A5046317623- Circadian rhythm and melatonin
- Ginseng Biological Effects and Applications
- Pharmacological Effects of Natural Compounds
- Neonatal Respiratory Health Research
- Congenital Diaphragmatic Hernia Studies
- Immune Cell Function and Interaction
- Antiplatelet Therapy and Cardiovascular Diseases
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Natural product bioactivities and synthesis
- Genetic and phenotypic traits in livestock
- Medical Imaging and Pathology Studies
- Sleep and related disorders
- Ruminant Nutrition and Digestive Physiology
- Essential Oils and Antimicrobial Activity
- Genetics, Aging, and Longevity in Model Organisms
- Food Quality and Safety Studies
- Botanical Research and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Fungal Biology and Applications
- Pulmonary Hypertension Research and Treatments
- Wnt/β-catenin signaling in development and cancer
- Chemical synthesis and alkaloids
- Dietary Effects on Health
- Magnolia and Illicium research
University of Florida
2020-2024
Florida College
2020-2024
Perinatal Institute
2022
Cincinnati Children's Hospital Medical Center
2013-2018
Kyungpook National University
2009-2014
Addis Ababa University
2011
Cellular circadian clocks direct a daily transcriptional program that supports homeostasis and resilience. Emerging evidence has demonstrated age-associated changes in functions. To define age-dependent at the systems level, we profile transcriptome hypothalamus, lung, heart, kidney, skeletal muscle, adrenal gland three age groups. We find tissue-specific clock output changes. Aging reduces number of rhythmically expressed genes (REGs), indicative weakened control. REGs are enriched for...
Epithelial-mesenchymal cell interactions and factors that control normal lung development are key players in injury, repair, fibrosis. A number of studies have investigated the roles sources epithelial progenitors during regeneration; such information, however, is limited fibroblasts. Thus, understanding origin, phenotype, fibroblast development, regeneration helps address these limitations. Using a combination platelet-derived growth factor receptor α-green fluorescent protein (PDGFRα-GFP)...
Ginsenosides are the main constituents for pharmacological effects of Panax ginseng. Such ginsenosides including cardioprotective and anti-platelet activities have shown stability bioavailability limitations. However, information on activity ginsenoside-Rp1 (G-Rp1), a stable derivative ginsenoside-Rg3, is scarce. We examined ability G-Rp1 to modulate agonist-induced platelet activation.G-Rp1 in vitro ex vivo platelet-aggregation, granule-secretion, [Ca(2+) ](i) mobilization, integrin-α(IIb)...
Many studies have investigated the source and role of epithelial progenitors during lung development; such information is limited for fibroblast populations their complex in developing lung. In this study, we characterized spatial location, mRNA expression Immunophenotyping PDGFRα+ fibroblasts sacculation alveolarization. Confocal microscopy identified association PDGFRα expressing with proximal cells branching bronchioles dilating acinar tubules at E16.5; distal terminal saccules E18.5;...
In mammals, the circadian clock coordinates cell physiological processes including inflammation. Recent studies suggested a crosstalk between these two pathways. However, mechanism of how inflammation affects is not well understood. Here, we investigated role proinflammatory transcription factor NF-κB in regulating function. Using combination genetic and pharmacological approaches, show that perturbation canonical subunit RELA human U2OS cellular model altered core gene expression. While...
Background. Progressive diseases including cancer, metabolic, and cardiovascular disorders are marked by platelet activation chronic inflammation. Studies suggest that dietary flavonoids such as quercetin possess antioxidant, anti-inflammatory, antiplatelet properties, which could prevent various atherosclerosis thrombosis. However, the mechanism signaling pathway links quercetin's activity with its anti-inflammatory property is limited thus further exploration required. The aim of this...
Abstract GTPase of immunity-associated protein 5 (Gimap5) is linked with lymphocyte survival, autoimmunity, and colitis, but its mechanisms action are unclear. Here, we show that Gimap5 essential for the inactivation glycogen synthase kinase-3β (GSK3β) following T cell activation. In absence Gimap5, constitutive GSK3β activity constrains c-Myc induction NFATc1 nuclear import, thereby limiting productive CD4 + proliferation. Additionally, facilitates Ser389 phosphorylation translocation...
Infants born prematurely worldwide have up to a 50% chance of developing bronchopulmonary dysplasia (BPD), clinical morbidity characterized by dysregulated lung alveolarization and microvascular development. It is known that PDGFR alpha-positive (PDGFRA+) fibroblasts are critical for PDGFRA+ reduced in BPD. A better understanding fibroblast heterogeneity functional activation status during pathogenesis required develop mesenchymal population-targeted therapies In this study, we utilized...
Torilin, a sesquiterpene isolated from the fruits of Torilis japonica, has shown antimicrobial, anticancer, and anti-inflammatory properties. However, data on mechanism torilin action against inflammation is limited. This study aimed at determining property in LPS-induced using vitro model inflammation. We examined torilin’s effect expression levels inflammatory mediators cytokines LPS-stimulated RAW 264.7 macrophages. The involvement NF-kB AP-1, MAP kinases, adaptor proteins were assessed....
Despite a multitude of reports on anti-inflammatory properties ginseng extracts or individual ginsenosides, data antiarthritic effect saponin preparation with mixed ginsenosides is limited. On the other hand, combined therapy safe and inexpensive plant-derived natural products such as can be considered an alternative to treat arthritis. Our previous in vitro displayed strong action red fraction-A (RGSF-A). We, herein, report marked property RGSF-A rich ginsenoside Rb1, Rc, Rb2....
Regulator of G protein signaling (RGS) family members, such as RGS2, interact with Galpha subunits heterotrimeric proteins, accelerating the rate GTP hydrolysis and attenuating intracellular triggered by protein-coupled receptor-ligand interaction. They are also reported to regulate protein-effector interactions form multiprotein complexes. Ischemic stress-induced changes in RGS2 expression have been described astrocytes, these associated cascades, suggesting that upregulation may be an...
Phellinus baumii is a mushroom that has been used as folk medicine against various diseases and reported to have antidiabetic, anticancer, antioxidant, antiinflammatory antihypertensive activities. However, information on the effects of P. extract in platelet function limited. Therefore, aim this study was examine impact methanol (PBME) activation investigate mechanism behind its antiplatelet activity. PBME agonist‐induced aggregation, granule secretion, [Ca 2+ ] i mobilization, α IIb β 3...
The following data are derived from key stages of acinar lung development and define the developmental role interstitial fibroblasts expressing platelet-derived growth factor alpha (PDGFRα). This dataset is related to research article entitled "Temporal, spatial, phenotypical changes PDGFRα during late development" (Endale et al., 2017) [1]. At E16.5 (canalicular), E18.5 (saccular), P7 (early alveolar) P28 (late alveolar), PDGFRαGFP mice, in conjunction with immunohistochemical markers, were...
Abstract Study Objectives Genetics impacts sleep, yet, the molecular mechanisms underlying sleep regulation remain elusive. In this study, we built machine learning models to predict genes based on their similarity that are known regulate sleep. Methods We trained a prediction model thousands of published datasets, representing circadian, immune, deprivation, and many other processes, using manually curated list 109 genes. Results Our predictions fit with prior knowledge identified key...
Background. Brucella abortus can proliferate within professional and nonprofessional phagocytic host cells thereby successfully bypass the bacteriocidal effects of phagocytes. However, intracellular survival mechanism factors virulence are not fully understood. Methods. We have investigated role regulator G protein signaling 2 (RGS2), an calcium ([Ca2+]i) cell, in B. Results. infection markedly induced RGS2 messenger RNA expression early phase increased [Ca2+]i level up to 24 hours...
Absence of MHC class I expression is an important mechanism by which NK cells recognize a variety target cells, yet the pathways underlying “missing‐self” recognition, including involvement activating receptors, remain poorly understood. Using ethyl‐ N ‐nitrosourea mutagenesis in mice, we identified germline mutant, designated Ace , with marked defect cell mediated recognition and elimination targets. The causative mutation was linked to chromosome 11 as missense (Thr428Ile) SH2 domain...
Heterozygous de novo or inherited gain-of-function mutations in the MTOR gene cause Smith-Kingsmore syndrome (SKS). SKS is a rare autosomal dominant condition, and individuals with display macrocephaly/megalencephaly, developmental delay, intellectual disability, seizures. A few dozen are reported literature. Here, we report cohort of 28 that represent nine pathogenic variants. We conducted detailed natural history study found pathophysiological deficits among addition to common...
Erysipelothrix rhusiopathiae is pathogenic for humans, many domestic animals and wild birds, but infectious cases with clinical symptoms in cats have not been reported. E. was recovered from a 4-month Russian blue breed cat very poor body condition score of 1 (BCS: 1/5). The isolate typed as serotype 2b. Mice experimentally infected the through subcutaneous or intraperitoneal routes survived, organism spleen synovial pericardial fluids. Cats inoculated either orally subcutaneously survived...
Suaeda asparagoides (Miq.) has long been used as a Korean folk herbal medicine for the treatment of functional gastrointestinal disorders. However, reports on its pharmacological activity motility are scarce. The present study investigated effects water fraction extract (SAWF) antral in vitro. Muscle strips from rat gastric antrum were set up an organ bath circular orientation. SAWF (100 µg/mL) inhibited spontaneous contraction muscle strips. These inhibitory not significantly affected by...