A5060845383- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Genetics and Physical Performance
- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
- Dietary Effects on Health
- High Altitude and Hypoxia
- Antimicrobial Peptides and Activities
- Nerve injury and regeneration
- Connexins and lens biology
- Nuclear Receptors and Signaling
- Cancer-related gene regulation
- Cardiovascular Effects of Exercise
- RNA modifications and cancer
Federal Medical-Biological Agency
2018-2025
Moscow Institute of Physics and Technology
2016-2022
Moscow Power Engineering Institute
2022
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2018-2019
Institute of Bioorganic Chemistry
2018
Abstract Skeletal muscle tissue demonstrates global hypermethylation with age. However, methylome changes across the time-course of differentiation in aged human derived cells, and larger coverage arrays have not been undertaken. Using 850K DNA methylation we compared methylomes young (27 ± 4.4 years) (83 4 skeletal that young/aged heterogenous muscle-derived primary cells (HDMCs) over several time points (0, 72 h, 7, 10 days). Aged was hypermethylated tissue, enriched for;...
Abstract Whole-exome sequencing is an attractive alternative to microarray analysis because of the low cost and potential ability detect copy number variations (CNV) various sizes (from 1–2 exons several Mb). Previous comparison most popular CNV calling tools showed a high portion false-positive calls. Moreover, due lack gold standard set, results are limited incomparable. Here, we aimed perform comprehensive capable germline available at moment using single reference sample set. Compiling...
Muscle fiber composition is associated with physical performance, endurance athletes having a high proportion of slow-twitch muscle fibers compared to power athletes. Approximately 45% heritable, however, single nucleotide polymorphisms (SNP) underlying inter-individual differences in types remain largely unknown. Based on three whole genome SNP datasets, we have shown that the rs236448 A allele located near cyclin-dependent kinase inhibitor 1A (CDKN1A) gene was an increased Russian (n =...
Abstract Purpose Recent research suggests a link between brain-derived neurotrophic factor (BDNF) and the mTOR signaling pathway, key regulator of protein synthesis muscle growth. However, it remains unclear whether BDNF influences growth power performance. Our study aimed to investigate relationship expression MTOR genes in human skeletal examine association genetically predicted higher gene fiber size, lean mass, performance, athlete status. Methods The involved 456,382 subjects, including...
Nuclear noncoding RNAs (ncRNAs) are key regulators of gene expression and chromatin organization. The progress in studying nuclear ncRNAs depends on the ability to identify genome-wide spectrum contacts with chromatin. To address this question, a panel RNA-DNA proximity ligation techniques has been developed. However, neither these examines proteins involved RNA-chromatin interactions. Here, we introduce RedChIP, technique combining immunoprecipitation for identifying interactions mediated...
G-quadruplex (G4) sites in the human genome frequently colocalize with CCCTC-binding factor (CTCF)-bound CpG islands (CGIs). We aimed to clarify role of G4s CTCF positioning. Molecular modeling data suggested direct interactions, so we performed vitro binding assays quadruplex-forming sequences from CGIs genome. bound Kd values similar that control duplex, while respective i-motifs exhibited no affinity for CTCF. Using ChIP-qPCR assays, showed G4-stabilizing ligands enhance occupancy at a...
Abstract Insertions and deletions (indels) are the second most common type of variation in human genome. However, limited data on their associations with exercise‐related phenotypes have been documented. The aim present study was to examine association between 18,370 indel variants power athlete status, followed by additional studies 357,246 individuals. In discovery phase, D allele MDM4 gene rs35493922 I/D polymorphism over‐represented athletes compared control subjects ( P = 7.8 × 10 −9 )...
A bstract Background Prostate adenocarcinoma (PRAD) is the second leading cause of cancer-related deaths in men. High variability DNA methylation and a high rate large genomic rearrangements are often observed PRAD. Results To investigate reasons for such variance, we integrated methylation, RNA-seq, copy number alterations datasets from The Cancer Genome Atlas (TCGA), focusing on PRAD, employed weighted gene co-expression network analysis (WGCNA). Our results show that only single cluster...
In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread species that infects almost all crops. Different P. strains use wide range of biochemical mechanisms, including phytotoxins and effectors type III IV secretion systems, which determine specific nature pathogen virulence.Strains 1845 (isolated from dicots) 2507 monocots) were selected for sequencing because they specialize on different groups plants. We...
Although modern methods of whole genome DNA methylation analysis have a wide range applications, they are not suitable for clinical diagnostics due to their high cost and complexity the large amount sample required analysis. Therefore, it is crucial be able identify relatively small number sites that provide precision sensitivity diagnosis pathological states. We propose an algorithm constructing limited subsamples from high-dimensional data form diagnostic panels. developed tool utilizes...
There is a clear need in molecular markers for prostate cancer (PC) risk stratification. Alteration of DNA methylation one processes that occur during ÐÑ progression. Methylation-sensitive PCR with high resolution melting curve analysis (MS-HRM) can be used gene routine laboratory practice. This method requires very small amounts analysis. Numerous results have been accumulated on PC samples analyzed by the Infinium HumanMethylation450 BeadChip (HM450). However, consistency MS-HRM chip...
The genetic reprogramming technology allows generation of induced pluripotent stem cells (iPSCs) from somatic (Takahashi and Yamanaka, 2006) [1]. iPSCs have the ability to self-renew, differentiate into any type cells, are considered as a promising tool for drug development, disease modeling, regenerative medicine. factors (oct4, sox2, klf4, c-myc) can be delivered cell nucleus either by vectors integrating genome (lentiviruses, retroviruses) or non-integrative methods (e.g., plasmids,...
BACKGROUND: Decreased skeletal muscle mass and properties lead to the development of various pathologies increased risk for injuries. Studies molecular mechanisms adaptation resistance training increase strength appear imperative medicine sports. AIM: To assess changes in proteomic profile (quantitative panoramic spectrometric analysis) muscles correlation these with expression corresponding mRNAs (RNA-sequencing) before after 12 weeks transcriptome 8 24 h an acute exercise one leg. METHODS:...
BACKGROUND: Based on the most recent findings, E3 ubiquitin ligase TRIM29 participates in numerous cellular processes and holds significance both formation of normal basal epithelium prostate cancer. Exploring these molecular mechanisms could be pivotal developing novel cancer therapies. AIM: Identification protein partners its truncated forms, followed by functional analysis proteins. key involving TRIM29. METHODS: Cell cultures from with overexpression chimeric TRIM29-FLAG or forms lacking...
Background: Data on the genetic factors contributing to inter-individual variability in muscle fiber size are limited. Recent research has demonstrated that mice lacking Arkadia (RNF111) N-terminal-like PKA signaling regulator 2N (Ark2n; also known as C18orf25) gene exhibit reduced size, contraction force, and exercise capacity, along with defects calcium handling within fast-twitch fibers. However, role of ARK2N human physiology, particularly athletic populations, remains poorly understood....
Abstract Small mammals rely on type IIb myofibers, expressing the fastest myosin (encoded by MYH4 ), for rapid muscle contraction. In contrast, larger mammals, including humans, show reduced or absent expression and favoring slower-contracting myofibers. The evolutionary mechanisms underlying this shift remain unclear. Here, we identify large MAF transcription factors (MAFA, MAFB, MAF) as key regulators of in human bovine. Overexpression MAFs induces enhances glycolytic capacity myotubes,...
Aerobic physical exercise has significant benefits for cardiovascular health; however, some individuals experience no benefit or even adverse effects. One reason poor tolerance to aerobic may be a low percentage of slow-twitch (oxidative) muscle fibers. This study aims identify the metabolic signatures associated with and high response by comparing profiles participants categorized according their improvement 6-minute walking distance. In this study, pre- postexercise intervention...