A5061623444- Hepatocellular Carcinoma Treatment and Prognosis
- Pulmonary Hypertension Research and Treatments
- Advanced Radiotherapy Techniques
- Prostate Cancer Diagnosis and Treatment
- Medical Imaging and Pathology Studies
- Nitric Oxide and Endothelin Effects
- Medical Imaging Techniques and Applications
- Cardiac Imaging and Diagnostics
- Neonatal Respiratory Health Research
- Lung Cancer Diagnosis and Treatment
- Advanced X-ray and CT Imaging
- Radiomics and Machine Learning in Medical Imaging
- Pregnancy and preeclampsia studies
- Tracheal and airway disorders
- MRI in cancer diagnosis
- Ultrasound and Hyperthermia Applications
- Renal cell carcinoma treatment
- Radiopharmaceutical Chemistry and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Anatomy and Medical Technology
- Virus-based gene therapy research
- Venous Thromboembolism Diagnosis and Management
- Bladder and Urothelial Cancer Treatments
- Nanoplatforms for cancer theranostics
- Advanced MRI Techniques and Applications
National Institutes of Health Clinical Center
2017-2025
National Institutes of Health
2016-2024
National Cancer Institute
2018-2020
Kelly Services (United States)
2018
Children's Hospital of Wisconsin
2012-2013
Medical College of Wisconsin
2004-2013
Pediatrics and Genetics
2011
Persistent pulmonary hypertension of newborn (PPHN) is associated with decreased NO release and impaired vasodilation. We investigated the hypothesis that increased superoxide (O(2)(*-)) by an uncoupled endothelial nitric oxide synthase (eNOS) contributes to vasodilation in PPHN. response isolated arteries NOS agonist ATP donor S-nitroso-N-acetylpenicillamine (SNAP) fetal lambs PPHN induced prenatal ligation ductus arteriosus sham-ligated controls presence or absence antagonist...
A rapid increase in the synthesis and release of nitric oxide (NO) facilitates pulmonary vasodilation that occurs during birth-related transition. Alteration this transition persistent hypertension newborn (PPHN) is associated with impaired function endothelial synthase (eNOS) an oxidative stress. We investigated hypothesis a decrease expression activity mitochondrial localized manganese superoxide dismutase (MnSOD) artery cells (PAEC) increases stress impairs eNOS PPHN. isolated PAEC...
Persistent pulmonary hypertension of newborn (PPHN) is associated with impaired vasodilation at birth. Previous studies demonstrated that a decrease in angiogenesis contributes to this failure postnatal adaptation. We investigated the hypothesis oxidative stress from NADPH oxidase (Nox) PPHN. PPHN was induced fetal lambs by ductus arteriosus ligation 85% term gestation. Pulmonary artery endothelial cells (PAEC) (HTFL-PAEC) or control (NFL-PAEC) were compared for their angiogenic activities...
Abstract Purpose This study aimed to describe the workflow and evaluate accuracy of a novel smartphone augmented reality (AR) application that includes an integrated needle guide, in phantom. Materials Methods A cover with guide was designed 3D-printed. An AR for percutaneous developed, which projected path based on rigid guide. After planning using this tool, operator could place through reach target. Six lesions out-of-plane entry points were targeted abdominal Timing placements measured...
Purpose To correlate bead location and attenuation on CT images with the quantity distribution of drug delivered to liver following transarterial chemoembolization (TACE) radiopaque drug-eluting beads (DEB) in a rabbit tumor model. Materials Methods All procedures were performed protocol approved by Institutional Animal Care Use Committee. TACE was rabbits (n = 4) bearing VX2 tumors using DEB (70-150 µm) loaded doxorubicin (DOX). Livers resected 1 hour after embolization, immediately frozen,...
Heat-induced destruction of cancer cells via microwave ablation (MWA) is emerging as a viable treatment primary and metastatic liver cancer. Prediction the impacted zone where cell death occurs, especially in presence vasculature, challenging but may be achieved biophysical modeling. To advance characterize thermal MWA for focal treatment, an vivo method experimental dataset were created assessment models designed to dynamically predict parameters, given delivery device, power, location,...
Persistent pulmonary hypertension of the newborn (PPHN) is associated with decreased blood vessel density that contributes to increased vascular resistance. Previous studies showed uncoupled endothelial nitric oxide (NO) synthase (eNOS) activity and NADPH oxidase resulted in marked decreases NO bioavailability impaired angiogenesis PPHN. In present study, we hypothesize loss tetrahydrobiopterin (BH4), a critical cofactor for eNOS, induces eNOS impairs Pulmonary artery cells (PAEC) isolated...
Purpose: To evaluate lyso-thermosensitive liposomal doxorubicin (LTLD, ThermoDox®) in combination with loco-regional mild hyperthermia (HT) for targeted drug delivery to the bladder wall and potential treatment of cancer.Material methods: Porcine vivo studies were performed following groups: (i) intravenous (IV) LTLD (LTLD + HT); (ii) IV (DOX) (IV DOX HT) (iii) without – HT). Drug formulations delivered via 30 min infusion coinciding 1-h irrigation (45 °C water HT groups, 37 non-HT group),...
The purine nucleotide ATP mediates pulmonary vasodilation at birth by stimulation of P2Y receptors in the circulation. specific circulation and segmental distribution their responses remain unknown. We investigated effects nucleotides, ATP, ADP, AMP, pyrimidine UTP, UDP, UMP, juvenile rabbit arteries for functional characterization receptors. also studied expression receptor subtypes role nitric oxide (NO), prostaglandins, cytochrome P-450 metabolites response to ATP. In conduit size...
Background: Endobronchial navigation is performed in a variety of ways, none which are meeting all the clinicians' needs required to reach diagnostic success every patient. We sought characterize precurved and steerable guiding sheaths (GS) endobronchial targeting for lung biopsy using cone beam computed tomography (CBCT) based augmented fluoroscopy (AF) image guidance. Methods: Four GS (EdgeTM 45, 90, 180, 180EW, Medtronic) two [6.5 F Destino Twist (DT), Oscor; 6 Morph, BioCardia] were...
Purpose: A novel grid-template-mimicking MR-compatible robot was developed for in-gantry MRI-guided focal laser ablation of prostate cancer. Method: substantially compact designed and prototyped to meet lithotomy ergonomics allow accommodation in the perineum. The controller software reconfigured integrated with custom-designed navigation multi-focal software. Three experiments were conducted: (1) free space accuracy test; (2) phantom study under computed tomography (CT) guidance...
PurposeAn augmented reality (AR) system was developed to facilitate free-hand real-time needle guidance for transperineal prostate (TP) procedures and overcome the limitations of a traditional grid.ApproachThe HoloLens AR enables superimposition annotated anatomy derived from preprocedural volumetric images onto patient addresses most challenging part TP by providing tip localization depth visualization during insertion. The accuracy, or image overlay accuracy (n = 56), targeting 24) were...
Background: Woodchucks chronically infected with woodchuck hepatitis virus (WHV), which resembles human B virus, develop spontaneous hepatic tumors and may be an important biological immunological model for HCC.Nonetheless, this requires further validation to fully realize its translational potential.Methods: at birth WHV that had developed HCC (n=12) were studied.Computed tomography, ultrasound, magnetic resonance imaging performed under anesthesia.LI-RADS scoring correlative histologic...
To evaluate plasma pharmacokinetics of a small molecule PD-L1 checkpoint inhibitor following embolization woodchuck hepatocellular carcinoma (HCC) with radiopaque drug-eluting beads (DEB). Woodchucks HCC (N=8) underwent transarterial DEB (40-90 μm, LC Bead LUMI) loaded inhibitor, BMS-202. Peripheral blood samples were collected during selective hepatic and for 4 hours arterial stasis. Following euthanasia, the liver was resected micro-CT performed to visualize distribution. BMS-202 measured...