A5019772585- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Transplantation: Methods and Outcomes
- Cancer, Stress, Anesthesia, and Immune Response
- Immune Cell Function and Interaction
- Cystic Fibrosis Research Advances
- Congenital heart defects research
- Respiratory viral infections research
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Fibroblast Growth Factor Research
- Congenital Diaphragmatic Hernia Studies
- Cancer Immunotherapy and Biomarkers
- Hereditary Neurological Disorders
- Extracellular vesicles in disease
- Polyomavirus and related diseases
- Ultrasound in Clinical Applications
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Neurogenetic and Muscular Disorders Research
- Telomeres, Telomerase, and Senescence
- Gut microbiota and health
University of Verona
2011-2021
KU Leuven
2014-2016
Universitair Ziekenhuis Leuven
2014
Brigham and Women's Hospital
2012
Clinical-grade mesenchymal stromal cells (MSCs) are usually expanded from bone marrow (BMMSCs) or adipose tissue (ADSCs) using processes mainly differing in the use of fetal calf serum (FCS) human platelet lysate (PL). We aimed to compare immune modulatory properties clinical-grade MSCs a combination fully standardized vitro assays. BMMSCs with FCS (BMMSC-FCS) PL (BMMSC-PL), and ADSC-PL were analyzed quantitative phenotypic functional experiments, including their capacity inhibit...
Epithelial-to-mesenchymal transition (EMT) has a central role in cancer progression and metastatic dissemination may be induced by local inflammation. We asked whether the inflammation-induced acquisition of mesenchymal phenotype neoplastic epithelial cells is associated with onset stromal cell-like immune-regulatory properties that enhance tumour immune escape.Cell lines lung adenocarcinoma (A549), breast (MCF7) hepatocellular carcinoma (HepG2) were co-cultured T, B NK before after EMT...
Rationale: Hypoxia favors stem cell quiescence, whereas normoxia is required for activation, but whether cardiac (CSC) function regulated by the hypoxic/normoxic state of currently unknown. Objective: A balance between hypoxic and normoxic CSCs may be present in young heart, although this homeostatic control disrupted with aging. Defects tissue oxygenation occur old myocardium, phenomenon expand pool CSCs, which are no longer involved myocyte renewal. Methods Results: Here, we show that...
Allogeneic stem cell (SC)-based therapy is a promising tool for the treatment of range human degenerative and inflammatory diseases. Many reports highlighted immune modulatory properties some SC types, such as mesenchymal stromal cells (MSCs), but comparative study with SCs different origin, to assess whether regulation general property, still lacking. To this aim, we applied highly standardized methods employed MSC characterization compare immunological bone marrow-MSCs, olfactory...
Mesenchymal stromal cells (MSCs) reside in many organs including lung, as shown by their isolation from fetal lung tissues, bronchial compartment, bronchial-alveolar lavage and transplanted tissues. It is still controversial whether MSCs can undergo mesenchymal-to-epithelial-transition (MET) possess immune regulatory properties. To this aim, we isolated, expanded characterized normal adult human (lung-hMSCs) compared with bone marrow-derived (BM-hMSCs). Our results show that lung-MSCs at the...
Background Evaluation of cystic fibrosis transmembrane conductance regulator (CFTR) functional activity to assess new therapies and define diagnosis (CF) is cumbersome. It known that leukocytes express detectable levels CFTR but the molecule has not been characterized in these cells. In this study we aim at setting up validating a blood test evaluate expression function leukocytes. Description Western blot, PCR, immunofluorescence cell membrane depolarization analysis by single-cell...
PREP1 (PKNOX1) maps in the Down syndrome (DS) critical region of chromosome 21, is overexpressed some DS tissues and might be involved phenotype. By using fibroblasts from patients by overexpressing Prep1 F9 teratocarcinoma Prep1i/i MEF to single out role protein, we report that excess increases sensitivity cells genotoxic stress extent apoptosis directly correlates with level Prep1. The apoptotic response Prep1-overexpressing mediated pro-apoptotic p53 protein show a direct target Prep1, as...
In this paper, we introduce the concept of random time changes in dynamical systems. The sub- ordination principle may be applied to study long behavior We show, under certain assumptions on class time, that subordinated system exhibits a slower decay which is determined by characteristics. Along path asymp- totic, change reflected new velocity resulting dynamics.