A5048502906- Muscle metabolism and nutrition
- Sports Performance and Training
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Cardiovascular and exercise physiology
- Genetics and Physical Performance
- Pharmacological Effects and Assays
- Advanced Proteomics Techniques and Applications
- Genetic Neurodegenerative Diseases
- Ion channel regulation and function
- Mass Spectrometry Techniques and Applications
- Receptor Mechanisms and Signaling
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Effects of Exercise
- High Altitude and Hypoxia
- Thermoregulation and physiological responses
- Muscle activation and electromyography studies
- Cardiac Ischemia and Reperfusion
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Cardiac pacing and defibrillation studies
- Children's Physical and Motor Development
- Cardiomyopathy and Myosin Studies
Liverpool John Moores University
2016-2025
English Institute of Sport
2023
Maastricht University
2020
Maastricht University Medical Centre
2020
University of Liverpool
2020
University of Florida
2015
Elite athletes are particularly susceptible to sleep inadequacies, characterised by habitual short (<7 hours/night) and poor quality (eg, fragmentation). Athletic performance is reduced a night or more without sleep, but the influence on of partial restriction over 1–3 nights, real-world scenario, remains unclear. Studies investigating in often suffer from inadequate experimental control, lack females questions concerning validity chosen assessment tools. Research only scratches surface...
Intramuscular triglyceride (IMTG) utilization is enhanced by endurance training (ET) and linked to improved insulin sensitivity. This study first investigated the hypothesis that ET-induced increases in net IMTG breakdown sensitivity are related increased expression of perilipin 2 (PLIN2) 5 (PLIN5). Second, we hypothesized sprint interval (SIT) also promotes Sixteen sedentary males performed 6 weeks either SIT (4-6, 30 s Wingate tests per session, 3 days week(-1)) or ET (40-60 min...
No previous study has used proteomics to investigate the effects of exercise training on human skeletal muscle. Five recreationally active men completed a 6-wk programme involving three sessions per week, utilising six 1-min bouts at maximum oxygen uptake (V O(2)max) interspersed with 4 min 50% V O(2)max. Vastus lateralis was biopsied standardised times before and after intervention. Protein expression profiling performed using differential analysis 2-DE gels; complemented quantitative...
High levels of catecholamines are myotoxic but the relative amounts apoptosis and necrosis have not been established in vivo cardiac skeletal muscles. Immunohistochemistry was used to detect quantify myocyte-specific (myosin antibody vivo) (caspase-3 vitro) heart soleus muscles male Wistar rats that had received single subcutaneous injections isoprenaline over range 1 microg 5 mg [kg body weight (BW)](-1). Peak myocyte occurred 3-6 h after, 18 a injection (kg BW)(-1) vivo. In death mediated...
It is generally accepted that muscle adaptation to resistance exercise training underpinned by contraction-induced increased rates of protein synthesis and dietary availability.Utilising Dynamic Proteome Profiling (DPP), we investigated the contributions both breakdown changes in abundance on a protein-by-protein basis human skeletal muscle.Age-matched, overweight males consumed nine days high-fat, lowcarbohydrate (HFLC) diet during which time they either undertook three sessions (REX) or...
We have investigated the effects that sleep restriction (3-h during two consecutive nights) on an evening (17:00 h) submaximal weightlifting session; and whether this performance improves following a 1-h post-lunch powernap. Fifteen resistance-trained males participated in study. Before experimental protocol commenced, 1RM bench press inclined leg normative habitual were recorded. Participants familiarised with testing protocol, then completed three conditions nights of prescribed sleep: (i)...
We investigated the hypothesis that a daily-variation exists for 4-km time-trial, where standardised-approach has been employed. Twenty-six male students completed 30-s Wingate test, V˙O2 peak test: and then three familiarisations at 12:00 h. Two further time-trials counterbalanced start 07:30 or 17:30 h were undertaken. There was 72 between each session. Heart rate, rectal skin temperature values, rating-of-perceived-exertion, effort, pacing perception split-times measured every 1-km during...
Diurnal variations in time-trial performance have been shown people living normally, where a "standardised protocol" has employed to reduce bias. We tested the hypothesis that circadian variation exists for 10-km running laboratory-based time-trial, such standardised approach is used. Twelve recreationally active adult males were recruited. The participants completed three familiarisation time-trials best of their ability at self-selected pace and six 06:00, 10:00, 14:00, 18:00, 22:00 02:00...
Resistance training (RT) promotes muscle protein accretion and myofiber hypertrophy, driven by dynamic processes of synthesis degradation. While molecular studies have focused on acute signalling or long-term hypertrophy strength gains, a critical gap remains in understanding the intermediate adaptation. Acute does not always correlate directly with outcomes, highlighting need for time-course analysis abundance turnover rates. To address this, we utilised deuterium oxide labelling peptide...
Rugby players may repeatedly incur high-impact collisions that could predispose them to neurodegenerative conditions but the processes underlying heightened risk are currently unclear. This project investigates whether proteome of plasma extracellular vesicles (EV) carry putative diagnostic biomarkers indicate differences in across a rugby playing career. Twenty-four males were recruited, including eight academy (18+/- 1 y), professional (33 +/- 5 y) with >10-year career and CrossFit...
FLExDUX4 is a murine experimental model of facioscapulohumeral muscular dystrophy (FSHD) characterized by chronic, low levels leaky expression the human full-length double homeobox 4 gene (DUX4-fl). mice exhibit mild pathologies and functional deficits similar to people affected FSHD. Proteomic studies in FSHD could offer new insights into disease mechanisms underpinned post-transcriptional processes. We used mass spectrometry-based proteomics quantify abundance 1322 proteins triceps brachii...
Myocyte-specific necrosis in the heart and soleus muscle of adult male Wistar rats was investigated response to a single subcutaneous injection anabolic beta(2)-adrenergic receptor agonist clenbuterol. Necrosis immunohistochemically detected by administration myosin antibody 1 h before clenbuterol challenge quantified using image analysis. Clenbuterol-induced myocyte occurred against background zero damage control muscles. In heart, clenbuterol-induced not uniform, being more abundant left...
High doses of the β 2 -adrenergic receptor (AR) agonist clenbuterol can induce necrotic myocyte death in heart and slow-twitch skeletal muscle rat. However, it is not known whether this agent also apoptosis would occur at a lower dose than previously reported for necrosis. Male Wistar rats were given single subcutaneous injections clenbuterol. Immunohistochemistry was used to detect myocyte-specific (detected on cryosections via caspase 3 antibody confirmed with annexin V, single-strand DNA...
The lipid droplet (LD)-associated protein perilipin 2 (PLIN2) appears to colocalize with LDs in human skeletal muscle fibres, although the function of PLIN2 regulation intramuscular triglyceride (IMTG) metabolism is currently unknown. Here we investigated hypothesis that presence related IMTG utilisation during exercise. We therefore measured exercise-induced changes and distribution their colocalization. Muscle biopsies from vastus lateralis were obtained seven lean, untrained men (22 ±...
We report automated and time-efficient (2 h per sample) profiling of muscle using ultra-performance LC coupled directly with high-definition MS (HDMS(E)). Soluble proteins extracted from rat gastrocnemius (n = 10) were digested trypsin analyzed in duplicate a 90 min RPLC gradient. Protein identification label-free quantitation performed HDMS(E) spectra Progenesis QI for Proteomics software. In total 1514 identified. Of these, 811 had at least three unique peptides subsequently used to assess...
Muscle fiber composition is associated with physical performance, endurance athletes having a high proportion of slow-twitch muscle fibers compared to power athletes. Approximately 45% heritable, however, single nucleotide polymorphisms (SNP) underlying inter-individual differences in types remain largely unknown. Based on three whole genome SNP datasets, we have shown that the rs236448 A allele located near cyclin-dependent kinase inhibitor 1A (CDKN1A) gene was an increased Russian (n =...
Abstract Endurance training improves cardiac function and protects against heart disease. The rodent intensity‐controlled running model replicates endurance exercise in humans can be used to investigate molecular adaptations the heart. Rats ( n = 6, 280 ± 3 g) performed tests measure their peak oxygen uptake $\ { \dot V} O}_{ 2} peak}$ ) was prescribed at 70–75% for 30 min, 4 days/wk. Hearts were isolated h after a final test left ventricle proteomes compared weight‐matched control animals...