A5031019393- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Quantum Dots Synthesis And Properties
- Electromagnetic Fields and Biological Effects
- Biofuel production and bioconversion
- Cellular transport and secretion
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Biological Research and Disease Studies
- Carcinogens and Genotoxicity Assessment
- Graphene and Nanomaterials Applications
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Biomedical and Engineering Education
- Genomics and Rare Diseases
- Endoplasmic Reticulum Stress and Disease
- Hippo pathway signaling and YAP/TAZ
- Mobile Health and mHealth Applications
- Biochemical and Molecular Research
- Social Media in Health Education
National Research Council
2022-2024
Istituto per il Rilevamento Elettromagnetico dell'Ambiente
2023-2024
University of Campania "Luigi Vanvitelli"
2019-2023
Istituto di Chimica Biomolecolare
2019-2023
University of Lisbon
2022-2023
National Academies of Sciences, Engineering, and Medicine
2022
University of Padua
2020
University of Trento
2019
Institute of Applied Science and Intelligent Systems
2017-2019
Indium phosphide quantum dots (QDs) have emerged as a new class of fluorescent nanocrystals for manifold applications, from biophotonics to nanomedicine. Recent efforts in improving the photoluminescence yield, chemical stability and biocompatibility turned them into valid alternative well established Cd-based nanocrystals. In vitro studies provided first evidence lower toxicity In-based QDs. Nonetheless, an urgent need exists further assessment potential toxic effects vivo. Here we use...
Fabry disease is a lysosomal storage caused by mutations in the GLA gene that encodes alpha-galactosidase (AGAL). The causes abnormal globotriaosylceramide (Gb3) lysosomes. Variants responsible for genotypic spectrum of include abolish enzymatic activity and those cause protein instability. latter can be successfully treated with small molecules either bind stabilize AGAL or indirectly improve its cellular activity. This paper describes first attempt to reposition curcumin, nutraceutical,...
Fabry disease is caused by a deficiency of lysosomal alpha galactosidase and has very large genotypic phenotypic spectrum. Some patients who carry hypomorphic mutations can benefit from oral therapy with pharmacological chaperone. The drug requires precise regimen because it reversible inhibitor alpha-galactosidase. We looked for molecules that potentiate this chaperone, among drugs have already been approved other diseases. tested candidate in fibroblasts derived patient carrying deletion...
A large number of mutations causing PMM2-CDG, which is the most frequent disorder glycosylation, destabilize phosphomannomutase2. We looked for a pharmacological chaperone to cure starting from structure natural ligand phosphomannomutase2, α-glucose-1,6-bisphosphate. The compound, β-glucose-1,6-bisphosphate, was synthesized and characterized via 31P-NMR. β-glucose-1,6-bisphosphate binds its target enzyme in silico. binding induces conformational change that predicted by program PELE...
Water ecosystems represent main targets of unintentional contamination nanomaterials, due to industrial waste or other anthropogenic activities. Nanoparticle insult living organisms may occur in a sequential way, first by chemical interactions the material with target membrane, then progressive internalisation and interaction cellular structures organelles. These events trigger signal transduction, through which cells modulate molecular pathway order respond survive external elicitation....
The most common cause of human congenital disorders glycosylation (CDG) are mutations in the phosphomannomutase gene PMM2, which affect protein N -linked glycosylation. yeast SEC53 encodes a homolog PMM2 . We evolved 384 populations harboring one two human-disease-associated alleles, sec53- V238M and sec53 -F126L, or wild-type find that after 1000 generations, compensate for slow-growth phenotype associated with alleles. Through whole-genome sequencing we identify compensatory mutations,...
The 3,3′,5,5′-tetrachloro-2-iodo-4,4′-bipyridine structure is proposed as a novel chemical scaffold for the design of new transthyretin (TTR) fibrillogenesis inhibitors. In frame proof-of-principle exploration, four chiral 3,3′,5,5′-tetrachloro-2-iodo-2′-substituted-4,4′- bipyridines were rationally designed and prepared from simple trihalopyridine in three steps, including Cu-catalysed Finkelstein reaction to introduce iodine atoms on heteroaromatic scaffold, Pd-catalysed coupling install...
Abstract In our previous studies, we demonstrated that 20 h pre‐exposure of SH‐SY5Y human neuroblastoma cells to 1950 MHz, UMTS signal, at specific absorption rate 0.3 and 1.25 W/kg, was able reduce the oxidative DNA damage induced by a subsequent treatment with menadione in alkaline comet assay while not inducing genotoxicity per se. this study, same cell model used test experimental conditions setting different radiofrequency exposure duration timing along 72 culture period. The results...
Abstract This study aims to investigate the cellular effects of radiofrequency exposure, 1950 MHz, long‐term evolution (LTE) signal, administered alone and in combination with mitomycin‐C (MMC), a well‐known cytotoxic agent. Chinese hamster lung fibroblast (V79) cells were exposed/sham exposed waveguide‐based system under strictly controlled conditions both electromagnetic environmental parameters, at specific absorption rate (SAR) 0.3 1.25 W/kg. Chromosomal damage (micronuclei formation),...
Bioinformatics has pervaded all fields of biology and become an indispensable tool for almost research projects. Although teaching bioinformatics been incorporated in traditional life science curricula, practical hands-on experiences tight combination with wet-lab experiments are needed to motivate students.We present a tutorial that starts from problem: finding novel enzymes marine environments. First, we introduce the idea metagenomics, recent approach extends biotechnology non-culturable...
ABSTRACT The most common cause of human congenital disorders glycosylation (CDG) are mutations in the phosphomannomutase gene PMM2 , which affect protein N -linked glycosylation. yeast SEC53 encodes a nearly-identical homolog . We evolved 384 populations harboring one two human-disease-associated alleles, sec53 -V238M and -F126L, or wild-type find that after 1,000 generations, compensate for slow-growth phenotype associated with alleles. Through whole-genome sequencing we identify...