The combined use of multiple omics allows to study complex interrelated biological processes in their entirety. We applied a combination metabolomics, lipidomics and proteomics human bones investigate potential estimate time elapsed since death (i.e., the postmortem interval [PMI]). This ‘ForensOMICS’ approach has improve accuracy precision PMI estimation skeletonized remains, thereby helping forensic investigators establish timeline events surrounding death. Anterior midshaft tibial bone...

Bone proteomic studies using animal proxies and skeletonized human remains have delivered encouraging results in the search for potential biomarkers precise accurate post-mortem interval (PMI) age-at-death (AAD) estimation medico-legal investigations. The development of forensic proteomics PMI AAD is critical need research on throughout decomposition, as currently effects both inter-individual biological differences taphonomic alteration survival bone protein profiles are unclear. This study...

Abstract: Bone-mineral density (BMD) is a measure of the inorganic mineral content in bone, and one more informative assessments bone quality both clinical studies forensic investigations. Several factors, such as age, sex, disease, genetics, lifestyle, affect BMD measurements, normative standards must be applied for specific groups individuals. One most common disorders associated with low osteoporosis increased fracture risk, due to decrease strength an increase fragility. Medical...

Bone is a hard biological tissue and precious reservoir of information in forensic investigations as it retains key biomolecules commonly used for identification purposes. proteins have recently attracted significant interest their potential estimating post-mortem interval (PMI) age at death (AAD). However, the preservation such highly dependent on intrinsic extrinsic factors that can hinder application molecular techniques to sciences. The present study aims investigating effects two types...

The evaluation of bone diagenetic phenomena in archaeological timescales has a long history; however, little is known about the origins microbes driving diagenesis, nor extent diagenesis short timeframes-such as forensic contexts. Previously, analysis non-collagenous proteins (NCPs) through bottom-up proteomics revealed presence potential biomarkers useful estimating post-mortem interval (PMI). However, there still great need for enhancing understanding processes taking place timeframes, and...

The accurate estimation of postmortem interval (PMI), the time between death and discovery body, is crucial in forensic science investigations as it impacts legal outcomes. PMI extremely cold environments becomes susceptible to errors misinterpretations, especially with prolonged PMIs. This study addresses lack data on decomposition extreme by providing first overview such settings. Moreover, proposes microbiome prediction model for environments, applicable even when visual halted....

Considering the growing importance of microbiome analyses in forensics for identifying individuals, this study explores transfer skin onto clothing, its persistence on fabrics over time, and transferability from environment between different garments. Furthermore, project compares three specific QIAGEN extraction kits to test their efficiency fabric samples. Additionally, aims check if these extracts contain human DNA, providing a chance obtain more information same evidence personal...

Abstract Forensic anthropology includes, amongst other applications, the positive identification of unknown human skeletal remains. The first step in this process is an assessment biological profile, that is: sex, age, stature and ancestry. In forensic contexts, age estimation one main challenges identification. Recently established admissibility criteria are driving researchers towards standardisation methodological procedures. Despite these changes, experience still plays a central role...

Age estimation remains one of the most challenging tasks in forensic practice when establishing a biological profile unknown skeletonised remains. Morphological methods based on developmental markers bones can provide accurate age estimates at young age, but become highly unreliable for ages over 35 all disappear. This study explores changes biomechanical properties bone tissue and matrix, which continue to change with even after skeletal maturity, their potential value estimation. As proof...

Numerous intrinsic and extrinsic factors influence bone remodelling rates have shown to affect the accuracy of histological aging methods. The present study investigates rib cortex from two Mediterranean skeletal collections exploring development population-specific standards for histomorphometric age-at-death estimation. Eighty-eight standard ribs samples, Cretans Greek-Cypriots, were processed histologically. Thirteen raw composite parameters assessed observer error tested. correlation...

Measurement of the properties bone as a material can happen in various length scales its hierarchical and composite structure. The aim this study was to test tissue level clinically-relevant human samples which were collected from donors belonging three groups: ageing who suffered no fractures (Control); untreated fracture patients (Fx-Untreated) patient experienced hip despite being treated with bisphosphonates (Fx-BisTreated). Tissue assessed by (a) nanoindentation (b) synchrotron tensile...

Assessing the timing of skeletal trauma significantly impacts reconstruction events surrounding death and deposition in forensic cases. However, there are no absolute time frames which characteristics wet bone (peri-mortem) fractures transition to dry (post-mortem) fractures. The aim this study was attempt identify a point within post-mortem interval change from properties. A total 32 deer ribs were placed laboratory burial environment set three fractured with blunt force every week during...

The application of proteomic analysis to forensic skeletal remains has gained significant interest in improving biological and chronological estimations medico-legal investigations. To enhance the applicability these analyses casework, it is crucial maximize throughput proteome recovery while minimizing interoperator variability laboratory-induced post-translational protein modifications (PTMs). This work compared different workflows for extracting, purifying, analyzing bone proteins using...

Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) untargeted metabolomics has become the gold standard for profiling of low-molecular-weight compounds. Recently, this discipline raised great interest in forensic sciences, especially field toxicology and

The study of post-mortem changes is a crucial component forensic investigation. Human taphonomic facilities (HFTFs) are the only institutions allowing design and execution controlled human decomposition experiments. When bodies skeletonized, bones normally stored in skeletal collections used for anthropological studies. However, HFTFs apply chemical and/or thermal treatments to remains prior bone long-term storage. These believed alter heavily original biochemical molecular signature...

This project is an innovative mixture of medical imaging and forensics with holography, animation, art virtual reality. alliance science explores, presents communicates the history violence to diverse audiences. Anatomical detail provides substrate for forensic analysis interpretation while digital technologies creative nourish fictitious scenarios on unexplained historical crimes. The aesthetics world create immersive environment emotional, ethical aesthetic impact becomes a site...