and palm civet the 2003/2004 outbreak in city Guangzhou, China, were nearly identical. Phylogenetic analysis suggested an independent viral invasion animal to this new episode. Combining all existing data but excluding singletons, we identified 202 single-nucleotide variations. Among them, 17 are polymorphic civets only. ratio nonsynonymous/synonymous nucleotide substitution collected 1 yr apart different...
Using three different assays, we examined 103 serum samples collected from civet farms and a market in China June 2003 January 2004. While civets on were largely free SARS-CoV infection, approximately 80% of the animals one animal Guangzhou contained significant levels antibody to SARS-CoV, which suggests no widespread infection among resident farms, might be associated with trading activities under conditions overcrowding mixing various species.
Five strains of infectious bronchitis virus (IBV) were isolated from five layer flocks that had nephropathogenic infection in four provinces China. Among them, three the been vaccinated against bronchitis. Virulence studies indicated Chinese IBV isolates caused 10 to 30% mortality 15-day-old specific pathogen free chickens and gross lesions mainly confined kidneys all dead chickens. Two oligonucleotide pairs, S1Uni2 S1Oligo3' or S1Oligo5' S1Oligo3', used after propagation embryonated eggs...
Coronavirus-like viruses, designated peafowl/China/LKQ3/2003 (pf/CH/LKQ3/03) and teal/China/LDT3/2003 (tl/CH/LDT3/03), were isolated from a peafowl teal during virological surveillance in Guangdong province, China. Partial genomic sequence analysis showed that these isolates had the S-3-M-5-N gene order is typical of avian coronaviruses. The spike, membrane nucleocapsid protein genes pf/CH/LKQ3/03 >99 % identity to those infectious bronchitis coronavirus H120 vaccine strain (Massachusetts...
Severe acute respiratory syndrome (SARS) was caused by a novel virus now known as SARS coronavirus (SARS-CoV). The discovery of SARS-CoV-like viruses in masked palm civets (Paguma larvata) raises the possibility that play role SARS-CoV transmission. To test susceptibility to experimental infection different isolates, 10 were inoculated with two human isolates SARS-CoV, BJ01 (with 29-nucleotide deletion) and GZ01 (without deletion). All animals displayed clinical symptoms, such fever,...
Twenty-six avian infectious bronchitis (IB) viruses (IBV) were isolated from outbreaks in chickens China between 1995 and 2004. They characterized by comparison with twenty-six Chinese reference strains five other IBV strains. IBVs, which mainly nephropathogenic, placed into seven genotypes. Fourteen isolates genotype I, having small evolutionary distances each other. Genotype II included 6 that the 1990s China. III consisted of eight showed close relationship Korean isolates. Another...
Abstract Background Infectious bronchitis virus (IBV), a prototype of the Coronaviridae family, is an economically important causative agent infectious in chickens and causes acute highly contagious upper respiratory tract infections that may lead to nephritis. However, molecular antiviral mechanisms IBV infection remain poorly understood. In this study, we conducted global gene expression profiling chicken kidney tissue after nephropathogenic better understand interactions between host...
Some of the structural proteins severe acute respiratory syndrome-associated coronavirus (SARS-CoV) carry major epitopes involved in virus neutralization and are essential for induction protective humoral responses development an effective vaccine. Rabbit antisera were prepared using full-length N M eight expressed fragments covering S protein. Antisera to found have different neutralizing titres towards SARS-CoV infection vivo, ranging from 1:35 1:128. Antiserum protein did not contain...
Seventy-eight isolates of avian infectious bronchitis virus (IBV) were obtained from different field outbreaks in China 2009 and genotyped with 34 reference strains. Four genotypes IBV three new identified by phylogenetic analysis BLAST searches the entire S1 gene. The results showed that most strains have circulated recent years belong to genotype QX-like strains, they could be grouped further into two clusters, regardless level genetic variation displayed. A study pathogenicity used...
Infectious bursal disease virus (IBDV) causes an economically significant of chickens worldwide. The emergence very virulent IBDV (vvIBDV) has brought more challenges for effective prevention this disease. molecular basis the virulence vvIBDV is not fully understood. In study, 20 strains were analysed phylogenically and clustered in three branches based on their full-length B segments. amino acid triplet located at positions 145/146/147 VP1 was found highly conserved branch I non-vvIBDVs as...
The entire S1 protein genes of eight infectious bronchitis (IB) vaccine strains used in China were compared with those the IB virus isolates present field China. nucleotide and amino acid similarities between strain, tl/CH/LDT3/03, which was isolated from a teal (Anas sp.), not more than 81.1% 79.2%, respectively. Phylogenetic analysis based on showed that vaccines belonged to different clusters larger evolutionary distances, indicated they genotypes. Four out vaccines, addition...
To monitor and study the molecular epidemiology, evolution pathogenicity of infectious bronchitis viruses (IBVs) in China recent years further our knowledge IBVs.Thirty-seven IBV isolates were isolated from commercial chickens China. The characterized by RT-PCR, sequencing, typing analyzing entire S1 gene. In addition, 4 selected used to experimentally infect specific pathogen-free their pathogenicity.Three types have been cocirculating chicken flocks years. Unique insertions deletions...