A5080584427- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Rare Diseases
- Pancreatic function and diabetes
- Cancer-related molecular mechanisms research
- vaccines and immunoinformatics approaches
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Genomics and Phylogenetic Studies
- CAR-T cell therapy research
- Pancreatitis Pathology and Treatment
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Bioinformatics and Genomic Networks
- Epigenetics and DNA Methylation
- Cell Image Analysis Techniques
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
German Cancer Research Center
2014-2019
Heidelberg University
2014-2019
European Molecular Biology Laboratory
2018-2019
European Molecular Biology Laboratory
2019
Institute of Applied Mathematics
2016-2017
For many diseases there are delays in diagnosis due to a lack of objective biomarkers for disease onset. Here, 41,931 individuals from the United Kingdom Biobank Pharma Proteomics Project, we integrated measurements ~3,000 plasma proteins with clinical information derive sparse prediction models 10-year incidence 218 common and rare (81-6,038 cases). We then compared developed using proteomic data either basic alone or combined 37 assays. The predictive performance including as few 5 20 was...
ABSTRACT New neurons are continuously generated in the dentate gyrus of adult hippocampus. This continuous supply newborn is important to modulate cognitive functions. Yet number declines with age. Increasing Wnt activity upon loss dickkopf 1 can counteract both decline and age-related decline. However, precise cellular changes underlying or its rescue fundamentally not understood. The present study combines a mathematical model experimental data address features controlling neural stem cell...
Abstract Background For many diseases there are delays in diagnosis due to a lack of objective biomarkers for disease onset. Whether measuring thousands proteins offers predictive information across wide range is unknown. Methods In 41,931 individuals from the UK Biobank Pharma Proteomics Project (UKB-PPP), we integrated ∼3000 plasma with clinical derive sparse prediction models 10-year incidence 218 common and rare (81 – 6038 cases). We compared based on a) basic alone, b) + 37 biomarkers,...
In the adult hippocampus, neurogenesis-the process of generating mature granule cells from neural stem cells-occurs throughout entire lifetime. order to investigate involved regulatory mechanisms, knockout (KO) experiments, which modify dynamic behaviour this process, were conducted in past. Evaluating these KOs is a non-trivial task owing complicated nature hippocampal neurogenic niche. study, we model neurogenesis as multicompartmental system ordinary differential equations based on...
Abstract Gene knockouts (KOs) are efficiently engineered through CRISPR-Cas9-induced frameshift mutations. While DNA editing efficiency is readily verified by sequencing, a systematic understanding of the protein elimination has been lacking. Here, we devised an experimental strategy combining RNA-seq and triple-stage mass spectrometry (SPS-MS3) to characterize 193 genetically deletions targeting 136 distinct genes generated CRISPR-induced frameshifts in HAP1 cells. We observed residual...
Abstract The poor immunogenicity of pancreatic tumors makes them particularly difficult to treat. Standard chemotherapies and single agent immunotherapies have had notoriously little success in this arena. Oncolytic virus therapy has the potential enhance penetration immunotherapeutically-delivered CAR T cells into tumor improve treatment outcomes. We evaluate by combining two different mathematical approaches: an ordinary differential equation model simulate population level response...
In the adult hippocampus, neural stem cells (NSCs) continuously produce new neurons that integrate into neuronal network to modulate learning and memory. The amount quality of newly generated decline with age, which can be counteracted by increasing intrinsic Wnt activity in NSCs. However, precise cellular changes underlying this age-related or its rescue through remain unclear. present study combines development a mathematical model experimental data address features controlling cell...
SUMMARY Cells constantly survey a complex set of inputs that is processed by the intracellular signaling network, but little known how cells integrate input information from more than one cue. We employed FRET biosensor-based imaging platform to study effect combinatorial growth factor levels on network in human cells. found pairwise stimuli caused distinct concentration- and ratio-dependent states through signatures such as antagonism, additivity synergy. The unique correlated with...