A5015006634- Advanced Radiotherapy Techniques
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Prostate Cancer Treatment and Research
- Medical Imaging Techniques and Applications
- Radiation Dose and Imaging
- Muscle metabolism and nutrition
- Vitamin D Research Studies
- Endometrial and Cervical Cancer Treatments
- Ultrasound and Hyperthermia Applications
- RNA Research and Splicing
- Exercise and Physiological Responses
- Radiation Effects and Dosimetry
- Growth Hormone and Insulin-like Growth Factors
- Bone Metabolism and Diseases
- Sarcoma Diagnosis and Treatment
- Advanced X-ray and CT Imaging
- Surgical site infection prevention
- Breast Cancer Treatment Studies
- Metabolism, Diabetes, and Cancer
- Biotin and Related Studies
- Radiation Detection and Scintillator Technologies
- Advances in Oncology and Radiotherapy
Karolinska University Hospital
2015-2023
Karolinska Institutet
2014-2023
Moffitt Cancer Center
2010-2022
Hagerstown Community College
2020
Florida Hospital Cancer Institute
2017
Loughborough University
2013-2014
University of South Florida
2002-2012
Manchester Metropolitan University
2006-2011
University of Bedfordshire
2011
Great Ormond Street Hospital
2006
Presence of the vitamin D receptor and direct effects on proliferation differentiation muscle precursor cells have been demonstrated in animal models. However, mechanisms actions human skeletal muscle, presence adult remain to be established. Here, we investigated role at various stages differentiation. We demonstrate that components D-endocrine system are readily detected but low nondetectable lack ability convert inactive D-metabolite 25-hydroxy-vitamin D3 active 1α,25-dihydroxy-vitamin...
Skeletal muscle adaptations to exercise have been associated with a range of health-related benefits, but cell type-specific within the are incompletely understood. Here we use single-cell sequencing determine effects on cellular composition and processes in human skeletal before after intense exercise. Fifteen clusters originating from six different populations were identified. Most remained quantitatively stable exercise, large transcriptional response was observed mesenchymal,...
The dose‐rate dependence of commercially available diode detectors was measured under both high instantaneous (pulsed) and low dose rate (continuous, Co‐60) radiation. in an acrylic miniphantom at a 5‐cm depth collimator setting, by varying source–to‐detector distance (SDD) between least 80 200 cm. ratio normalized reading to ion chamber (both used determine sensitivity for pulsed continuous radiation different SDD. inverse the is defined as SDD correction factor (SDD CF). increased with...
Sarcopenic obesity is characterised by high fat mass, low muscle mass and an elevated inflammatory environmental milieu. We therefore investigated the effects of cytokine TNF-α (aging/obesity) saturated fatty acid, palmitate (obesity) on skeletal cells in presence/absence EPA, a-3 polyunsaturated acid with proposed anti-inflammatory, anti-obesity activities. In present study we show that was lipotoxic, inducing levels cell death blocking myotube formation. Cell under these conditions...
The energy dependence of commercially available diode detectors was measured for nominal accelerating potential ranging between Co-60 and 17 MV. measurements were performed in a liquid water phantom at 5 cm depth 10 x cm2 collimator setting source-to-detector distance 100 cm. response (nC/Gy) normalized to beam after corrections the dose rate temperature dependences each diode. dependence, calculated by taking percent difference maximum minimum sensitivity beam, varied 39% n-type Isorad Red,...
Sirtuin 1 also known as NAD-dependent deacetylase sirtuin 1, is a protein that in humans encoded by the Sirt1 gene. an enzyme deacetylates proteins contribute to cellular regulation and key regulator of cell defenses survival response stress. Deletion abolishes increase lifespan induced calorie restriction or sublethal cytokine stress, indicating promotes longevity survival. We have demonstrated administration dose tumour necrosis factor-α (TNF-α; 1.25 ng ml(-1)) inhibits myotube formation,...
Temperature dependence of commercially available n ‐ and p ‐type diodes were studied experimentally under both high instantaneous dose rate (pulsed) low (continuous) radiation. The sensitivity versus temperature was measured at or 100 cm, 5 cm depth in a water phantom between 10 °C 35 °C. response linear for all the diode detectors. coefficient (or variation with temperature, svwt) independent preirradiated diodes. They QED ‐type, EDP Isorad diodes, respectively. unirradiated different...
The interaction effects of tumour necrosis factor-alpha (TNF-α) and interlukin-6 (IL-6) on skeletal muscle proliferation differentiation remains controversial. We therefore investigated the potential interactive TNF-α IL-6 murine C2 myoblast survival, proliferation. A novel unexpected positive temporal between cell growth was identified (90%), with maximal beneficial obtained in myoblasts treated (10 ng/ml) for 24 h prior to being dosed (2.5 a further h. This combined treatment significantly...
The concept of skeletal muscle homeostasis--often viewed as the net balance between two separate processes, namely protein degradation and synthesis--are not occurring independently each other, but are finely co-ordinated by a web intricate signalling networks.Using rodent cell lines we have investigated TNF-alpha/IGF-I interactions, in an attempt to mimic understand mechanisms underlying wasting process.When myoblast cells incubated with TNF-alpha (10 ng ml(- 1)) maximal damage (...
To examine if the physiological concentrations of both interleukin-6 (IL-6), in combination with IL-6 receptor (IL-6R), are able to stimulate glucose uptake human skeletal muscle and identify associated signalling pathways.Skeletal tissue (~60 mg) obtained from healthy female volunteers via biopsy was subjected incubation absence or presence insulin (60 µU/ml), recombinant (rhIL-6) (4 ng/ml) a rhIL-6 rhIL-6R (100 for 30 min, transport measured each incubation. Western blot analysis conducted...
Our previous work has established that angiotensin II is cardiotoxic. Here we sought to investigate whether skeletal muscle similarly susceptible damage. Male Wistar rats were either given a single subcutaneous injection of (range 1 microg kg-1 10 mg kg-1) or only the vehicle and killed 7 h later, implanted with preconditioned osmotic pumps dispensing day-1 9 18 later. Apoptotic (caspase 3 positive) myocytes counted on cryosections heart, soleus, tibialis anterior diaphragm muscle. Single...
Skeletal muscle is a post-mitotic tissue maintained by repair and regeneration through population of stem cell-like satellite cells. Following injury, cell proliferation mediated local signals ensuring sufficient progeny for repair. Age–related changes in cells as well to the systemic environment potentially impact on capacity generate an ageing organism resulting diminished regeneration. 'Rejuvenation' regenerative environmental stimuli effectors suggest that subset age-dependent may be...
Potential roles for undifferentiated skeletal muscle stem cells or satellite in hypertrophy and repair have been reported, however, the capacity, mode mechanisms underpinning migration not investigated. We hypothesised that damaged myoblasts would elicit a mesenchymal-like migratory response, which could be precisely tracked subsequently manipulated.We therefore established model of mechanical damage developed MATLAB(TM) tool to measure capacity non-subjective manner.Basal following was...
For successful growth and maintenance of primary myogenic cells in vitro, culture medium addition sera are the most important factors. At present it is not established as to what extent different origin composition, supplemented media or serum-free conditions influence myoblast function responses stimuli. By assessing markers proliferation, differentiation/fusion, quiescence, apoptosis protein synthesis aim current study was elucidate how human myoblasts myotubes modulated by commonly used...