A5030375958- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Circadian rhythm and melatonin
- Dietary Effects on Health
- Exercise and Physiological Responses
- Spaceflight effects on biology
- Sports Performance and Training
- Advanced Proteomics Techniques and Applications
- Genetics and Physical Performance
- Cardiovascular and exercise physiology
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Protein Hydrolysis and Bioactive Peptides
- Genetics, Aging, and Longevity in Model Organisms
- Hormonal and reproductive studies
- Nutrition and Health in Aging
- Neurobiology and Insect Physiology Research
- Forensic Toxicology and Drug Analysis
University of Central Lancashire
2023-2024
Liverpool John Moores University
2016-2024
University of Florida
2020-2023
Cellular circadian clocks direct a daily transcriptional program that supports homeostasis and resilience. Emerging evidence has demonstrated age-associated changes in functions. To define age-dependent at the systems level, we profile transcriptome hypothalamus, lung, heart, kidney, skeletal muscle, adrenal gland three age groups. We find tissue-specific clock output changes. Aging reduces number of rhythmically expressed genes (REGs), indicative weakened control. REGs are enriched for...
For more than 20 years, physiologists have observed a morning-to-evening increase in human muscle strength. Recent data suggest that time-of-day differences are the result of intrinsic, nonneural, factors. We evaluate circadian clock sets from and mouse studies highlight possible mechanisms through which may contribute to strength outcomes.
Resistance training (RT) promotes muscle protein accretion and myofiber hypertrophy, driven by dynamic processes of synthesis degradation. While molecular studies have focused on acute signalling or long-term hypertrophy strength gains, a critical gap remains in understanding the intermediate adaptation. Acute does not always correlate directly with outcomes, highlighting need for time-course analysis abundance turnover rates. To address this, we utilised deuterium oxide labelling peptide...
Muscle adaptations to exercise are underpinned by alterations the abundance of individual proteins, which may occur through a change either synthesis or degradation each protein. We used deuterium oxide (2H2O) labeling and chronic low-frequency stimulation (CLFS) in vivo investigate synthesis, abundance, proteins during exercise-induced muscle adaptation. Independent groups rats received CLFS (10 Hz, 24 h/d) 2H2O for 0, 10, 20, 30 days. The extensor digitorum longus (EDL) was isolated from...
Differences in the protein composition of fast- and slow-twitch muscle may be maintained by different rates turnover. We investigated turnover soleus fast-twitch plantaris male Wistar rats (body weight 412 ± 69 g). Animals were assigned to four groups (n = 3, each), including a control group (0 d) three that received deuterium oxide (D2O) for either 10 days, 20 days or 30 days. D2O administration was initiated an intraperitoneal injection μL 99% D2O-saline per g body weight, provision 4%...
The turnover of muscle protein is responsive to different (patho)-physiological conditions but little known about the rate synthesis at level individual proteins or whether this varies between muscles. We investigated eight (actin, albumin, ATP synthase alpha, beta enolase, creatine kinase, myosin essential light chain, regulatory chain and tropomyosin) in extensor digitorum longus, diaphragm, heart soleus male Wistar rats (352 ± 30 g body weight). Animals were assigned four groups (n = 3,...
Time-of-day differences in acute exercise performance mice are well established with late active phase (afternoon) runners exhibiting significantly greater endurance compared to early (morning) runners. In this study, we asked if adaptations would be different when training for 6 weeks at two times of day, and corresponds steady state changes the peripheral tissue clocks. To address these questions, trained female PER2::Luciferase mice, same relative workload, either morning, ZT13, or...
Muscle atrophy exacerbates disease outcomes and increases mortality, whereas the preservation of skeletal muscle mass function play pivotal roles in ensuring long-term health overall quality-of-life. represents a significant clinical challenge, involving continued loss strength, which frequently accompany development numerous types cancer. Cancer cachexia is highly prevalent multifactorial syndrome, although one main causes cancer-related deaths, there are still no approved management...
The application of proteomic analysis to forensic skeletal remains has gained significant interest in improving biological and chronological estimations medico-legal investigations. To enhance the applicability these analyses casework, it is crucial maximize throughput proteome recovery while minimizing interoperator variability laboratory-induced post-translational protein modifications (PTMs). This work compared different workflows for extracting, purifying, analyzing bone proteins using...
Resistance training increases muscle size but little is known about the dynamic processes that underpin gains in mass. We investigated rat during 30 days of unilateral programmed resistance (PRT) using stable isotope (deuterium oxide; D2O) vivo and proteomics. Three-month-old, male Wistar rats (body weight 348 ± 20 g) were assigned to four groups (n = 4, each), including a control group experimental received D2O for 10, or days. Under ethically approved procedures, deuterium was...
In this investigation, we addressed the contribution of core circadian clock factor, BMAL1, in skeletal muscle to both acute transcriptional responses exercise and remodelling response training. Additionally, adopted a systems biology approach investigate how loss BMAL1 altered peripheral tissue homeostasis as well training adaptations iWAT, liver, heart, lung male mice.